Year 2021, Volume 41 , Issue 1, Pages 9 - 22 2021-01-01

Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract
Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract

Ahmed Algali SEDAHMED [1] , Mosab Yahya AL-NOUR [2] , Mirghani Hashim MIRGHANI [3] , Hussam Eldeen ABUALGASIM1 [4] , Fadl Albaseer Alamin ALTIB [5] , Ahmed ADİL ALI [6] , Esraa ELHADI [7] , Ahmed Haasan ARBAB [8]


Relying on the previous literature, Albizia amara possesses phytochemical constituents having significant pharmacological activities. Our study preliminary screened the phytochemical constituents and investigated the potential anticonvulsant activity using the Pilocarpine-induced seizures on albino rats. The qualitative phytochemical screening of the extract indicated the presence of flavonoids, alkaloids, saponins, tannins, and sterols. The anticonvulsant activity assessment revealed that the extract (400 mg/kg I.P.) increased the latency to the first seizure and significantly decreased the duration of the seizure. Moreover, the extract reduced the mortality rate during the first observed 24 hours. Furthermore, to predict the probable mechanism of action, in silico study was conducted. The results indicated that predicted compounds that have the highest contribution in the activity are Budmunchiamine A, 1, 2-benzenedicarboxylic acid, mono (2-Ethylhexyl) ester, Hexadecanoic acid methyl ester, and Pithecolobine. The predicted targets that involved in the antiepileptic activity are neural acetylcholine receptor subunits alpha-4 and alpha-7, 5-hydroxytryptamine receptor 3A, Cannabinoid receptor 1, kinase C gamma & epsilon subunits, and Glutamate carboxypeptidase-2 enzyme. Unfortunately, Budmunchiamine A was predicted to be Cardiotoxic and Hepatotoxic. Pithecolobine was predicted to be Cardiotoxic. In conclusion, the findings demonstrate the potentiality of A. amara’s ethanolic leaves extract in the attenuation of Pilocarpine induced convulsion.
Relying on the previous literature, Albizia amara possesses phytochemical constituents having significant pharmacological activities. Our study preliminary screened the phytochemical constituents and investigated the potential anticonvulsant activity using the Pilocarpine-induced seizures on albino rats. The qualitative phytochemical screening of the extract indicated the presence of flavonoids, alkaloids, saponins, tannins, and sterols. The anticonvulsant activity assessment revealed that the extract (400 mg/kg I.P.) increased the latency to the first seizure and significantly decreased the duration of the seizure. Moreover, the extract reduced the mortality rate during the first observed 24 hours. Furthermore, to predict the probable mechanism of action, in silico study was conducted. The results indicated that predicted compounds that have the highest contribution in the activity are Budmunchiamine A, 1, 2-benzenedicarboxylic acid, mono (2-Ethylhexyl) ester, Hexadecanoic acid methyl ester, and Pithecolobine. The predicted targets that involved in the antiepileptic activity are neural acetylcholine receptor subunits alpha-4 and alpha-7, 5-hydroxytryptamine receptor 3A, Cannabinoid receptor 1, kinase C gamma & epsilon subunits, and Glutamate carboxypeptidase-2 enzyme. Unfortunately, Budmunchiamine A was predicted to be Cardiotoxic and Hepatotoxic. Pithecolobine was predicted to be Cardiotoxic. In conclusion, the findings demonstrate the potentiality of A. amara’s ethanolic leaves extract in the attenuation of Pilocarpine induced convulsion.
  • 1 Wahab, A. Difficulties in Treatment and Management of Epilepsy and Challenges in New Drug Development. Pharmaceuticals (Basel). 2010;3: 2090-2110.
  • 2 McCagh, J. in Epilepsy Topics (ed Mark D. Holmes) (July 16th 2014).
  • 3 JA, D. Mechanisms of action of antiepileptic drugs. Seizure. 1995;4, 267-271.
Primary Language en
Subjects Pharmacology and Pharmacy
Journal Section Research Articles
Authors

Orcid: 0000-0002-2296-1350
Author: Ahmed Algali SEDAHMED (Primary Author)
Institution: Department of pharmacology , institute of health sciences , hacettepe university
Country: Turkey


Orcid: 0000-0002-5044-3948
Author: Mosab Yahya AL-NOUR
Institution: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Omdurman Islamic University
Country: Sudan


Orcid: 0000-0002-9600-9514
Author: Mirghani Hashim MIRGHANI
Institution: Faculty of Pharmacy, Omdurman Islamic University, Khartoum, Sudan
Country: Sudan


Orcid: 0000-0001-9376-2466
Author: Hussam Eldeen ABUALGASIM1
Institution: Faculty of Pharmacy, Omdurman Islamic University
Country: Sudan


Orcid: 0000-0003-0865-0115
Author: Fadl Albaseer Alamin ALTIB
Institution: Department of Pharmacology, Faculty of Pharmacy, Omdurman Islamic University
Country: Sudan


Orcid: 0000-0002-7617-5590
Author: Ahmed ADİL ALI
Institution: Faculty of Pharmacy, Omdurman Islamic University, Khartoum, Sudan
Country: Sudan


