BIOCIDAL ACTIVITIES OF A TRITERPENOID SAPONIN AND FLAVONOID EXTRACTS FROM THE ERICA MANIPULIFLORA SALISB. AGAINST MICROFOULING BACTERIA

Abstract

Investigations on the development of environmentally friendly, non-toxic products in the antifouling paint technology are becoming more and more widespread. The main purpose of this study is to investigate biocidal activities of fractions belonging to n-BuOH which is one of the polar extracts derived from the plant Erica manipuliflora Salisb. against marine biofilm bacteria, and to carry out a basic research in order to use the extracts determined to have high activity as antifouling agents. Of the fractions belonging to the n-BuOH extract, EBA-EBB-EBC (flavonoids, phenylethanoid glycosides) and EBD-EBE (triterpenoid saponins) were tested against marine biofilm bacteria (Pseudoalteromonas, Alteromonas, Exiguobacterium, Vibrio species) at varying concentrations. Inhibition concentrations of the fractions against marine biofilm bacteria were analyzed with the MIC test and disk diffusion method. As a result of the screening of inhibition concentrations of the n-BuOH fractions obtained from Erica manipuliflora Salisb., the species Alteromonas and Exiguobacterium were determined as the most sensitive bacteria. The fractions EBA, EBD and EBB were more effective. At the end of this study, it was assessed that n-BuOH fractions rich in flavonoids and triterpenoid saponins exhibited activity against some marine biofilm bacteria. It was also determined that it would be worth doing further studies in which pure substances to be obtained could be used as an antifouling additive in industrial areas to prevent marine biofilms. 

Keywords

• Erica manipuliflora Salisb.,biocidal activity,marine biofilm bacteria,flavonoids,triterpenoid saponins

References

1. Adonizio A., Kong K.F., Mathee K., 2008. Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by South Florida plant extracts. Antimicrobial Agents and Chemotherapy, 52, 198–203.
2. Alma M.H., Mavi A., Yildirim A., Digrak M., Hirata T., 2003. Screening chemical composition and in vitro antioxidant and antimicrobial activities of the essential oils from Origanum syriacum L. growing in Turkey. Biochemical Pharmacology Bullettins, 26, 1725-1729.
3. Andrade S.F., Cardoso L.G.V., Carvalho J.C.T., Bastos J.K., 2007. Anti inflammatory and anti nociceptive activities of extract, fractions and populnoic acid from bark wood of Austroplenckia populnea. Journal of Ethnopharmacology, 109, 464-471.
4. Baytop T., 1999. Therapy with medicinal plants of Turkey. 2nd ed. The Nobel Publication: Istanbul, 208.
5. Bradshaw L.J., 1992. Laboratory Microbiology. 4th ed. Harcourt Brace Publishers: California, 435.
6. Chabot S., Bel-Rhlid R., Chenevert R., Piche, Y., 1992. Hyphal growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker and Hall, by the activity of structurally specific flavonoid compounds under CO2-enriched conditions. New Phytologist, 122, 461–467.
7. Chambers L.D., Stokes K.R., Walsh F.C., Wood, R.J.K., 2006. Modern approaches to marine antifouling coatings. Surface & Coatings Technology, 201, 3642–3652. Cowan, M.M., 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12, 564-582.
8. Cushnie, T.P.T., Lamb A., 2005. Antimicrobial activity of flavonoids. International Journal of Antimicrobial Agents, 26, 343–356.

