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Bitkisel Lipaz Kaynakları ve Kullanım Alanları

Year 2021, Volume: 41 Issue: 1, 35 - 44, 01.01.2021

Abstract

Enzimler; substrata özgü olan, hücre içinde ve dışında birçok biyolojik reaksiyonda görev alan protein yapısındaki biyokatalizörlerdir. Lipazlar (triaçilgliserol açil hidrolazlar), gliserol ve uzun zincirli yağ asitlerinden oluşan esterlerin hidrolizini ve sentezini katalizleyen enzimlerdir. Diğer sentetik katalizörlere kıyasla toksik olmayan ve çevre dostu olan lipazların kullanımları gün geçtikçe artmaktadır. Günümüzde geldiğimiz Endüstri 4.0 sürecine uyum sağlayan gıda, kimya, yakıt, kağıt ve selüloz, ilaç, kozmetik, parfüm ve temizlik endüstrilerinde; gelişen biyoteknolojik uygulamalardan da güç alarak geleneksel yöntemlerin yerine enzimlerin kullanıldığı yeni nesil uygulamalara geçilmiştir. Bu noktada; kullanılan/geliştirilen basit, duyarlı, hızlı ve spesifik enzim saflaştırma metodlarıyla elde edilen aşırı koşullarda sürdürülebilirlik yeteneğine sahip lipazların önemi gün geçtikçe artmaktadır. Bu derlemede, lipaz enziminin uygulama alanlarından bahsedilmiş, üretiminde yüksek enzim maliyetlerini hafifletebilecek doğal kaynakların çeşitliliğine dikkat çekilmiştir.

References

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Herbal Lipase Sources and Uses

Year 2021, Volume: 41 Issue: 1, 35 - 44, 01.01.2021

Abstract

Enzymes; They are protein catalyst biocatalysts that are specific to the substrate and are involved in many biological reactions inside and outside the cell. Lipases (triacylglycerol acyl hydrolases) are enzymes that catalyze the hydrolysis and synthesis of esters consisting of glycerol and long chain fatty acids. The use of non-toxic and environmentally friendly lipases is increasing day by day compared to other synthetic catalysts. In the food, chemical, fuel, paper and cellulose, pharmaceutical, cosmetic, perfume and cleaning industries, which adapt to the Industry 4.0 process we have reached today; By taking power from the developing biotechnological applications, new generation applications were used in which enzymes were used instead of traditional methods. At this point; The importance of lipases that are capable of sustainability in extreme conditions obtained by simple, sensitive, fast and specific enzyme purification methods used / developed is increasing day by day. In this review, the application areas of the lipase enzyme are mentioned and attention is drawn to the diversity of natural resources that can alleviate high enzyme costs in its production.

