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Year 2020, Volume: 12 Issue: 2, 7 - 15, 27.12.2020

Abstract

References

  • 1. Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochem Pharmacol. 2003;65(7):1035-41.
  • 2. Dudeck O, Jordan O, Hoffmann KT, Okuducu AF, Tesmer K, Kreuzer-Nagy T, et al. Organic solvents as vehicles for precipitating liquid embolics: a comparative angiotoxicity study with superselective injections of swine rete mirabile. AJNR Am J Neuroradiol. 2006;27(9):1900-6.
  • 3. Jacob SW, Herschler R. Pharmacology of DMSO. Cryobiology. 1986;23(1):14-27.
  • 4. Bakar B, Kose EA, Sonal S, Alhan A, Kilinc K, Keskil IS. Evaluation of the neurotoxicity of DMSO infused into the carotid artery of rat. Injury. 2012;43(3):315-22.
  • 5. Nasrallah FA, Garner B, Ball GE, Rae C. Modulation of brain metabolism by very low concentrations of the commonly used drug delivery vehicle dimethyl sulfoxide (DMSO). J Neurosci Res. 2008;86(1):208-14.
  • 6. Chaloupka JC, Vinuela F, Vinters HV, Robert J. Technical feasibility and histopathologic studies of ethylene vinyl copolymer (EVAL) using a swine endovascular embolization model. AJNR Am J Neuroradiol. 1994;15(6):1107-15.
  • 7. Chaloupka JC, Huddle DC, Alderman J, Fink S, Hammond R, Vinters HV. A reexamination of the angiotoxicity of selective injection of DMSO in the swine rete embolization model. AJNR Am J Neuroradiol. 1999;20(3):401-10.
  • 8. Ashwood-Smith MJ. Radioprotective and cryoprotective properties of dimethyl sulfoxide in cellular systems. Ann N Y Acad Sci. 1967;141(1):45-62.
  • 9. Jacob SW, Wood DC. Dimethyl sulfoxide (DMSO). Toxicology, pharmacology, and clinical experience. Am J Surg. 1967;114(3):414-26.
  • 10. Sams WM, Jr., Carroll NV. Cholinesterase inhibitory property of dimethyl sulphoxide. Nature. 1966;212(5060):405.
  • 11. Archer JM, Shilkin KB, Papadimitriou JM, Walters MN. Interaction of dimethylsulfoxide and ascorbic acid on fibroblasts of rats. Proc Soc Exp Biol Med. 1967;126(2):354-6.
  • 12. Hervas JP, Gimenez-Martin G. Dimethyl sulphoxide effect on division cells. Experientia. 1973;29(12):1540-2.
  • 13. A I. Tamiflu’nun insan periferal lenfositlerinde in vitro genotoksik ve sitotoksik etkileri.: Cukurova Üniversity; 2009.
  • 14. Rencüzoğulları E, Topaktaş MJFvMBD. The relationship between quantities of bromodeoxyuridine and human peripheral blood with determination of the best differential staining of sister chromatids using chromosome medium-B. 1991;5(3):19-24.
  • 15. N B. Tıbbi Genetik. 7 ed: Nobel-İstanbul; 1999.
  • 16. G L. Sitogenetiğin Temelleri: Meteksan-Ankara; 1990.

The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes

Year 2020, Volume: 12 Issue: 2, 7 - 15, 27.12.2020

Abstract

Objective: The main aim of this study was to examine the effects of dimethyl sulfoxide (DMSO) on chromosomal abnormalities in human peripheral blood lymphocytes.
Methods: Peripheral blood samples were collected from two healthy men and two healthy women. Then, in vitro studies were conducted with these blood samples , and the results were cytogenetically analyzed .
There were two groups: a DMSO group and a control group.
DMSO and control medium were added to the samples at 24 hours and 48 hours.
Results: A total of 800 metaphases were examined in this study. Depending on the increase in the number of groups and the time of application, an increase in chromosomal abnormalities was observed, and these were recorded.
Conclusion: In many previous studies, the effects of DMSO have been examined in various tissues and the body, but there are fewer studies about the effects on chromosomes. In this study, we researched the effects of DMSO, except for negative effects and toxicity, on chromosomal abnormalities.