9. Dulger G., Aki C., 2009. Antimicrobial Activity of the Leaves of Endemic Stachys pseudopinardii in Turkey. Tropical Journal of Pharmaceutical Research, 8, 371-375.
10. Garza, B.A.A., Gonzalez G.M.G., Aranda R.S., Torres N.W., Galindo V.M.R., 2007. Screening of antifungal activity of plants from the northeast of Mexico. Journal of Ethnopharmacology, 114,468-471.
11. Gershenzon J., Dudareva N., 2007. The function of terpene natural products in the natural world. Nature Chemical Biology, 3, 408– 414.
12. Hancock V., Dahl M., Vejborg R.M., Klemm P., 2010. Dietary plant components ellagic acid and tannic acid inhibit Escherichia coli biofilm formation. Journal of Medical Microbiology, 59, 496–498.
13. Inatani R., Nakatani N., Fuwa H., 1996. Antioxidative effect the constituent of rosemary (Rosmarinus officinalis L.) and their derivatives. Agricultural and Biological Chemistry, 47, 521-525.
14. Kacar A., Kocyigit A., Ozdemir G., Cihangir B., 2009. The Development of Biofilm Bacteria on Panels Coated by Different Antifouling Paints in the Marinas. Fresenius Environmental Bulletin, 18, 2004-2012.
15. Kang C.G., Hah D.S., Kim C.H., Kim Y.H., Kim E., Kim, J.S., 2011. Evaluation of Antimicrobial Activity of the Methanol Extracts from 8 Traditional Medicinal Plants. Toxicology Research, 27,31-36.
16. Kim J., Marshall M.R.A., Wei C., 1995. Antibacterial activity of some essential oil components against five foodborne pathogens. Journal of Agricultural and Food Chemistry, 43, 2839-2845.
17. Kotzekidou P., Giannakidis P., Boulamatsis A., 2008. Antimicrobial activity of some plant extracts and essential oils against food borne pathogens in vitro and on the fate of inoculated pathogens in chocolate. Journal of Food Science and Technology, 41, 119-127.
18. Kuete V., Nguemeving J.R., Beng V.P., Azebaze A.G.B., Etoa F.X., Meyer M., Bodo B., Nkengfack, A.E., 2007. Antimicrobial activity of the methanolic extracts and compounds from vismia laurentii De Wild (Guttiferae). Journal of Ethnopharmacology, 109, 372-379.
19. Lee J.H., Cho H.S., Joo S.W., Regmi S.C., Kim J.A., Ryu C.M., Ryu S.Y., Cho M.H., Lee, J., 2013. Diverse plant extracts and trans-resveratrol inhibit biofilm formation and swarming of Escherichia coli O157:H7. Biofouling, 29, 1189–1203.
20. Lee J.H., Cho M.H., Lee J., 2011a. 3-Indolylacetonitrile decreases Escherichia coli O157:H7 biofilm formation and Pseudomonas aeruginosa virulence. Environmental Microbiology, 13, 62–73.
21. Lee J.H., Regmi S.C., Kim J.A., Cho M.H., Yun H., Lee C.S., Lee, J. 2011b. Apple flavonoid phloretin inhibits Escherichia coli O157:H7 biofilm formation and ameliorates colon inflammation in rats. Infection and Immunity, 79, 4819–4827.
22. Li J.W.H., Vederas J.C., 2009. Drug discovery and natural products: end of an era or an endless frontier? Science, 325, 161–165.
23. Macros-Arias C., Eraso E., Madariaga L., Quindos G., 2011. In vitro activities of natural products against oral Candida isolates from denture wearers. BMC Complementary and Alternative Medicine, 11, 119.
24. Marcucci M.C., Ferreres F., Viguera C., Bankova V.S., Castro S.L., Dantas, A.P., 2001. Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology, 74, 105-12.
25. NCCLS (National Committee for Clinical Laboratory Standards), 1999. Performance standards for antimicrobial susceptibility testing. In Ninth Informational Supplement, Pennsylvania USA: M 100-S9.
26. Okolo C.O., Johnson P.B., Abdurahman E.M., Aguye I.A., Hussaini I.M., 1995. Analgesic effect of Irvingia gabonensis stem bark extract. Journal of Ethnopharmacology, 45, 125-129.
27. Onsare J.G., Arora, D.S., 2014. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. Journal of Applied Microbiology, 118, 313-325.
28. Palombo E.A., 2009. Traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases. Evidence-Based Complementary and Alternative Medicine, 2011, 1-15.
29. Pauli A., 2006. Anticandidal low molecular compounds from higher plants with special reference to compounds from essential oils. Medicinal Research Reviews, 26, 223–268.
