Electrophoretic characterization of inbred maize lines

Abstract

In this study, 50 inbred lines (S₄) of maize (Zea mays indentata Sturt.), which were developed by the Department of Field Crops, Faculty of Agriculture, University of Namık Kemal, were used as a material.  In the study, the band patterns of the gliadin protein of inbred lines were determined by the SDS – PAGE method, and regarding the ratio density data of the genotypes, the number of the bands and the spreading of them to the gliadin regions were examined. At the end of the electrophoresis examinations, it was revealed that the band number of the gliadin proteins in the inbred maize lines was between 11 and 20, the relative mobility of the genotypes was between 18 and 90 kDA, and according to the gliadin regions, the bands were mainly in the omega, beta and gamma regions, respectively. It was found out that the relative mobility was minimum in the alpha region. As a result of the study, it was determined that some lines were formed by similar populations with the obtained band patterns, and the majority were different. This indicates that genetic diversity exists in the examined lines, and the obtained data can be used in the breeding studies.

Keywords

• Zea mays L.,Inbred line,SDS-PAGE,Gliadin electrophoresis,Electrophoretic character

References

1. 1. Ranere, A.J., Piperno, D.R., Holst, I., Dickau, R., Iriarte, J. The cultural and chronological context of early Holocene maize and squash domestication in the Central Balsas River Valley, Mexico. Proc Natl Acad Sci, 2009. 106: 5014–5018.
2. 2. FAO Internet Web Site, FAOSTAT. Database. [cited 2017 1 August]; Available from: www.fao.org
3. 3. Ziraat Mühendisleri Odası, [cited 2016 10 August]; Available from: http://www.zmo.org.tr/genel
4. 4. 4. Cengiz, R. Türkiye’de Kamu Mısır Araştırmaları [Public Maize Investigations in Turkey]. Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi, 2016. 25: 304-310.
5. 5. USDA/FAS, cited 2015 21 January]; Available from: http://www.fas.usda.gov/search/Corn%20production
6. 6. Pingali, P.L. CIMMYT 1999-2000, World Maize Facts and Trends. Meeting World Maize Needs: Technological Opportunities and Priorities for the Public Sector. 2001. Mexico, D.F.: CIMMYT.
7. 7. Osborne, T. B., The amount and properties of the proteids of the maize kernel. J. Amer. Chem. Soc, 1897. 19: 525-532.
8. 8. Dierks-Ventling, C., Cozens, K., Immunochemical Crossreactivity Between Zein, Hordein And Gliadin. Herbs Letters, 1982. 14:147-150.

