Year 2021, Volume 41 , Issue 1, Pages 45 - 63 2021-01-01

Bir ilaç etkin maddesinin, kozmetik ürünün, çevresel bir kimyasalın (pestisitler, endokrin bozucular, ağır metaller, nanopartiküller gibi), ağır metallerin ve fiziksel veya biyolojik ajanların in vitro olarak sitotoksik etkilerinin güncel yöntemlerle belirlenmesi son yıllarda oldukça önem kazanmıştır. Sitotoksisitenin veya hücre canlılığının belirlenmesi için birçok yöntem bulunmaktadır. Bu yöntemler (i) boyama yöntemleri; (ii) kolorimetrik yöntemler; (iii) florometrik yöntemler; (iv) luminometrik yöntemler; (v) apoptozun belirlenmesi için kullanılan farklı teknikler ve (vi) otofajinin belirlenmesi için kullanılan farklı teknikler olarak sınıflandırılabilir. Bu yöntemlerin hangisinin en uygun yöntem olduğu araştırıcı tarafından tüm bilimsel veriler kullanılarak değerlendirilmelidir. Bu değerlendirme yapılırken test maddesinin fiziksel ve kimyasal özellikleri, maddenin hangi mekanizma ile hücre ölümüne yol açtığı, yönteminin özgünlüğü ve hassasiyeti dikkate alınmalıdır. Bu şekilde elde edilen sonucun güvenirliliği ve doğruluğu konusunda emin olunmalıdır. Bu derlemede, günümüzde in vitro olarak sitotoksisitenin belirlenmesinde sıklıkla kullanılan güncel yöntemler ayrıntılı olarak anlatılmış ve bu yöntemlerin üstünlük ve dezavantajlarından derlenmiştir.
Sitotoksisite, hücre canlılığı
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Primary Language tr
Subjects Pharmacology and Pharmacy
Journal Section Review Articles
Authors

Author: Pınar ERKEKOĞLU (Primary Author)
Institution: HACETTEPE UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF TOXICOLOGY
Country: Turkey


Author: Terken BAYDAR
Institution: HACETTEPE UNIVERSITY, FACULTY OF PHARMACY, DEPARTMENT OF PROFESSIONAL PHARMACEUTICAL SCIENCES, DEPARTMENT OF TOXICOLOGY
Country: Turkey


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Project Number ---
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Dates

Publication Date : January 1, 2021

Bibtex @review { hujpharm729993, journal = {Hacettepe University Journal of the Faculty of Pharmacy}, issn = {}, eissn = {2458-8806}, address = {hujpharm@gmail.com}, publisher = {Hacettepe University}, year = {2021}, volume = {41}, pages = {45 - 63}, doi = {}, title = {Güncel İn Vitro Sitotoksisite Testleri}, key = {cite}, author = {Erkekoğlu, Pınar and Baydar, Terken} }
APA Erkekoğlu, P , Baydar, T . (2021). Güncel İn Vitro Sitotoksisite Testleri . Hacettepe University Journal of the Faculty of Pharmacy , 41 (1) , 45-63 . Retrieved from https://dergipark.org.tr/en/pub/hujpharm/issue/60867/729993
MLA Erkekoğlu, P , Baydar, T . "Güncel İn Vitro Sitotoksisite Testleri" . Hacettepe University Journal of the Faculty of Pharmacy 41 (2021 ): 45-63 <https://dergipark.org.tr/en/pub/hujpharm/issue/60867/729993>
Chicago Erkekoğlu, P , Baydar, T . "Güncel İn Vitro Sitotoksisite Testleri". Hacettepe University Journal of the Faculty of Pharmacy 41 (2021 ): 45-63
RIS TY - JOUR T1 - Güncel İn Vitro Sitotoksisite Testleri AU - Pınar Erkekoğlu , Terken Baydar Y1 - 2021 PY - 2021 N1 - DO - T2 - Hacettepe University Journal of the Faculty of Pharmacy JF - Journal JO - JOR SP - 45 EP - 63 VL - 41 IS - 1 SN - -2458-8806 M3 - UR - Y2 - 2020 ER -
EndNote %0 Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi Güncel İn Vitro Sitotoksisite Testleri %A Pınar Erkekoğlu , Terken Baydar %T Güncel İn Vitro Sitotoksisite Testleri %D 2021 %J Hacettepe University Journal of the Faculty of Pharmacy %P -2458-8806 %V 41 %N 1 %R %U
ISNAD Erkekoğlu, Pınar , Baydar, Terken . "Güncel İn Vitro Sitotoksisite Testleri". Hacettepe University Journal of the Faculty of Pharmacy 41 / 1 (January 2021): 45-63 .
AMA Erkekoğlu P , Baydar T . Güncel İn Vitro Sitotoksisite Testleri. HUJPHARM. 2021; 41(1): 45-63.
Vancouver Erkekoğlu P , Baydar T . Güncel İn Vitro Sitotoksisite Testleri. Hacettepe University Journal of the Faculty of Pharmacy. 2021; 41(1): 45-63.
IEEE P. Erkekoğlu and T. Baydar , "Güncel İn Vitro Sitotoksisite Testleri", Hacettepe University Journal of the Faculty of Pharmacy, vol. 41, no. 1, pp. 45-63, Jan. 2021