An Investigation on the Effect of Nano-ZnO Application on Cadmium Phytoextraction by Safflower

Abstract

Nowadays, soils contaminated with heavy metals are one of the biggest environmental pollution problems in the world. The phytoextraction method is the most effective and well-known plant remediation method that can be used to clean up agricultural soils contaminated with heavy metals. Nanoparticle applications have recently been introduced to remove pollutants, promote plant growth and improve pollutant phyto-availability to improve the efficiency/effectiveness of this method. In this study, it is aimed to use phytoextraction method and nanomaterial together for the cleaning of cadmium (Cd) contaminated growth media and to investigate the effects of nanomaterial on plants. For this purpose, a hydroponic culture was planned and ZnO-NP, a nanomaterial, which was determined by OECD as a priority, was used for the experiment. As plant; drought-resistant, without climatic selectivity, can be grown in different ecological conditions, safflower (Carthamus tinctorius) (Dinçer variety) was selected. Safflower seeds were germinated in a mixture of peat-perlite (1:1), and after 2-3 leaves, they were transferred to the Hoagland nutrient solution. In order to see the effects of Cd × ZnO-NP applications, morphological observations of the plants were made and chlorophyll contents were measured before the harvest by applying ZnO-NP and (0-3-6 mg / L) Cd in increasing doses (0-5-10 mg / L) to the nutrient solution. Plants were harvested from plants grown for 20 days. Green parts and root dry weights of plants, Zn, and Cd concentrations were determined. The results showed that Cd accumulation of the plant increased due to increasing doses of ZnO-NP. In the green part of the safflower plant, Cd has accumulated 5.2 to 8.7 times more Cd than the hyperaccumulation critical threshold value (100 µg / g). The research showed that the Cd phytoremediation potential of the safflower plant was high.

Keywords

• Phytoextraction
• cadmium
• ZnO nanoparticle
• safflower
• hydroponic culture.

References

1. Angelova, V.R., Perifanova-Nemska, M.N., Uzunova, G.P., Kolentsova, E.N. (2016). Accumulation of heavy metals in safflower (Carthamus tinctorius L.), Int J Biol, Biomolecular, Agric, Food Biotechnological Eng., 10(7):410-415.
2. ATSDR, (2021), The ATSDR (2019). Substance Priority List, The Agency for Toxic Substances and Disease Registry (ATSDR) of the U.S. Department of Health and Human Services. URL Link: https://www.atsdr.cdc.gov/spl/index.html
3. Çağlarırmak, N., Hepçimen, A. Z. (2010). Ağır metal toprak kirliliğinin gıda zinciri ve insan sağlığına etkisi, Akademik Gıda, 8(2): 31-35.
4. Dağhan, H., Köleli, N., Uygur, V., Arslan, M., Önder, D., vd. (2012). Investigation of the use of transgenic tobacco plant in the treatment of cd-contaminated soils by phytoextraction, Soil Water Journal, 1:1-6.
5. Dağhan, H., and Öztürk, M. (2015). Soil pollution in Turkey and remediation methods. Soil remediation and plants: prospects and challenges, 287-312. Edited by Khalid Hakeem, Muhammad Sabir, Munir Ozturk, Ahmet Mermut, Academic Press, Elsevier.
6. Dağhan, H. (2018). Effects of TiO2 nanoparticles on maize (Zea mays l.) growth, chlorophyll content and nutrient uptake. Appl. Ecol. Environ. Res., 16:6873-6883.
7. Daghan H. (2019). Transgenic tobacco crops for phytoremediation metals and metalloids. Chapter 13, 279-297, In Transgenic Plant Technology Forremediation of Toxic Metals and Metalloids, Editor: Majeti Narasimha Vara Prasad. Publisher: Academic Press, Elsevier, London, United Kingdom. ISBN: 9780128143896. URL Link: https://www.elsevier.com/books/transgenic-plant-technology-for-remediation-of-toxic-metals-and-metalloids/prasad/978-0-12-814389-6.
8. Eren, A. and Baran, M.F. (2019). Green synthesis, characterization and antimicrobial activity of silver nanopartıcles (AgNPs) from maize (Zea mays L.), Appl. Ecol. Environ. Res., 17(2): 4097-4105.

