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Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study

Year 2013, Volume: 5 Issue: 1, 9 - 15, 01.06.2013

Abstract

Background: Preeclampsia is characterized by hypertension and proteinuria occuring after the 20th week of
gestation. The purpose of this study was to compare the ultrastructural changes occuring in platelets of
preeclamptic and normotensive pregnant women.
Method: Venous blood samples of whom 20 with preeclampsia and 20 of normotensive, for platelets
extraction were firstly centrifuged. After prefixation and postfixation, was dehydrated at the increased
alcohol degrees, left in infiltration and blocked. Then, semi-thin sections taken with ultramicrotome were
stained with toludine blue. The assesment was made through light microscope following thin sections being
taken and stained with contrast, platelets were examined ultrastructurally and their electron micrographs
were taken.
Results: In the preeclampsia group the number of alpha and dense granules decreased, to a great extent,
and the structures of channel systems in tubular structure, were observed to be irregular.
Conclusion: It is clearly seen that preeclampsia induces cellular damage in platelets and in case of
aggravation in preeclampsia, it may cause significant changes in cell membranes of platelets and
cytoplasmic organelles.

References

  • Melanie McCabe White, Lisa K. Jennings Platelet protocols. Research and Clinical Laboratory Procedures. USA, Academic Press 1999.
  • Bizzozero G. Uber einen neuen Formbestandteil des Blutes und dessen Rolle bei der Thrombose und der Blutgerinnung. Virchows Arch Pathol Anat Physiol Klin Med 1881; 90: 216–284.
  • Hayem G. Sur le mechanism de’arret des hemorrhagies. C R Acad Sci 1878; 95:18–20.
  • Gerrard JM. Platelet aggregation: Cellular regulation and physiologic role. Hosp Pract 1998; 23:89–108.
  • Page CP. The involvement of platelets in nonthrombotic processes. Trends Pharmacol Sci 1988;9:66–71.
  • Roccella E. Report of the National High Blood Pressure Education Program Working Group on High blood pressure in pregnancy. Am J Obstet Gynecol, 2000;183:1-22.
  • Sankaralingam S, Arenas I, Lalu M et al. Preeclampsia: current understanding of the molecular basis of vascular dysfunction. Molecular medicine, 2006;3:1-20.
  • Sibai B. Diagnosis, prevention, and management of eclampsia. Obstet Gynecol, 2005; 105: 402–10.
  • Dekker G, Sibai B. Etiology and pathogenesis of preeclampsia: Current concepts. Am J Obstet Gynecol, 1998;179:1359-75.
  • Ballegeer V, Spitz B, de Baene L et al. Platelet activation and vascular damage in gestational hypertension. Am J Obstet Gynecol, 1992;166:629-33.
  • Weiss HJ. Platelet physiology and abnormalities of platelet function (first of two parts). N Engl J Med 1975;293:531– 541.
  • Richard C. Becker. Platelet Biology
 The Role of Platelets in Hemostasis, Thrombosis and Inflammation. Platelets in Cardiovascular Disease. Deepak L. Bhatt, USA, Cleveland Clinic,2007.1-36
  • Stenberg PE, Shuman MA, Levine SP et al. Redistribution of alpha granules and their contents in thrombin- stimulated platelets. J Cell Biol 1984;98:748–760.
  • Ginsberg MH, Taylor L, Painter RG. The mechanism of thrombin-induced platelet factor 4 secretion. Blood 1980;55:661–668.
  • Dolberg O, Ellis M. Successful Pregnancy and Delivery in a Woman with Gray Platelet Syndrome. Department of Medicine A, 2011; 13: 117–118.
  • James B. Buseel, Thomas J.Kunicki, Alan D. Michelson. Platelets: New Understanding of Platelets Glycoproteins and Their Role in Disease. Hematology Am Soc Hematolo Educ Program 2000, 222-240
  • Bercowitz K, Monteagudo A, Marks F. Mitochondrial Myopathy and Preeclampsia Associated with Pregnancy. Am J Obstet Gynecol, 1990, 162:146-147.
  • Pujol-Moıx N, Hernández A, Escolar G et al. Platelet ultrastructural morphometry for diagnosis of partial δ–storage pool disease in patients with mild platelet dysfunction and/or thrombocytopenia of unknown origin. A Study of 24 cases. Haematologica, 2000; 85:619 626.
  • Ahmed Y, Sullivan M, Elder M. Detection platelet clesensitization in pregnancy induced hypertension is dependent on the agonist used. Thromb Haemost, 65: 474-7, 1991.
  • Wang Y, Walsh S, Kay H. Placental tissue levels of nonesterified polyunsaturated fatty acids in normal and preeclamptic pregnancies. Hypertens Pregnancy, 2005;24:235-45.
  • Weinstein L. Syndrome of hemolysis, elevated liver enzymes, and low platelet count: a severe consequence of hypertension in pregnancy. Am J Obstet Gynecol, 1982;142:159-67.
Year 2013, Volume: 5 Issue: 1, 9 - 15, 01.06.2013

