Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers

Mücahit Furkan Yeşilbaş; Emel Sözen; Yasemin Evrenosoğlu

doi:10.66598/ijafls.1888824

Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers

Abstract

The quality of genomic DNA is a critical factor for the reliability of molecular analyses in clonal plant materials. In Prunus species, DNA isolation is often challenging due to the high content of polysaccharides and phenolic compounds in leaf tissues, which can interfere with downstream enzymatic reactions. In this study, a CTAB-based DNA isolation protocol was evaluated for its efficiency in extracting high-quality genomic DNA from leaf tissues of GF 677 and MaxMa-14 Prunus rootstocks. The integrity of the isolated DNA was initially assessed by agarose gel electrophoresis, which revealed intact, high-molecular-weight genomic DNA with no evidence of degradation. Spectrophotometric analyses showed that DNA concentrations ranged from 200–285 ng/µl for GF 677 and 260–300 ng/µl for MaxMa-14. The A260/280 and A260/230 absorbance ratios fell within the accepted ranges, indicating minimal contamination by proteins, polysaccharides, and phenolic compounds. The quality of the extracted DNA was further confirmed by successful restriction enzyme digestion using BamHI and HindIII, demonstrating the effective removal of inhibitory substances. In addition, PCR amplification using ISSR and SCoT marker systems produced clear, bright, and reproducible banding patterns, confirming the suitability of the isolated DNA for PCR-based molecular analyses.

Overall, the results demonstrate that the applied CTAB-based protocol provides a reliable, cost-effective, and practical approach for isolating high-quality genomic DNA from Prunus rootstocks. This method is suitable for routine laboratory use and supports downstream molecular applications such as marker-based genetic analyses and clonal identity verification.

Keywords

Supporting Institution

ESOGÜ BAP

Project Number

ESOGÜ BAP 202023A110

Thanks

This study utilizes data from the MSc. thesis titled “Determination of the Susceptibility of Some Pear Varieties and Genotypes to Fire Blight Disease Using Chemical Analysis,” conducted by Ahmet Kürşat Ölmez at the Eskişehir Osmangazi University, Faculty of Agriculture, Department of Horticulture. The study was supported by the Scientific Research Projects Commission (BAP) of Eskişehir Osmangazi University under project number 202023A101.

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Details

Primary Language

English

Subjects

Pomology and Treatment

Journal Section

Research Article

Authors

Mücahit Furkan Yeşilbaş
0000-0003-1484-9390
Türkiye

Emel Sözen
0000-0003-4859-8198
Türkiye

Yasemin Evrenosoğlu ^*
0000-0002-0212-8492
Türkiye

Publication Date

April 7, 2026

Submission Date

February 16, 2026

Acceptance Date

March 13, 2026

Published in Issue

Year 2026 Volume: 10 Number: 1

DOI

https://doi.org/10.66598/ijafls.1888824

IZ

https://izlik.org/JA56MT73EC

Cite

RIS / Bibtex

APA

Yeşilbaş, M. F., Sözen, E., & Evrenosoğlu, Y. (2026). Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers. International Journal of Agriculture Forestry and Life Sciences, 10(1). https://doi.org/10.66598/ijafls.1888824

AMA

1.Yeşilbaş MF, Sözen E, Evrenosoğlu Y. Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers. Int J Agric For Life Sci. 2026;10(1). doi:10.66598/ijafls.1888824

Chicago

Yeşilbaş, Mücahit Furkan, Emel Sözen, and Yasemin Evrenosoğlu. 2026. “Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers”. International Journal of Agriculture Forestry and Life Sciences 10 (1). https://doi.org/10.66598/ijafls.1888824.

EndNote

Yeşilbaş MF, Sözen E, Evrenosoğlu Y (April 1, 2026) Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers. International Journal of Agriculture Forestry and Life Sciences 10 1

IEEE

[1]M. F. Yeşilbaş, E. Sözen, and Y. Evrenosoğlu, “Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers”, Int J Agric For Life Sci, vol. 10, no. 1, Apr. 2026, doi: 10.66598/ijafls.1888824.

ISNAD

Yeşilbaş, Mücahit Furkan - Sözen, Emel - Evrenosoğlu, Yasemin. “Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers”. International Journal of Agriculture Forestry and Life Sciences 10/1 (April 1, 2026). https://doi.org/10.66598/ijafls.1888824.

JAMA

1.Yeşilbaş MF, Sözen E, Evrenosoğlu Y. Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers. Int J Agric For Life Sci. 2026;10. doi:10.66598/ijafls.1888824.

MLA

Yeşilbaş, Mücahit Furkan, et al. “Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers”. International Journal of Agriculture Forestry and Life Sciences, vol. 10, no. 1, Apr. 2026, doi:10.66598/ijafls.1888824.

Vancouver

1.Mücahit Furkan Yeşilbaş, Emel Sözen, Yasemin Evrenosoğlu. Efficient Genomic DNA Isolation and Quality Assessment in Prunus Rootstocks GF-677 and MaxMa-14 Using ISSR and SCoT Markers. Int J Agric For Life Sci. 2026 Apr. 1;10(1). doi:10.66598/ijafls.1888824