Viability and Germination of Wheat Pollen at Various Storage Temperatures

Bekir Atar; Sultan Filiz Güçlü

Viability and Germination of Wheat Pollen at Various Storage Temperatures

Abstract

Unlike many plant species, wheat pollen cannot maintain its viability for a long time. Extending the storage period of pollen is very important for technological breeding studies and conservation of genetic resources. A limited number of studies on this subject have not yet achieved sufficient success. In this study, pollen of seven wheat cultivars (registered and landraces) of different species were stored at three storage temperatures (room temperature, +4oC and -18oC) and three different storage periods (0, 48 and 96 hours) to determine pollen viability and germination rates. Spikes (when 40-80% of flowers were seen) were cut 20 cm down and taken to the laboratory and the tests were started by placing them in containers filled with pure water. Pollen viability rates were determined by TTC staining test and pollen germination rates were determined by ‘petride agar’ method. Statistically significant differences were found between varieties, storage temperatures and storage periods. In the first test, pollen viability and germination rates were 77% and 43%, respectively. After 48 hours of storage, pollen viability decreased to 44% at 4oC, 12% at -18oC and all pollen lost viability at room temperature. After 48 hours storage at 4oC, germination rate decreased to 22%. No germination was observed at 18oC and room temperature. In the fresh test, the highest viability and germination rate was determined in Esperia variety, the lowest viability rate was determined in Kocabugday and the lowest germination rate was determined in Ç-1252 variety. At 4oC for 48 hours, the highest viability and germination rate was determined in Esperia variety, the lowest viability rate was determined in Bezostaja-1 and the lowest germination rate was determined in Kocabugday variety. Although wheat pollen gives better results in storage at 4oC compared to storage at room temperature and -18oC, it completely loses its viability and germination potential after 4 days in all environments.

Keywords

References

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Details

Primary Language

English

Subjects

Agronomy

Journal Section

Research Article

Authors

Bekir Atar ^*
0000-0002-1446-5699
Türkiye

Sultan Filiz Güçlü
0000-0003-0561-7037
Türkiye

Early Pub Date

November 28, 2025

Publication Date

December 26, 2025

Submission Date

October 2, 2025

Acceptance Date

October 29, 2025

Published in Issue

Year 2025 Volume: 9 Number: 2

IZ

https://izlik.org/JA76CR34PP

Cite

RIS / Bibtex

APA

Atar, B., & Güçlü, S. F. (2025). Viability and Germination of Wheat Pollen at Various Storage Temperatures. International Journal of Agriculture Forestry and Life Sciences, 9(2), 104-110. https://izlik.org/JA76CR34PP

AMA

1.Atar B, Güçlü SF. Viability and Germination of Wheat Pollen at Various Storage Temperatures. Int J Agric For Life Sci. 2025;9(2):104-110. https://izlik.org/JA76CR34PP

Chicago

Atar, Bekir, and Sultan Filiz Güçlü. 2025. “Viability and Germination of Wheat Pollen at Various Storage Temperatures”. International Journal of Agriculture Forestry and Life Sciences 9 (2): 104-10. https://izlik.org/JA76CR34PP.

EndNote

Atar B, Güçlü SF (December 1, 2025) Viability and Germination of Wheat Pollen at Various Storage Temperatures. International Journal of Agriculture Forestry and Life Sciences 9 2 104–110.

IEEE

[1]B. Atar and S. F. Güçlü, “Viability and Germination of Wheat Pollen at Various Storage Temperatures”, Int J Agric For Life Sci, vol. 9, no. 2, pp. 104–110, Dec. 2025, [Online]. Available: https://izlik.org/JA76CR34PP

ISNAD

Atar, Bekir - Güçlü, Sultan Filiz. “Viability and Germination of Wheat Pollen at Various Storage Temperatures”. International Journal of Agriculture Forestry and Life Sciences 9/2 (December 1, 2025): 104-110. https://izlik.org/JA76CR34PP.

JAMA

1.Atar B, Güçlü SF. Viability and Germination of Wheat Pollen at Various Storage Temperatures. Int J Agric For Life Sci. 2025;9:104–110.

MLA

Atar, Bekir, and Sultan Filiz Güçlü. “Viability and Germination of Wheat Pollen at Various Storage Temperatures”. International Journal of Agriculture Forestry and Life Sciences, vol. 9, no. 2, Dec. 2025, pp. 104-10, https://izlik.org/JA76CR34PP.

Vancouver

1.Bekir Atar, Sultan Filiz Güçlü. Viability and Germination of Wheat Pollen at Various Storage Temperatures. Int J Agric For Life Sci [Internet]. 2025 Dec. 1;9(2):104-10. Available from: https://izlik.org/JA76CR34PP