Abstract

References

  • Melanie McCabe White, Lisa K. Jennings Platelet protocols. Research and Clinical Laboratory Procedures. USA, Academic Press 1999.
  • Bizzozero G. Uber einen neuen Formbestandteil des Blutes und dessen Rolle bei der Thrombose und der Blutgerinnung. Virchows Arch Pathol Anat Physiol Klin Med 1881; 90: 216–284.
  • Hayem G. Sur le mechanism de’arret des hemorrhagies. C R Acad Sci 1878; 95:18–20.
  • Gerrard JM. Platelet aggregation: Cellular regulation and physiologic role. Hosp Pract 1998; 23:89–108.
  • Page CP. The involvement of platelets in nonthrombotic processes. Trends Pharmacol Sci 1988;9:66–71.
  • Roccella E. Report of the National High Blood Pressure Education Program Working Group on High blood pressure in pregnancy. Am J Obstet Gynecol, 2000;183:1-22.
  • Sankaralingam S, Arenas I, Lalu M et al. Preeclampsia: current understanding of the molecular basis of vascular dysfunction. Molecular medicine, 2006;3:1-20.
  • Sibai B. Diagnosis, prevention, and management of eclampsia. Obstet Gynecol, 2005; 105: 402–10.
  • Dekker G, Sibai B. Etiology and pathogenesis of preeclampsia: Current concepts. Am J Obstet Gynecol, 1998;179:1359-75.
  • Ballegeer V, Spitz B, de Baene L et al. Platelet activation and vascular damage in gestational hypertension. Am J Obstet Gynecol, 1992;166:629-33.
  • Weiss HJ. Platelet physiology and abnormalities of platelet function (first of two parts). N Engl J Med 1975;293:531– 541.
  • Richard C. Becker. Platelet Biology
 The Role of Platelets in Hemostasis, Thrombosis and Inflammation. Platelets in Cardiovascular Disease. Deepak L. Bhatt, USA, Cleveland Clinic,2007.1-36
  • Stenberg PE, Shuman MA, Levine SP et al. Redistribution of alpha granules and their contents in thrombin- stimulated platelets. J Cell Biol 1984;98:748–760.
  • Ginsberg MH, Taylor L, Painter RG. The mechanism of thrombin-induced platelet factor 4 secretion. Blood 1980;55:661–668.
  • Dolberg O, Ellis M. Successful Pregnancy and Delivery in a Woman with Gray Platelet Syndrome. Department of Medicine A, 2011; 13: 117–118.
  • James B. Buseel, Thomas J.Kunicki, Alan D. Michelson. Platelets: New Understanding of Platelets Glycoproteins and Their Role in Disease. Hematology Am Soc Hematolo Educ Program 2000, 222-240
  • Bercowitz K, Monteagudo A, Marks F. Mitochondrial Myopathy and Preeclampsia Associated with Pregnancy. Am J Obstet Gynecol, 1990, 162:146-147.
  • Pujol-Moıx N, Hernández A, Escolar G et al. Platelet ultrastructural morphometry for diagnosis of partial δ–storage pool disease in patients with mild platelet dysfunction and/or thrombocytopenia of unknown origin. A Study of 24 cases. Haematologica, 2000; 85:619 626.
  • Ahmed Y, Sullivan M, Elder M. Detection platelet clesensitization in pregnancy induced hypertension is dependent on the agonist used. Thromb Haemost, 65: 474-7, 1991.
  • Wang Y, Walsh S, Kay H. Placental tissue levels of nonesterified polyunsaturated fatty acids in normal and preeclamptic pregnancies. Hypertens Pregnancy, 2005;24:235-45.
  • Weinstein L. Syndrome of hemolysis, elevated liver enzymes, and low platelet count: a severe consequence of hypertension in pregnancy. Am J Obstet Gynecol, 1982;142:159-67.
There are 21 citations in total.

Details

Other ID JA34ZF46TS
Journal Section Research
Authors

Elif Erdem This is me

Murat Akkus This is me

Selcuk Tunik This is me

Yusuf Nergiz This is me

Mehmet Siddik Evsen This is me

Mehmet Zeki Taner This is me

Publication Date June 1, 2013
Published in Issue Year 2013 Volume: 5 Issue: 1

Cite

APA Erdem, E., Akkus, M., Tunik, S., Nergiz, Y., et al. (2013). Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study. International Archives of Medical Research, 5(1), 9-15.
AMA Erdem E, Akkus M, Tunik S, Nergiz Y, Evsen MS, Taner MZ. Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study. IAMR. June 2013;5(1):9-15.
Chicago Erdem, Elif, Murat Akkus, Selcuk Tunik, Yusuf Nergiz, Mehmet Siddik Evsen, and Mehmet Zeki Taner. “Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study”. International Archives of Medical Research 5, no. 1 (June 2013): 9-15.
EndNote Erdem E, Akkus M, Tunik S, Nergiz Y, Evsen MS, Taner MZ (June 1, 2013) Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study. International Archives of Medical Research 5 1 9–15.
IEEE E. Erdem, M. Akkus, S. Tunik, Y. Nergiz, M. S. Evsen, and M. Z. Taner, “Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study”, IAMR, vol. 5, no. 1, pp. 9–15, 2013.
ISNAD Erdem, Elif et al. “Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study”. International Archives of Medical Research 5/1 (June 2013), 9-15.
JAMA Erdem E, Akkus M, Tunik S, Nergiz Y, Evsen MS, Taner MZ. Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study. IAMR. 2013;5:9–15.
MLA Erdem, Elif et al. “Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study”. International Archives of Medical Research, vol. 5, no. 1, 2013, pp. 9-15.
Vancouver Erdem E, Akkus M, Tunik S, Nergiz Y, Evsen MS, Taner MZ. Comparison of Maternal Platelets in Preeclampsia and Normotensive Pregnancies: An Ultrastructural Study. IAMR. 2013;5(1):9-15.

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