9. 9. Valentini, G., Soave, C., Ottaviano, E., Chromosomal location of zein genes in Zea mays. Heredity, 1979. 42:33–40.
10. 10. Esen, A., A proposed nomenclature for the alcohol-soluble proteins (zeins) of maize (Zea mays L.). Journal of Cereal Science, 1987. 5:117-128.
11. 11. Holding, D.R., Larkins, B.A. Zein storage proteins. In Molecular Genetic Approaches to Maize Improvement, AL KrizBA Larkins, Biotechnology in Agriculture and Forestry, Springer- Verlag Berlin Heidelberg, Germany, 2009. 63: 269–286.
12. 12. Krishnan, H.B., Kerley, M.S., Allee, G.L., Jang, S., Kim, W.S. et al. Maize 27 kDa γ-Zein Is a Potential Allergen for Early Weaned Pigs. J Agric Food Chem, 2010. 58: 7323–7328.
13. 13. Șuteu, D., Băcilă, I., Haș,V., Haș, I., Miclăuș, I., Romanian Maize (Zea mays) Inbred Lines as a Source of Genetic Diversity in SE Europe, and Their Potential in Future Breeding Efforts. Plos One, 2013. 8:1-13.
14. 14. Lookhart, G. and Bean, S., Separation and characterization of wheat protein fractions by high-performance capillary electrophoresis. Cereal Chem., 1995. 72: 527–532.
15. 15. Đukić, N., Matić, G., Konjević, R. Biochemical Analysis Of Gliadins Of Wheat Triticum. Kragujevac J. Sci., 2005. 27:131-138.
16. 16. Waga, J. and Zientarski, J., Isolation And Purification Of Individual Gliadin Proteins by Preparative Acid Polyacrylamide Gel Electrophoresis (A-Page). Polish Journal Of Food And Nutrition Sciences, 2007. 57:91-96.
17. 17. Žilić, S., Barać, M., Pešić, M., Dodig, D., Ignjatović-Micić, D.,Characterization Of Proteins From Grain of Different Bread and Durum Wheat Genotypes. International Journal of Molecular Sciences, 2011. 12:5878-5894.
18. 18. Xu, J.H. and Messing, J., Organization of the prolamin gene family provides insight into the evolution of the maize genome and gene duplications in grass species. Proc Natl Acad Sci of the USA, 2008. 105: 14330–14335.
19. 19. Swarup, S., Timmermans, M.C., Chaudhuri, S., Messing, J., Determinants of the high-methionine trait in wild and exotic germplasm may have escaped selection during early cultivation of maize. Plant J, 1995. 8: 359–368.
20. 20. Wang, L., Xu, C., Qu, M., Zhang, J., Kernel amino acid composition and protein content of introgression lines from Zea mays ssp. Mexicana into cultivated maize. Journal of Cereal Science, 2008. 48: 387–393.
21. 21. Kosmolak, F. G. et al., A relationship between durum wheat quality and gliadin electrophorograms. Ca. J. Plant Sci., 1980. 60: 427- 432.
22. 22. Bushuk, W. and Zillman, R.R., Wheat cultivar identification by gliadin electroforegrams. I. Apparatus, method and nomenclature. Can. J. Plant Sci., 1978.58: 505-515.
23. 23. Motel, J. S. et al., Zentral Institüt für Genetik und Kulturpflanzenforshung der Akademie der Wissenschaften der DDR, Corrensstrasse 3. 434 Gatersleben, DDR. 1981.
24. 24. Lookhart et al., An improved method of standardizing polyacrylamide gel electrophoresis of wheat gliadin Proteins, Cereal Chem., 1982. 59: 178-181.
25. 25. Güney, E., Tan, M., Dumlugül, Z., Gül, İ. Erzurum Şartlarında Bazı Silajlık Mısır Çeşitlerinin Verim ve Silaj Kalitelerinin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 2011. 41:105-111.
26. 26. Özata, E., Öz, A., Kapar, H. Silajlık Hibrit Mısır Çeşit Adaylarının Verim ve Kalite Özelliklerinin Belirlenmesi. Tarım Bilimleri Araştırma Dergisi, 2012. 5: 37-41.
27. 27. Gil-Humanes, J., Pisto,´F., Altamirano-Fortoul, R., Real, A., Comino, I., Sousa, C., Rosell, C.M., Barro, F. Reduced-Gliadin Wheat Bread: An Alternative to the Gluten-Free Diet for Consumers Suffering Gluten-Related Pathologies. Plos One, 2014, 9 : 1-9.
28. 28. Wenefrida, I., Utomo, H.S., Blanche,S.B., Linscombe, S.D., Enhancing essential amino acids and health benefit components in grain crops for improved nutritional values. Recent patents on DNA & gene sequences, 2009. 3: 219–225.
29. 29. Glover, D.V. Corn protein-genetics, breeding, and value in foods and feeds. In: Mertz ET, editor. Quality Protein Maize. St. Paul, MN: American Association of Cereal Chemist, 1992. 49–78.
30. 30. Wu, X.R., Kenzior, A., Willmot, D., Scanlon, S., Chen, Z., et al. Altered expression of plant lysyl tRNA synthetase promotes tRNA misacylation and translational recoding of lysine. Plant J., 2007. 50: 627–636.