POLYGENIC RESISTANCE OF IMPROVED RED PEPPER LINES TO PHYTOPHTHORA CAPSICI

Abstract

Belonging to the Oomycete genus, Phytophthora capsici is responsible of many devastating disease and the most destructive agent of pepper in the World.  The most cost-effective method for preventing Phytophthora blight is to use resistant cultivars. In this study, the polygenic resistance of improved pepper lines to Phytophthora Blight were screened by molecular markers placed on three chromosomes in pepper. Sena variety registered as suitable for spice pepper production was used as susceptible female donor and CM 334 and Perennial genotypes were used as male resistance sources. Thirty two genotypes were screened by ASC 035 marker placed on chromosomes P10, ASC 037 on P5 and ASC 031 on P2. Twelve of these improved red pepper lines from different resistance sources were exhibited polygenic resistance according to electrophoretic signals to all of three markers placed on P2, P5 and P10. In order to determine codominance of the resistance genes PCR products of ASC 031 and ASC 037 were restricted by HaeIII and EcoRI respectively. Ten improved lines exhibited codominance related with resistance to Phytophthora Blight concerning ASC 031 and only one line was showed codominance for ASC 037. Three different lines had one resistance gene on P5 and on P10 independently. Only T12 line holds two resistance genes both P2 and P10. DD and 63 lines were most promising genotypes related with their resistance to pathogen. Molecular assisted selection was found useful for improving Phytophthora resistant red pepper lines. 

Keywords

• Phytophthora capsici,polygenic resistance,molecular markers,breeding

References

1. Abak, K. 1982. Biberlerde Kökboğazı Yanıklığına dayanıklılığın kalıtımı üzerinde araştırmalar. A.Ü.Ziraat Fakültesi, Bakçe Bitkileri Bölümü, Doçentlik Tezi, Ankara.
2. Choi, K. S., Young, H. O., Cang, H. L. and Jae, W. L. 1985. Studies On Varietal Differences and Inheritance of Resistance to Phytophtora capsici In Red Peppers Of Korea. Capsicum and Eggplant Newsletter, 5:39-40 p.
3. Galmarini, C. R. 1997. Pepper Breeding in Argentina. Capsicum and Eggplant Newsletter. 16: 28-34.
4. Guerrero-Moreno, A. and Laborde, J.A. 1980. Current Status of Pepper Breeding for Resistance to Phytophthora capsici in Mexico. IV. Meeting Eucarpian Capsicum Working Group. Wageningen: 52-56.
5. İren, S., Maden, S., Patlıcangil, B., & Etmeni, K. T. B. Y. H. Phytopthora capsici Leon Enfeksiyonlarına Karşı Serada Reaksiyonlarının Tespiti. Ankara Üniversitesi Ziraat Fakültesi Yıllığı, 26, 323-330.
6. Kim, E. S. and Hwang, B. K. 1992. Virulence to Korean pepper cultivars of isolates of Phytophthora capsici from different geographic aeras. Plant Disease 76:486-489.
7. Kiran, O. F., & Ertunc, F. 1998. Detection of the Disease of Solanaceous Plants in Van Province. JOURNAL OF TURKISH PHYTOPATHOLOGY, 27, 105-112.
8. Lee, B. K., Kim, B. S., Chang, S. W. and Hwang, B. K. 2001. Aggressiveness to pumpkin cultivars of isolates of Phytophthora capsici from pumpkin and pepper. Plant Dis. 85:497-500.

9. Lefebvre, V. and Palloix, A. 1996. Both additive and Epistatic Effects of QTLs are Involved in Polygenic Induced Resistance to Diseases: A Case Study, the Interaction Pepper- Phytophthora capsici Leon. Theor. Appl. Genet. 93: 503-511.
10. Leonian, LH. 1922. Stem and fruit blight of pepper caused by Phytophthora capsici sp. Nov. Phytopathology 12: 401-408.
11. Metsalu, T., & Vilo, J. (2015). ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap. Nucleic acids research, 43(W1), W566-W570.
12. Milkova, L., Vitanov, M. and Daskalov, S. 1988. Phytostop – A New Cultivar Resistant To Phytophtora capsici Capsicum and Eggplant Newsletter, 7: 62.
13. Pochard E.1964.Piment. Rapport, d’Activite 1964.Stat. d’Amelior.Plantes Maraich. d’Avignon (France):41-46.
14. Pochard, E. and Daubèze, A. M. 1982. Comparison of Three Different Sources of Resistance to Phytophthora capsici in Capsicum annuum. Capsicum and Eggplant Newsletter, 1: 59-62.
15. Ruffel, S., Gallois, J. L., Moury, B., Robaglia, C., Palloix, A., & Caranta, C. (2006). Simultaneous mutations in translation initiation factors eIF4E and eIF (iso) 4E are required to prevent pepper veinal mottle virus infection of pepper. Journal of General Virology, 87(7), 2089-2098.
16. Simon, J.J., Webster, R.K. 1979. Idendification of strains and inheritance of pathogenicity in Phytophthora capsici. Phytopathology, 62:20-26.
17. Smith, P. G., Kimble, K. A., Grogan, R. G. and Millett, A. H. 1967. Inheritance of resistance in peppers to Phytophthora root rot . Phytopathology. 57:377-379.
18. Sotirova, V. and Daskalov, S. 1983. Use of Induced Mutations In Developing Pepper Forms Resistant to Phytophtora capsici Leonian. Capsicum and Eggplant Newsletter, 2: 48-50
19. Soylu, S., & Kurt, Ş. 2001. Occurrence and distribution of fungal diseases on greenhouse grown pepper plants in Hatay Province. In International XIth EUCARPIA Meeting on Genetics and Breeding of Capsicum & Eggplant (pp. 315-319).
20. Thabuis, A., Lefebvre, V., Bernard, G., Daubeze, A. M., Phaly, T., Pochard, E., & Palloix, A. 2004. Phenotypic and molecular evaluation of a recurrent selection program for a polygenic resistance to Phytophthora capsici in pepper. Theoretical and Applied Genetics, 109(2), 342-351.
21. Thabuis, A., Palloix, A., Pflieger, S., Daubeze, A.M., Caranta, C. and Lefebvre, V. 2003. Comparative mapping of Phytophthora resistance loci in pepper germplasm: evidence for conserved resistance loci across Solanaceae and for a large genetic diversity. Theor. Appl. Genet. 106: 1473-1485.
22. Thabuis, A., Palloix, A., Servin, B., Daubeze, A. M., Signoret, P., & Lefebvre, V. 2004 a. Marker-assisted introgression of 4 Phytophthora capsici resistance QTL alleles into a bell pepper line: validation of additive and epistatic effects. Molecular Breeding, 14(1), 9-20.
23. Yamakawa K., Mochizuki T., Yasui H. 1979. Screening of Cultivated and Wildpeppers for Phytophthora capsici Resistance and Its Inheritance. Bull. Veg. And Ornam. Crops. Res.Stat. Japan Ser., A. (6): 29-38.