30. Quave C.L., Plano L.R., Pantuso T., Bennett B.C., 2008. Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology, 118, 418–428.
31. Rabe T., Staden J.V., 1997. Antibacterial activity of South African plants used for medicinal purposes. Journal of Ethnopharmacology, 56, 81-87.
32. Railkin A.I., 2004. Marine biofouling colonization processes and defenses. CRC Pres Inc., New York.
33. Rajeshwar Y., Gupta M., Mazumder U.K., 2005. In vitro lipid peroxidation and antimicrobial activity of Mucuna pruriens seeds. Iranian Journal of Pharmacology & Therapeutics, 4, 32-35.
34. Rasmussen T.B., Bjarnsholt T., Skindersoe M.E., Hentzer M., Kristoffersen P., Köte M., Nielsen J., Eberl L., Givskov M., 2005. Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector. Journal of Bacteriology, 187, 1799–1814.
35. Rates S.M.K., 2001. Plants as source of drugs. Toxicon, 39, 603-613.
36. Raut J.S. Shinde R.B. Chauhan N.M., Karuppayil S.M., 2013. Terpenoids of plant origin inhibit morphogenesis, adhesion, and biofilm formation by Candida albicans. Biofouling, 29, 87–96.
37. Ren D., Zuo R., González- Barrios A.F., Bedzyk L.A., Eldridge G.R., Pasmore M.E., Wood T.K., 2005. Differential gene expression for investigation of Escherichia coli biofilm inhibition by plant extract ursolic acid. Applied Microbiology and Biotechnology, 71, 4022–4034.
38. Rezende G.P.S, Pimenta F.C., Costa, L.R.S., 2006. Antimicrobial activity of two Brazilian commercial propolis extracts. Brazilian Journal of Oral Sciences, 5, 967-970.
39. Rodrigues de Araujo A., Quelemes P.V., Gomes-Perfeito M.L., De Lima L.I., Coêlho-Sá M., Nunes P.H.M., Joanitti G.A., Eaton P., dos Santos Soares M.J., de Souza de Almeida Leite J.R. 2015. Antibacterial, antibiofilm and cytotoxic activities of Terminalia fagifolia Mart. extract and fractions. Annals of Clinical Microbiology and Antimicrobials, 14, 1-10.
40. Schultz M.P., Swain G.W., 2000. The influence of biofilms on skin friction drag. Biofouling, 15, 129-139.
41. Singh M., Singh N., Khare P.B., Rawat A.K.S., 2008. Antimicrobial activity of some important Adiantum species used traditionally in indigenous systems of medicine. Journal of Ethnopharmacology, 115, 327-329.
42. Stevens P.F., 1978. Flora of Turkey and the Aegean Islands. In: Davis, PH (Ed.), in Erica L. vol. 6. University Press Edinburgh, pp. 95–97.
43. Tuzlaci E., Eryasar-Aymaz F.P., 2001. Turkish folk medicinal plants, Part IV: Gonen (Balıkesir). Fitoterapia, 72, 323–343.
44. Wagner H., Bladt S., 2009. Plant Drug Analysis. Springer, Hiedelberg, 2nd Edition, 84.
45. Webster D., Taschereau P., Belland R.J., Sand C., Rennie R.P., 2008. Antifungal activity of medicinal plants extracts; preliminary screening studies. Journal of Ethnopharmacology, 115, 140-146.
46. Wojnicz D., Kucharska A.Z., Sokol-Letowska A., Kicia M., Tichaczek-Goska D., 2012. Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli. Urological Research, 40, 683–697.
47. Vikram A., Jayaprakasha G.K., Jesudhasan P.R., Pillai S.D.A., Patil B S., 2010a. Suppression of bacterial cell-cell signalling, biofilm formation and type III secretion system by citrus flavonoids. Journal of Applied Microbiology, 109,515–527.
48. Vikram A., Jesudhasan P.R., Jayaprakasha G.K., Pillai B.S., Patil B.S., 2010b. Grapefruit bioactive limonoids modulate E. coli O157:H7 TTSS and biofilm. International Journal of Food Microbiology, 140, 109–116.
49. Yebra D.M., Kiil S., Weinell C.E., Johansen K.D., 2006. Presence and effects of marine microbial biofilms on biocide-based antifouling paints. Biofouling, 22, 33-41.
50. Zgoda J.R., Porter J.R., 2001. A Convenient Microdilution Method for Screening Natural Products Against Bacteria and Fungi. Pharmaceutical Biology, 39, 221-225.
51. Zhao J., Davis L.C., Verpoorte R., 2005. Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnology Advances, 23,283–333.
52. Zore G.B., Thakre A.D., Jadhav S., Karuppayil S.M., 2011. Terpenoids inhibit Candida albicans growth by affecting membrane integrity and arrest of cell cycle. Phytomedicine, 18,1181–1190.