References

  • 1. Bernards MA: Plant natural products: a primer. Canadian Journal of Zoology 2010, 88(7): 601-614.
  • 2. Barbosa O, Ortiz C, Berenguer-Murcia Á, Torres R, Rodrigues RC, Fernandez-Lafuente R: Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization. Royal Society of Chemistry 2014, 4: 1583-1600.
  • 3. Jawed A, Singh G, Kohli S, Sumera A, Haque S, Prasad R, Paul D: Therapeutic role of lipases and lipase inhibitors derived from natural resources for remedies against metabolic disorders and lifestyle diseases. South African Journal of Botany 2019, 120: 25-32.
  • 4. Patil KJ, Chopda MZ, Mahajan RT: Lipase biodiversity. Indian Journal of Science and Technology 2011, 4(8): 971-982.
  • 5. Hasan F, Shah AA, Hameed A: Industrial applications of microbial lipases. Enzyme and Microbial Technology 2006, 39(2): 235-251.
  • 6. Zhu X, Ye A, Verrier T, Singh H: Free fatty acid profiles of emulsified lipids during in vitro digestion with pancreatic lipase. Food Chemistry 2013, 139(1-4): 398-404.
  • 7. Mercan Ülkü D: Antep fıstığı bitkisinden (Pistacia vera L.) lipaz enzimi saflaştırılması ve kinetik özelliklerinin incelenmesi (Yüksek Lisans Tezi, Kütahya Dumlupınar Üniversitesi/Fen Bilimleri Enstitüsü) 2018.
  • 8. Mounguengui RWM, Brunschwig C, Baréa B, Villeneuve P, Blin J: Are plant lipases a promising alternative to catalyze transesterification for biodiesel production?. Progress in Energy and Combustion Science 2013, 39(5): 441-456.
  • 9. Nelson DL, Cox MM: Lehninger biyokimyanın ilkeleri (Çevirici: Nedret K.), Palme Yayıncılık; Ankara, Türkiye, 2005.
  • 10. Gonçalves-Filho D, Silva AG, Guidini CZ: Lipases: sources, immobilization methods, and industrial applications. Applied Microbiology and Biotechnology 2019, 103(18): 7399-7423.
  • 11. Villeneuve P, Muderhwa JM, Graille J, Haas MJ: Customizing lipases for biocatalysis: a survey of chemical, physical and molecular biological approaches. Journal of Molecular Catalysis B:Enzymatic 2000, 9(4-6): 113-148.
  • 12. Messaoudi A, Belguith H, Gram I, Hamida JB: Classification of EC 3.1.1.3 bacterial true lipases using phylogenetic analysis. African Journal of Biotechnology 2010, 9(48): 8243-8247.
  • 13. Koç MY: Geobacillus türlerinde termostabil lipaz üretimi (Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Anabilim Dalı) 2013.
  • 14. Avramiuc M: Comparatıve Study On The Lıpase Actıvıty From Plant Sources, Under Varıous Condıtıons Of Ph, Temperature And Substrate. Food And Environment Safety Journal 2016, 15(1): 21-28.
  • 15. Javed S, Azeem F, Hussain S, Rasul I, Siddique MH, Riaz M, Afzal M, Kouser A, Nadeem H: Bacterial lipases: A review on purification and characterization. Progress in Biophysics and Molecular Biology 2018, 132: 23-34.
  • 16. Mehta A, Bodh U, Gupta R: Fungal lipases: a review. Journal of Biotech Research 2017, 8: 58-77. 17. Öztürk B: Lipaz enzimi: Yapısal özellikleri ve uygulama alanları. Gıda mühendisliği Dergisi 2002, 12: 20-23.
  • 18. Ugo AK, Amara AV, Kenechuwku U, Igwe CN: Microbial lipases: a prospect for biotechnological industrial catalysis for green products: a review. Fermentation Technology 2017, 6(144): 2-12.
  • 19. Gunasekaran V, Das D: Lipase fermentation: progress and prospects. Indian Journal of Biotechnology 2005, 4: 437-445.
  • 20. Özcan C. Lipaz (LPS) nedir? Lipaz düşüklüğü ve yüksekliği ne anlama gelir?; 2020 March 5. Available from: https://www.medicalpark.com.tr/lipaz/hg-2194 [Website]
  • 21. Tursi JM, Phair PG, Barnes GL: Plant sources of acid stable lipases: potential therapy for cystic fibrosis. Journal of Paediatrics and Child Health 1994, 30(6): 539-543.
  • 22. Roxas M: The role of enzyme supplementation in digestive disorders. Alternative Medicine Review 2008, 13(4): 307-314.
  • 23. Thakur S: Lipases, its sources, properties and applications: a review. International Journal of Scientific & Engineering Research 2012, 3(7): 1-29.
  • 24. Babu IS, Rao GH: Optimization of process parameters for the production of lipase in submerged fermentation by Yarrowia lipolytica NCIM 3589. Research Journal of Microbiology 2007, 2(1): 88-93.
  • 25. Li CY, Cheng CY, Chen TL: Fed-batch production of lipase by Acinetobacter radioresistens using Tween 80 as the carbon source. Biochemical Engineering Journal 2004, 19(1): 25-31.
  • 26. Rai S, Vasava H, Upadhaya D: Bacterial Lipase: A Review. Studies in Indian Place Names 2020, 40(71): 200-208.
  • 27. Dutta S, Ray L: Production and characterization of an alkaline thermostable crude lipase from an isolated strain of Bacillus cereus C7. Applied Biochemistry and Biotechnology 2009, 159(1): 142-154.
  • 28. Mnisi SM, Louw ME, Theron J: Cloning and characterization of a carboxylesterase from Bacillus coagulans 81-11. Current Microbiology 2005, 50(4): 196-201.
  • 29. Ramchuran SO, Vargas VA, Hatti-Kaul R, Karlsson EN: Production of a lipolytic enzyme originating from Bacillus halodurans LBB2 in the methylotrophic yeast Pichia pastoris. Applied Microbiology and Biotechnology 2006, 71(4): 463-472.
  • 30. Sangeetha R, Geetha A, Arulpandi I: Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization. Brazilian Journal of Microbiology 2010, 41(1): 179-185.
  • 31. Sangeetha R, Geetha A, Arulpandi I: Concomitant production, partial purification and characterization of a serine protease and a proteolysis-resistant metallolipase from Bacillus pumilus SG2. Zeithschrift für Naturforschung C 2010, 65(1-2): 61-65.
  • 32. Ahmed EH, Raghavendra T, Madamwar D: A thermostable alkaline lipase from a local isolate Bacillus subtilis EH 37: characterization, partial purification, and application in organic synthesis. Applied Biochemistry and Biotechnology 2010, 160(7): 2102-2113.
  • 33. Quyen DT, Schmidt-Dannert C, Schmid RD: High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expression and Purification 2003, 28(1): 102-110.
  • 34. Wang X, Yu X, Xu Y: Homologous expression, purification and characterization of a novel high-alkaline and thermal stable lipase from Burkholderia cepacia ATCC 25416. Enzyme and Microbial Technology 2009, 45(2): 94-102.
  • 35. Arpigny JL, Jaeger KE: Bacterial lipolytic enzymes: classification and properties. Biochemical Journal 1999, 343(1): 177-183.
  • 36. Long ZD, Xu JH, Zhao LL, Pan J, Yang S, Hua L: Overexpression of Serratia marcescens lipase in Escherichia coli for efficient bioresolution of racemic ketoprofen. Journal of Molecular Catalysis B: Enzymatic 2007, 47(3-4): 105-110.
  • 37. Madan B, Mishra P: Co-expression of the lipase and foldase of Pseudomonas aeruginosa to a functional lipase in Escherichia coli. Applied Microbiology and Biotechnology 2010, 85(3): 597-604.
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There are 84 citations in total.

Details

Primary Language Turkish
Subjects Pharmacology and Pharmaceutical Sciences
Journal Section Review Articles
Authors

Seren Gündoğdu

Ayşe Uz 0000-0003-3373-4759

Publication Date January 1, 2021
Acceptance Date February 23, 2021
Published in Issue Year 2021 Volume: 41 Issue: 1

Cite

Vancouver Gündoğdu S, Uz A. Bitkisel Lipaz Kaynakları ve Kullanım Alanları. HUJPHARM. 2021;41(1):35-44.