Identificatıon and Genetic Diversity of Hypovirulent Binucleate Rhizoctonia spp. Isolated from Turfgrass and Soil

Abstract

The aim of this study was to obtain hypovirulent binucleate Rhizoctonia anastomosis groups from turfgrass roots and rhizosphere soils in Eskişehir, Ankara and Kocaeli. In 2022, 153 turfgrass and 78 rhizosphere soil were taken from turfgrass areas. Isolations were made from the roots and rhizosphere soils. Isolations from soils were performed by colonization of trap plant tissues. Detection of anastomosis groups of the isolates were done according to the hyphal nucleus staining, colony apparences and rDNA-ITS sequences analysis. The ITS regions of the isolates were amplified by polymerase chain reaction (PCR) using rDNA primer pair ITS 1 and ITS2. Pathogenicity tests of all isolates were conducted with pot assays in greenhouse conditions. As a result of the study, 17 BNR isolates belonging to 7 different anastomosis groups were obtained. Two AG D isolates were found pathogenic. As a result, 15 hypovirulent binucleate Rhizoctonia isolates belonging to AG A, AG B(o), AG Fa, AG Fb, AG G and AG K groups were obtained. The phylogenetic neighbor-joining tree of 17 Binucleate isolates clearly shows that the isolates are grouped into seven distinct clusters. AG Fa and AG Fb appeared on the tree as genetically close isolates. In future, studies should be conducted to investigate the effect levels of the hypovirulent strains detected in this study on the control of pathogenic fungi that cause diseases in cultivated plants.

Keywords

• anastomosis group
• turfgrass
• soil
• phylogeny

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