Effect of different plant growth regulator on in vitro propagation of endangered plant; yellow tomato (Lycopersicon esculentum Mill.)

Abstract

In the present study, a convinient procedure for in vitro propagation of yellow tomato plant, (Lycopersicon esculentum Mill.) under the risk of extinction, was developed. Shoot tip, hypocotyl, cotyledon, leaf, node and internode were excised from sprout seedlings and used for explant sources. In vitro adventitious shoot regeneration was achieved in MS medium supported with the particular concentrations and combinations of plant growth regulators (PGRs) via direct organogenesis. The most successful (100%) adventitious shoot regeneration was provided from node and shoot tip explants in MS medium supported with the particular concentrations and combinationss of 6-Benzylaminopruine (BAP) and Naphtalane acetic acid (NAA). The highest root formation (100%) was achieved on regenerated adventitious shoot in MS medium supported with Indole-3-acetic acid (IAA) and Indole-3-butyric acid (IBA). The whole seedlings regenerated in vitro were adapted to soil and acclimatized in field.

Keywords

• yellow tomato,micropropagation,plant growth regulators

References

1. BBS. (2007). Year book of agricultural statistics of bangladesh, bangladesh bureau of statistics, ministry of planning Govt. of the Peoples. Republic of Bangladesh Dhaka, 17.
2. Branca, C., G. Bucci, P. Domiano, A. Ricci, A. Torelli and M. Bassi (1990). Auxin structure and activity on tomato morphogenesis in vitro and pea stem elongation. Plant Cell Tissue Organ Culture. 24: 105-114.
3. Chirom, O., T. Doni, L. Tikendra and P. Nongdam (2015). Establishment of efficient in vitro culture and plantlet generation of tomato (Lycopersicon esculentum Mill.) and development of synthetic seeds. Journal of Plant Sciences. 10 (1): 15-24.
4. Compton, M.E. and R.E. Veillux (1991). Shoot root and flower morphogenesis on tomato inflorescence explants. Plant Cell Tissue Organ Culture. 24: 223–231.
5. Durrani, N.S., D. Ahmad, A. Jalal, H. Rajab and M.S. Khan (2017). The effect of explant sources and growth regulators on callus induction and regeneration in different tomato cultivars. The Journal of Animal & Plant Sciences. 27(2): 481-489.
6. Feito, I., M.A. Gea, B. Ferna´ndez and R. Rodr´ıguez (1996). Endogenous plant growth regulators and rooting capacity of different walnut tissues. Plant Growth Regulation. 19: 101–108.
7. George, E.F., M.A. Hall and G.J. De-Klerk (2008). Plant propagation by tissue culture 3rd Edition, Springer, Dordrecht, The Netherlands 1: 501.
8. Gisbert, C., J. Prothens and F. Nuez (2006). Efficient regeneration in two potential new crops for subtropical climates, the scarlet (Solanum aethiopicum) and gboma (S. macrocarpon) eggplants. New Zealand Journal of Crop and Horticulturel Science. 34: 55-62.