Orcid: 0000-0003-4137-0086
Author: Esraa ELHADI
Institution: Department of Pharmacology, school of pharmaceutical sciences, China Pharmaceutical University
Country: China


Orcid: 0000-0002-5975-112X
Author: Ahmed Haasan ARBAB
Institution: Department of Pharmacognosy, Faculty of Pharmacy, Khartoum University
Country: Sudan


Dates

Publication Date : January 1, 2021

Bibtex @research article { hujpharm861481, journal = {Hacettepe University Journal of the Faculty of Pharmacy}, issn = {}, eissn = {2458-8806}, address = {hujpharm@gmail.com}, publisher = {Hacettepe University}, year = {2021}, volume = {41}, pages = {9 - 22}, doi = {}, title = {Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract}, key = {cite}, author = {Sedahmed, Ahmed Algali and Al-nour, Mosab Yahya and Mırghanı, Mirghani Hashim and Abualgasım1, Hussam Eldeen and Altıb, Fadl Albaseer Alamin and Adil Alı, Ahmed and Elhadı, Esraa and Arbab, Ahmed Haasan} }
APA Sedahmed, A , Al-nour, M , Mırghanı, M , Abualgasım1, H , Altıb, F , Adil Alı, A , Elhadı, E , Arbab, A . (2021). Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract . Hacettepe University Journal of the Faculty of Pharmacy , 41 (1) , 9-22 . Retrieved from https://dergipark.org.tr/en/pub/hujpharm/issue/60867/861481
MLA Sedahmed, A , Al-nour, M , Mırghanı, M , Abualgasım1, H , Altıb, F , Adil Alı, A , Elhadı, E , Arbab, A . "Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract" . Hacettepe University Journal of the Faculty of Pharmacy 41 (2021 ): 9-22 <https://dergipark.org.tr/en/pub/hujpharm/issue/60867/861481>
Chicago Sedahmed, A , Al-nour, M , Mırghanı, M , Abualgasım1, H , Altıb, F , Adil Alı, A , Elhadı, E , Arbab, A . "Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract". Hacettepe University Journal of the Faculty of Pharmacy 41 (2021 ): 9-22
RIS TY - JOUR T1 - Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract AU - Ahmed Algali Sedahmed , Mosab Yahya Al-nour , Mirghani Hashim Mırghanı , Hussam Eldeen Abualgasım1 , Fadl Albaseer Alamin Altıb , Ahmed Adil Alı , Esraa Elhadı , Ahmed Haasan Arbab Y1 - 2021 PY - 2021 N1 - DO - T2 - Hacettepe University Journal of the Faculty of Pharmacy JF - Journal JO - JOR SP - 9 EP - 22 VL - 41 IS - 1 SN - -2458-8806 M3 - UR - Y2 - 2021 ER -
EndNote %0 Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract %A Ahmed Algali Sedahmed , Mosab Yahya Al-nour , Mirghani Hashim Mırghanı , Hussam Eldeen Abualgasım1 , Fadl Albaseer Alamin Altıb , Ahmed Adil Alı , Esraa Elhadı , Ahmed Haasan Arbab %T Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract %D 2021 %J Hacettepe University Journal of the Faculty of Pharmacy %P -2458-8806 %V 41 %N 1 %R %U
ISNAD Sedahmed, Ahmed Algali , Al-nour, Mosab Yahya , Mırghanı, Mirghani Hashim , Abualgasım1, Hussam Eldeen , Altıb, Fadl Albaseer Alamin , Adil Alı, Ahmed , Elhadı, Esraa , Arbab, Ahmed Haasan . "Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract". Hacettepe University Journal of the Faculty of Pharmacy 41 / 1 (January 2021): 9-22 .
AMA Sedahmed A , Al-nour M , Mırghanı M , Abualgasım1 H , Altıb F , Adil Alı A , Elhadı E , Arbab A . Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract. HUJPHARM. 2021; 41(1): 9-22.
Vancouver Sedahmed A , Al-nour M , Mırghanı M , Abualgasım1 H , Altıb F , Adil Alı A , Elhadı E , Arbab A . Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract. Hacettepe University Journal of the Faculty of Pharmacy. 2021; 41(1): 9-22.
IEEE A. Sedahmed , M. Al-nour , M. Mırghanı , H. Abualgasım1 , F. Altıb , A. Adil Alı , E. Elhadı and A. Arbab , "Phytochemical, in vivo, and in silico Anticonvulsant Activity Screening of Albizia Amara Leave’s Ethanolic Extract", Hacettepe University Journal of the Faculty of Pharmacy, vol. 41, no. 1, pp. 9-22, Jan. 2021

Authors of the Article
Mosab Yahya AL-NOUR [2]
Mirghani Hashim MIRGHANI [3]
Hussam Eldeen ABUALGASIM1 [4]
Fadl Albaseer Alamin ALTIB [5]
Ahmed ADİL ALI [6]
Esraa ELHADI [7]
Ahmed Haasan ARBAB [8]