Rotavirus and Enteric Adenovirus Detection Among Diarrheic Outpatients in a Tertiary Hospital

Abstract

Background:Rotavirus and enteric Adenovirus are the most common agents of acute gastroenteritis.It was aimed to determine the frequency of rotavirus and enteric adenovirus among  patients admitted to our hosp ital with acute gastroenteritis.

Method:A total of 4230 outpatients with acute gastroenteritis were enrolled in this study.Rotavirus and enteric Adenovirus antigens were detected in stool samples of the patients by immunochromatographic method (Rota-Adeno Virus Combo Rapid Test, DIMA GmbH, Germany). Clinical data were obtained from patient records retrospectively.

Results:Rotavirus antigen was detected in 426 (10,1%) stool sample.Of the Rotavirus-infected patients, 297 (69,7%) were younger than 2 years.There was a significant differe nce(p<0.05) between patients younger than 2 years and those older than 2 years. Among Rotavirus-infected patients, 279(65,5%) were male and 147 (34,5%) were female.There was no significant difference (p>0.05) between genders in terms of rotavirus infection. Enteric Adenovirus antigen was detected in stool samples of 170(4%) patients. Of the Adenovirus-infected patents, 96(56,5%) were male, 74(43,5%)were female and 88(51,8%) were younger than 2 yearsold. There was no significant difference between genders and ages.

Conclusion:Rotavirus should be considered in diarrhea of children under 2 years. Rapid and properly diagnosis of viral gastroenteritis is crucial in prediction of clinical implications and reduction of unnecessary antibiotic usage.

Keywords

• Rotavirus,Enteric Adenovirus

References

1. 1. Chow CM, Leung AK, Hon KL. Acute gastroenteritis: from guidelines to real life. Clin Exp Gastroenterol 2010;3:97–112.
2. 2. Rodriguez-Baez N, O’Brien R, Qiu S-Q, Bass DM. Astrovirus, Adenovirus, and Rotavirus in Hospitalized Childr... : Journal of Pediatric Gastroenterology and Nutrition. J Pediatr Gastroenterol Nutr 2002;35(1):64–68.
3. 3. Winn WJ, Allen S, Janda W, et al. Diagnosis of Infections Caused by Viruses, Chlamydia, Rickettsia and Related Organisms [Internet]. In: Koneman’s Color Atlas and Textbook of Diagnostic Microbiology . Baltimore: Lippincott Williams &Wilkins; 2006. p. 1338.
4. 4. Bresee J, Fang Z-Y, Wang B, et al. First report from the Asian Rotavirus Surveillance Network. Emerg Infect Dis 2004;10(6):988–995.
5. 5. Parashar UD, Gibson CJ, Bresee JS, Glass RI. Rotavirus and severe childhood diarrhea. Emerg Infect Dis 2006;12(2):304–306.
6. 6. Parashar UD, Hummelman EG, Bresee JS, Miller MA, Glass RI. Global Illness and Deaths Caused by Rotavirus Disease in hildren. Emerg Infect Dis 2003;9(5):565–572.
7. 7. Tayeb HT, Balkhy HH, Aljuhani SM, Elbanyan E, Alalola S, Alshaalan M. Increased prevalence of rotavirus among children associated gastroen teritis in Riyadh Saudi Arabia. Virol J2011;8(1):548.
8. 8. Arnold A, MacMahon E. Adenovirus infections. In: LK P, editor. Medicine (United Kingdom). 2017. p. 777–780.

9. 9. Winn WJ, Allen S, Janda W, et al. Clinical Manifestations of Viral Infections. In: Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. Baltimore: Lippincott Williams &Wilkins; 2006. p. 1349.
10. 10. Ramsay M, Brown D. Epidemiology of group A rotaviruses. In: Gray J, Desselberger U, editors. Rotaviruses : methods and protocols. Totowa: Humana Press; 2000. p. 217–236.
11. 11. Roman E, Wilhelmi I, Colomina J, et al. Acute viral gastroenteritis: proportion and clinical relevance of multiple infections in Spanish children. J Med Microbiol 2003;52(5):435–440.
12. 12. Sánchez-Fauquier A, Montero V, Moreno S, et al. Human rotavirus G9 and G3 as major cause of diarrhea in hospitalized children, Spain. Emerg Infect Dis 2006;12(10):1536–1541.
13. 13. Temu A, Kamugisha E, Mwizamholya DL, Hokororo A, Seni J, Mshana SE. Prevalence and factors associated with Group A rotavirus infection among children with acute diarrhea in Mwanza, Tanzania. J Infect Dev Ctries 2012;6(6):508–515.
14. 14. González GG, Liprandi F, Ludert JE. Molecular epidemiology of enteric viruses in children with sporadic gastroenteritis in Valencia, Venezuela. J Med Virol 2011;83(11):1972–1982.
15. 15. Tüzüner U, Saran Gülcen B, Özdemir M, Feyzioğlu B. Gastroenteritli Çocukların Dışkılarında Adenovirus ve Rotavirus Sıklığı ve Mevsimsel Dağılımı Frequency of Adenovirus and Rotavirus and Their Seasonal Distribution in Children With Gastroenteritis. Klimik Derg 2016;29(3):121–124.
16. 16. Gül M, Garrpardiç M, Çiraggl P, et al. 0-5 Yaş Arası Gastroenteritli Çocuklarda Rotavirus ve Adenovirus Tip 40/41 Araştırılması. ANKEM Derg 2005;19(2):64–67.
17. 17. Biçer S, Tunca Ahin G, Koncay B, et al. Incidence assessment of rotavirus and adenovirus associated acute gastroenteritis cases in early childhood Incidenza di gastroenterite acuta da rotavirus e adenovirus nella prima infanzia. Le Infez Med 2011;2:113–119.
18. 18. Rad AY, Gözalan A. Detection of rotavirus and enteric adenovirus antigens in outpatients with gastroenteritis. Türkiye Klin tıp Bilim Derg 2010;30(1):174–179.
19. 19. Bayraktar B, Toksoy B, Bulut E. Akut Gastroenteritli Çocuklarda Rotavirus ve Adenovirus Saptanması Detection of Rotavirus and Adenovirus in Children with Acute Gastroenteritis. Klimik Derg 2010;23(1):15–17.
20. 20. Oh D-Y, Gaedicke G, Schreier E. Viral agents of acute gastroenteritis in German children: Prevalence and molecular diversi ty. J Med Virol 2003;71(1):82–93