Antiviral Potential of Cistus L. against Infectious Bronchitis Virus and SARS-CoV-2

Year 2025, Volume: 45 Issue: 1, 1 - 17, 01.03.2025

Feyruz Bozoglan , Ali Ergüç , Furkan Ozan Çöven , Gizem Gungor Erkanli , Engin Alp Onen , Ahmet C Goren , Ayşe Nalbantsoy

https://doi.org/10.52794/hujpharm.1525333

Abstract

Cistus L. (Cistus) is distributed in Türkiye by 5 species including Cistus creticus L. (C. creticus), Cistus monspeliensis L. (C. monspeliensis), Cistus laurifolius L. (C. laurifolius), Cistus parviflorus L. (C. parviflorus) and Cistus salviifolius L. (C. salviifolius). In this study, antiviral activity of 5 Cistus extracts were investigated against Infectious bronchitis virus (IBV) using the in ovo antiviral activity assay, which measures both embryo viability and agglutination of chicken red blood cells. In further experiments, antiviral potential of C. creticus extract was also examined against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-infected Vero-E6 cells. In ovo antiviral activity assays displayed that C. parviflorus and C. monspeliensis extracts caused more antiviral activity against IBV-D274 strain than other extracts. Further asssays showed that C. creticus extract led to dose and time dependent antiviral activity against SARS-CoV-2. The results proposed that C. parviflorus and C. monspeliensis extracts might have a potential for the treatment of virus-mediated diseases. Increasing incubation time and concentration of C. creticus extract led to increase of antiviral activity against SARS-CoV-2, which means that C. creticus extract had potent antiviral activity.

Keywords

Cistus L., IBV, SARS‑CoV‑2, Antiviral

Ethical Statement

On behalf of all authors, I hereby state that the work described here has not been published previously, that it is not under consideration for publication elsewhere, that its publication is approved by all Authors and tacitly or explicitly by the responsible authorities where the work was carried out, and that, if accepted, it will not be published elsewhere in the same form, in English or any other language, without the written consent of the Publisher. And this manuscript has not been checked by a language editing service before submission. I hope that it will be acceptable to be published in Hacettepe University Journal of the Faculty of Pharmacy.

Supporting Institution

None

Project Number

None

Thanks

We gratefully thank Assoc. Prof. Dr. Hasan YILDIRIM who shared the coordinates for the correct collection of Cistus species and Dr. Erdinc OGUR from the Aegean Agricultural Research Institute, who assisted collect the right species.

Cistus L.'nin Enfeksiyöz Bronşitis Virüsü ve SARS-CoV-2'ye Karşı Antiviral Potansiyeli

Abstract

Cistus L. (Cistus), Türkiye'de Cistus creticus L. (C. creticus), Cistus monspeliensis L. (C. monspeliensis), Cistus laurifolius L. (C. laurifolius), Cistus parviflorus L. (C) olmak üzere 5 tür tarafından yayılış göstermektedir. . parviflorus) ve Cistus salviifolius L. (C. salviifolius). Bu çalışmada, 5 Cistus ekstraktının antiviral aktivitesi, hem embriyo canlılığını hem de tavuk kırmızı kan hücrelerinin aglütinasyonunu ölçen in ovo antiviral aktivite tahlili kullanılarak Enfeksiyöz bronşitis virüsüne (IBV) karşı araştırıldı. Daha sonraki deneylerde, C. creticus ekstraktının antiviral potansiyeli, şiddetli akut solunum sendromu koronavirüs 2 (SARS-CoV-2) ile enfekte Vero-E6 hücrelerine karşı da incelenmiştir. İn ovo antiviral aktivite analizleri, C. parviflorus ve C. monspeliensis ekstraktlarının IBV-D274 suşuna karşı diğer ekstraktlara göre daha fazla antiviral aktiviteye neden olduğunu göstermiştir. Daha ileri analizler, C. creticus ekstraktının SARS-CoV-2'ye karşı doza ve zamana bağlı antiviral aktiviteye yol açtığını gösterdi. Sonuçlar, C. parviflorus ve C. monspeliensis ekstraktlarının virüs kaynaklı hastalıkların tedavisinde potansiyele sahip olabileceğini öne sürdü. Artan inkübasyon süresi ve C. creticus ekstraktının konsantrasyonu SARS-CoV-2'ye karşı antiviral aktivitenin artmasına yol açtı, bu da C. creticus ekstraktının güçlü antiviral aktiviteye sahip olduğu anlamına geliyor.

Keywords

Cistus L., IBV, SARS‑CoV‑2, Antiviral

Project Number

None

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