References

  • 1. Santos NC, Figueira-Coelho J, Martins-Silva J, Saldanha C. Multidisciplinary utilization of dimethyl sulfoxide: pharmacological, cellular, and molecular aspects. Biochem Pharmacol. 2003;65(7):1035-41.
  • 2. Dudeck O, Jordan O, Hoffmann KT, Okuducu AF, Tesmer K, Kreuzer-Nagy T, et al. Organic solvents as vehicles for precipitating liquid embolics: a comparative angiotoxicity study with superselective injections of swine rete mirabile. AJNR Am J Neuroradiol. 2006;27(9):1900-6.
  • 3. Jacob SW, Herschler R. Pharmacology of DMSO. Cryobiology. 1986;23(1):14-27.
  • 4. Bakar B, Kose EA, Sonal S, Alhan A, Kilinc K, Keskil IS. Evaluation of the neurotoxicity of DMSO infused into the carotid artery of rat. Injury. 2012;43(3):315-22.
  • 5. Nasrallah FA, Garner B, Ball GE, Rae C. Modulation of brain metabolism by very low concentrations of the commonly used drug delivery vehicle dimethyl sulfoxide (DMSO). J Neurosci Res. 2008;86(1):208-14.
  • 6. Chaloupka JC, Vinuela F, Vinters HV, Robert J. Technical feasibility and histopathologic studies of ethylene vinyl copolymer (EVAL) using a swine endovascular embolization model. AJNR Am J Neuroradiol. 1994;15(6):1107-15.
  • 7. Chaloupka JC, Huddle DC, Alderman J, Fink S, Hammond R, Vinters HV. A reexamination of the angiotoxicity of selective injection of DMSO in the swine rete embolization model. AJNR Am J Neuroradiol. 1999;20(3):401-10.
  • 8. Ashwood-Smith MJ. Radioprotective and cryoprotective properties of dimethyl sulfoxide in cellular systems. Ann N Y Acad Sci. 1967;141(1):45-62.
  • 9. Jacob SW, Wood DC. Dimethyl sulfoxide (DMSO). Toxicology, pharmacology, and clinical experience. Am J Surg. 1967;114(3):414-26.
  • 10. Sams WM, Jr., Carroll NV. Cholinesterase inhibitory property of dimethyl sulphoxide. Nature. 1966;212(5060):405.
  • 11. Archer JM, Shilkin KB, Papadimitriou JM, Walters MN. Interaction of dimethylsulfoxide and ascorbic acid on fibroblasts of rats. Proc Soc Exp Biol Med. 1967;126(2):354-6.
  • 12. Hervas JP, Gimenez-Martin G. Dimethyl sulphoxide effect on division cells. Experientia. 1973;29(12):1540-2.
  • 13. A I. Tamiflu’nun insan periferal lenfositlerinde in vitro genotoksik ve sitotoksik etkileri.: Cukurova Üniversity; 2009.
  • 14. Rencüzoğulları E, Topaktaş MJFvMBD. The relationship between quantities of bromodeoxyuridine and human peripheral blood with determination of the best differential staining of sister chromatids using chromosome medium-B. 1991;5(3):19-24.
  • 15. N B. Tıbbi Genetik. 7 ed: Nobel-İstanbul; 1999.
  • 16. G L. Sitogenetiğin Temelleri: Meteksan-Ankara; 1990.
There are 16 citations in total.

Details

Primary Language English
Subjects ​Internal Diseases
Journal Section Research
Authors

İlyas Yücel 0000-0002-4446-0469

Mahir Binici 0000-0003-1039-9361

Fikriye Fulya Kavak 0000-0003-0278-7188

Diclehan Oral 0000-0002-0074-0602

Selahattin Tekeş

Mahmut Balkan 0000-0003-0138-6622

Publication Date December 27, 2020
Published in Issue Year 2020 Volume: 12 Issue: 2

Cite

APA Yücel, İ., Binici, M., Kavak, F. F., Oral, D., et al. (2020). The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes. International Archives of Medical Research, 12(2), 7-15.
AMA Yücel İ, Binici M, Kavak FF, Oral D, Tekeş S, Balkan M. The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes. IAMR. December 2020;12(2):7-15.
Chicago Yücel, İlyas, Mahir Binici, Fikriye Fulya Kavak, Diclehan Oral, Selahattin Tekeş, and Mahmut Balkan. “The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes”. International Archives of Medical Research 12, no. 2 (December 2020): 7-15.
EndNote Yücel İ, Binici M, Kavak FF, Oral D, Tekeş S, Balkan M (December 1, 2020) The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes. International Archives of Medical Research 12 2 7–15.
IEEE İ. Yücel, M. Binici, F. F. Kavak, D. Oral, S. Tekeş, and M. Balkan, “The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes”, IAMR, vol. 12, no. 2, pp. 7–15, 2020.
ISNAD Yücel, İlyas et al. “The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes”. International Archives of Medical Research 12/2 (December 2020), 7-15.
JAMA Yücel İ, Binici M, Kavak FF, Oral D, Tekeş S, Balkan M. The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes. IAMR. 2020;12:7–15.
MLA Yücel, İlyas et al. “The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes”. International Archives of Medical Research, vol. 12, no. 2, 2020, pp. 7-15.
Vancouver Yücel İ, Binici M, Kavak FF, Oral D, Tekeş S, Balkan M. The Effect of Dimethyl Sulfoxide on Chromosomal Abnormalities in Human Peripheral Blood Lymphocytes. IAMR. 2020;12(2):7-15.

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