BIOCIDAL ACTIVITIES OF A TRITERPENOID SAPONIN AND FLAVONOID EXTRACTS FROM THE ERICA MANIPULIFLORA SALISB. AGAINST MICROFOULING BACTERIA

Year 2018, Volume: 2 Issue: 2, 40 - 46, 08.12.2018

Asli Kacar Sibel Avunduk Burcu Omuzbuken , Eray Aykın

Abstract

Investigations on
the development of environmentally friendly, non-toxic products in the
antifouling paint technology are becoming more and more widespread. The main
purpose of this study is to investigate biocidal activities of fractions
belonging to n-BuOH which is one of the polar extracts derived from the plant
Erica manipuliflora Salisb. against marine biofilm bacteria, and to carry out a
basic research in order to use the extracts determined to have high activity as
antifouling agents. Of the fractions belonging to the n-BuOH extract,
EBA-EBB-EBC (flavonoids, phenylethanoid glycosides) and EBD-EBE (triterpenoid
saponins) were tested against marine biofilm bacteria (Pseudoalteromonas,
Alteromonas, Exiguobacterium, Vibrio species) at varying concentrations.
Inhibition concentrations of the fractions against marine biofilm bacteria were
analyzed with the MIC test and disk diffusion method. As a result of the
screening of inhibition concentrations of the n-BuOH fractions obtained from
Erica manipuliflora Salisb., the species Alteromonas and Exiguobacterium were
determined as the most sensitive bacteria. The fractions EBA, EBD and EBB were
more effective. At the end of this study, it was assessed that n-BuOH fractions
rich in flavonoids and triterpenoid saponins exhibited activity against some
marine biofilm bacteria. It was also determined that it would be worth doing
further studies in which pure substances to be obtained could be used as an
antifouling additive in industrial areas to prevent marine biofilms. 

Keywords

Erica manipuliflora Salisb. , biocidal activity , marine biofilm bacteria , flavonoids , triterpenoid saponins

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