9. Esetlili B. Ç., Anaç D. (2015). Ağır metal kirliliği ve fitoremediasyon, Apelasyon Dergisi, Mayıs 2015, Sayı: 18. September 2021 from http://apelasyon.com/Yazi/263-agir-metal-kirliligi-ve-fitoremediasyon
10. Gokhale, Y. P., Kumar, R., Kumar, J., Hintz, W., Warnecke, G., & Tomas, J. (2009). Disintegration process of surface stabilized sol-gel TiO2 nanoparticles by population balances. Chem. Eng. Sci., 64(24): 5302-5307
11. Gowayed, S. M. H. (2017). Impact of zinc oxide nanoparticles on germination and antioxidant system of maize (Zea mays L.) seedling under cadmium stress, J. Plant Prod. Sci., Suez Canal University, 6 (1): 1-11.
12. Hoagland D.R, Arnon D.I. (1950). The water culture method for growing plants without soil, The College of Agriculture, University of California, Calif. Agric. Exp. Stn., Circ., Berkeley, 347: 1-32.
13. Hua, M., Zhang, S., Pan, B., Zhang, W., Lv, L., & Zhang, Q. (2012). Heavy metal removal from water/wastewater by nanosized metal oxides: a review. J. Hazard. Mater., 211, 317-331.
14. Hussain, F., Hadi, F., & Rongliang, Q. (2021). Effects of zinc oxide nanoparticles on antioxidants, chlorophyll contents, and proline in Persicaria hydropiper L. and its potential for Pb phytoremediation. Environ. Sci. Pollut. Res., 1-17.
15. Kabata-Pendias, A. (2000). Trace elements in soils and plants. CRC press.
16. Keller C, Rizwan M, Davidian JC, Pokrovsky OS, Bovet N, Chaurand P, Meunier JD, (2015). Effect of silicon on wheat seedlings (Triticum turgidum L.) grown in hydroponics and exposed to 0 to 30 μM Cu. Planta 241:847–860
17. Khan, Z.S., Rizwan, M., Hafeez, M., Ali, S., Javed, M.R., vd. (2019). The accumulation of cadmium in wheat (Triticum aestivum) as influenced by zinc oxide nanoparticles and soil moisture conditions, Environ. Sci. Pollut. Res., 26(19): 19859-19870.
18. Khurana, M., Kansal, B. (2012). Influence of zinc supply on the phytotoxicity of cadmium in maize (Zea mays L.) grown on cadmium-contaminated soil, Acta Biol. Hung. 60(1): 37-46.
19. Köleli, N., Eker, S., Cakmak, I. (2004). Effect of zinc fertilization on cadmium toxicity in durum and bread wheat grown in zinc-deficient soil, Environ. Pollut., 131(3): 453-459.
20. Köleli N., Dağhan H., Demir A., Coşkuner Y., Doğaroğlu Z. G. (2018). Çoklu metalle (Cd, Pb ve Zn) kirlenmiş topraklarda yağlı tohumlu bitkilerin fitoremediasyon kapasitesinin araştırılması ve elde edilen biyokütlenin değerlendirilmesi, TÜBİTAK, Proje No: 115-Y-337 nolu proje Kesin Sonuç Raporu, s.120.
21. Marschner, H. (1995). Mineral nutrition of higher plants. Academic Press. Inc., San Diago.
22. Müftüoğlu, M.N., Türkmen, C.,and Çıkılı Y., (2012). Toprak ve bitkide verimlilik analizleri, Kriter Yayınevi, İstanbul, ISBN:978-605-4613-32-8.
23. OECD (2013). Organization for Economic Co-operation and Development (OECD), OECD Environment, Health and Safety Publications, Series on the Safety of Manufactured Nanomaterials, No.37, ENV/JM/MONO (2013) 2, September 2021 from http://search.oecd.org/officialdocuments/displaydocumentpdf/?cote=env/jm/mono(2013)2&doclanguage=en
24. Rizwan, M., Ali, S., Ali, B., Adrees, M., Arshad, M., Hussain, A., ur Rehman, M.Z. and Waris, A.A., (2019a). Zinc and iron oxide nanoparticles improved the plant growth and reduced the oxidative stress and cadmium concentration in wheat, Chemosphere, 214: 269-277.
25. Rizwan, M., Ali, S., ur Rehman, M.Z. and Maqbool, A. (2019b). A critical review on the effects of zinc at toxic levels of cadmium in plants, Environ. Sci. Pollut. Res., 26(7):6279-6289.
26. Seven, T., Can, B., Darende, B. N., and Ocak, S., (2018). Hava ve toprakta ağır metal kirliliği. Ulusal Çevre Bilimleri Araştırma Dergisi, 1(2), 91-103.
27. Song, B., Xu, P., Chen, M., Tang, W., Zeng, G., Gong, J., Zhang, P. & Ye, S. (2019). Using nanomaterials to facilitate the phytoremediation of contaminated soil. Crit. Rev. Environ. Sci. Technol., 49(9):791-824, DOI:10.1080/10643389.2018.1558891
28. Tang, W. W., Zeng, G. M., Gong, J. L., Liang, J., Xu, P., Zhang, C., and Huang, B. B. (2014). Impact of humic/fulvic acid on the removal of heavy metals from aqueous solutions using nanomaterials: a review. Sci. Total Environ., 468: 1014-1027.
29. Van der Ent, A., Baker, A.J.M., Reeves, R.D., Pollard, A.J., Schat, H. (2013). Hyperaccumulators of metal and metalloid trace elements: facts and fiction, Plant and Soil, 362: 319-334.
30. Venkatachalam, P., Jayaraj, M., Manikandan, R., Geetha, N., Rene, E.R., vd. (2017). Zinc oxide nanoparticles (ZnO-NP) alleviate heavy metal-induced toxicity in Leucaena leucocephala seedlings: a physiochemical analysis, Plant Physiol. Biochem., 110: 59-69.
31. Zhang, W., Long, J., Li, J., Zhang, M., Xiao, G., Ye, X., Chang, W. and Zeng, H., (2019). Impact of ZnO nanoparticles on Cd toxicity and bioaccumulation in rice (Oryza sativa L.), Environ. Sci. Pollut. Res., 26(22):23119-23128
32. Zhu, Y., Xu, F., Liu, Q., Chen, M., Liu, X., Wang, Y., Sun, Y. and Zhang, L., (2019). Nanomaterials and plants: Positive effects, toxicity and the remediation of metal and metalloid pollution in soil, Sci. Total Environ., 662:414-421.