Abstract
Feniletanoit glikozitler, çeşitli biyolojik aktivitelere sahip olduğu bildirilen doğal bileşiklerdir. Bu çalışmada Stachys macrantha’dan izole edilen lavandulifoliozit, verbaskozit, leukoseptozit A and martinozit’in insan lenfositlerinde mutajenik ve/veya antimutajenik aktiviteleri tek hücre jel elektroforez veya “comet” yöntemi ile incelendi. Mitomisin C varlığında veya yokluğunda lenfositler feniletanoit glikozitlerin farklı konsantrasyonları ile inkübe edildi ve DNA zincir kırıkları comet yöntemi ile ölçüldü. Feniletanoit glikozitlerin 10, 50, 100 ve 200 μg/ml konsantrasyonlarında artan DNA hasarı gözlenmemiştir, oysa mitomisin C ile indüklenen hasarlı hücre sıklığı feniletanoit glikozitlerin düşük konsantrasyonları ile muamelesinde anlamlı olarak azalmıştır. Ancak 200 μg/ml konsantrasyonların yukarısında koruyucu etki gözlenmemiştir. İnsan lenfositlerinde feniletanoit glikozitlerin mitomisin C ile indüklenen DNA hasarına karşı konsantrasyona bağımlı olarak koruyucu etki gösterdiği bulunmuştur.
The Protective Effects of Some Phenylethanoid Glycosides on the Mitomycin C Induced DNA Strand Breakage
Abstract
Phenylethanoid glycosides represent a group of natural products, which have been reported to have various biological activities. In this study the mutagenic and/or antimutagenic activities of lavandulifolioside, verbascoside, leucosceptoside A and martynoside isolated from Stachys macrantha were investigated in human lymphocytes by single cell gel electrophoresis or ‘comet’ assay. The lymphocytes incubated with different concentrations of phenylethanoid glycosides with or without mitomycin C and DNA strand breakage was measured by comet assay. At the phenylethanoid glycoside concentrations of 10, 50, 100, and 200 μg/ml no additional DNA damage was observed whereas the frequency of damaged cells induced by mitomycin C was significantly decreased by phenylethanoid glycoside treatment. However at the highest concentrations above 200 μg/ml no protective effect was observed. It seemed that in human lymphocytes phenylethanoid glycosides showed protective effects on DNA damage induced by mitomycin C in a concentration dependent manner.
Details
| Primary Language | English |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | January 1, 2004 |
| Published in Issue | Year 2004 Issue: 1 |