ANJİYOJENİK VE ANTİANJİYOJENİK ÖZELLİĞİ BELİRLEMEDE KULLANILAN in vitro ve in vivo YÖNTEMLER

Year 2019, Volume: 2 Issue: 3, 141 - 159, 29.12.2019

Gönül Ulus

Abstract

Anjiyogenez damar ağlarından yeni kan damarlarının gelişimidir ve yara iyileşmesi, dişi üreme sistemi, fetal gelişim gibi fizyolojik durumlar; sedef hastalığı, diabetik retinopati, tümör gelişimi ve metestaz gibi patolojik durumlar için gerekli bir süreçtir. Anjiyojenez analizleri ajanların anjiyojenik veya anti-anjiyojenik özellikleri belirlemek için kullanılmaktadır. Anjiyogenik aktivite in vitro ve in vivo yöntemler kullanılarak değerlendirilir. Bu yöntemlerin herbirinin avantaj ve dezavantajları bulunmaktadır. İdeal bir metot kolayca ölçülebilir, hassas ve güvenilir olmalıdır. Bu çalışmanın temel amacı, bu alanda çalışan araştırmacılara anjiyojenik karakteri belirlemek için mevcut olan yöntemleri tanıtmaktır.

Keywords

Angiogenesis assays, in vivo, in ovo, in vitro

METHODS FOR ASSAYING ANGIOGENIC-ANTIANGIOGENIC FEATURE in vitro and in vivo

Abstract

Angiogenesis is the growing of new blood vessels from vasculature network and essential in many conditions like wound healing, female reproductive system, fetal development as well as psoriasis, diabetic retinopathy, tumour growth and metastasis. Angiogenesis assays are maintained to determine the angiogenic or anti-angiogenic properties of the agents. Angiogenic activity can be determined by using in vitro and in vivo systems. These methods have advantages and disadvantages yet. Nevertheless an ideal method should be easily measurable, precise and reliable. The main objective of the review is introduce the methods available to determine the angiogenic character to the researchers working in this field.

Keywords

Angiogenesis assays, in vivo, in ovo, in vitro

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