9. Gubis, J., Z. Lajchova, J. Farago and Z. Jurekova (2004). Effect of growth regulators on shoot induction and plant regeneration in tomato (Lycopersicon esculentum Mill.). Biologia Bratislava. 59(3): 405-408.
10. Hanus-Fajerska, E. (2006). Variation in tomato plants regenerated from cucumber mosaic viruse infected tissue. ISHS Acta Hort789: XV Meeting of the EUCARPIA Tomato Working Group. http:// www.actahort.org/books/789/789_40.htm
11. Hatzilazarou, S.P., T.D. Syros, T.A. Yupsanis, A.M. Bosabalidis and A.S. Econo- mou (2006). Peroxidases, ligin and anatomy during in vitro and ex vitro rooting of gardenia (Gardenia jasminoides Ellis) microshoots. Journal of Plant Physiology. 163: 827–836
12. Henry, Y., P. Vain and J.D. Buyser (1994). Genetic analysis of in vitro plant tissue culture responses and regeneration capacities. Euphytica. 79: 45-58.
13. Jamous, F. and Abu-Qaoud H. (2015). In vitro regeneration of tomato (Lycopersicon esculentum Mill). Plant Cell Biotechnology and Molecular Biology. 16(3&4):181-190.
14. Kanna, S.V. and N. Jayabalan (2010). Influence of n6-(2-isopentenyl) adenine on in vitro shoot proliferation in Solanum melongena. International Journal of Academic Research 2(2): 98.
15. Kaur, M., A.S. Dhatt, J.S. Sandhu and A.S. Sidhu (2011). Role of genotype, explant and growth hormones on regeneration in eggplant (Solanum melongena L.). Indian Journal of Agricultural Science. 81(1): 38-43.
16. Kumar, N. and M.P. Reddy (2010). Plant regeneration through the direct induction of shoot buds from petiole explants of Jatropha curcas: a biofuel plant. Annals of Applied Biology. 156: 367-375.
17. Kumar, N., K.G. Vijayanand and M.P. Reddy (2011). Plant regeneration in non-toxic Jatropha curcas - impacts of plant growth regulators, source and type of explants. Journal of Plant Biochemistry and Biotechnology. 20: 125-133.
18. Li, T., J.K. Sun, Z.H. Lu and Q. Liu (2011). Transformation of HBsAg (Hepatitis B Surface Antigen) gene into tomato mediated by Agrobacterium tumefaciens. Czech Journal of Genetics and Plant Breeding. 2: 69–77.
19. Lindsey, K. (1992). Genetic manipulation of crop plants. Journal of Biotechnology. 26: 1-28.
20. Liza Lutfun Nahar, A.N.M., K.M.A. Nasar, M. Zinnah, C. Al Nayem and M. Ashrafuzzaman (2013). In Vitro growth media effect for regeneration of tomato (Lycopersicon esculentum) and evaluationof the salt tolerance activity of callus. Journal of Agriculture and Sustainability. 3(2): 132-143.
21. Locy, R.D. (1981). Callus formation and organogenesis by explants of six Lycopersicon spp. Canadian Journal of Botany. 61: 1072- 1079.
22. McCormic, S., J. Niedermeyer, B. Fry, A. Barnason, R. Horch and R. Farley (1986). Leaf disk transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens. Plant Cell Reports. 5: 81-84.
23. McGlasson, B. (2003). Tomatoes. Encyclopedia of food sciences and nutrition (second edition), 5800-5808.
24. Moghaieb, R.E.A., H. Saneoka and K. Fujita (1999). Plant regeneration from hypocotyls and cotyledon explants of tomato (Lycopersicon esculentum). Soil Science and Plant Nutrition. 45: 639-646.
25. Moncalean, P., A. Rodriguez and B. Fernandez (2001). In vitro response of Actinidia deliciosa explants to different BA incubation periods. Plant Cell Tissue Organ Culture. 67: 257–266.
26. Motte, H., D. Vereecke, D. Geelen and S. Werbrouk (2013). The molecular path to in vitro shoot regeneration. Biotechnology Advances. 32(1): 107-121.
27. Murashige, T. and F. Skoog (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Journal of Plant Physiology. 15: 473- 497.
28. Namitha, K.K. and P. Negi (2013). Morphogenetic potential of tomato (Lycopersicon esculentum,) cv. Arka Ahuti to plant growth regulators. Notulae Scientia Biologicae. 2: 220–225.
29. Oktem, H.A., M. Mahmoudian, F. Eyido and M. Yücel (1999). Gus gene delivery and expression in lentil cotyledonary nodes using particle bombardment. Lens Newslett. 26: 3-6.
30. Ouyang, B., X. Li-Han and B. Ye-Zhi (2003). Effects of zeatin and IAA on plant regeneration of tomato cotyledon explants. Plant Physiology Communications. 39: 217-218.
31. Park, J.L., J.E. Morris, K.D. Park and R.H. Hirschi (2003). Efficient and genotype independent Agrobacterium - mediated tomato transformation. Journal of Plant Physiology. 160(10): 1253-1257.
32. Plana, D., A. Marta, M.L. Regla, F. Marilyn, F. Alvarez and C. Moya (2005). A new in vitro regeneration protocol in tomato (Lycopersicon esculentum). Cultivos Tropicales. 26(2): 17-20.
33. Preil, W. (2003). Micropropagation of ornamental plants. In: Laimer M, Rucker W, editors. Plant tissue culture 100 years since Gottlieb Haberlandt. New York: Springer-Verlag. 115-133.
34. Rout, G.R. and S.M. Jain (2004). Micropropagation of ornamental plants-cut flowers. Propagation of Ornamental Plants. 4: 3-28.
35. Schween, G. and H.G. Schwenkel (2003). Effect of genotype on callus induction, shoot regeneration, and phenotypic stability of regenerated plants in greenhouse of Primula ssp. Plant Cell Tissue Organ Culture. 72: 53-61.
36. Slater, A., N. Scott and M. Fowler (2003). Plant Biotechnology: The Genetic Manipulation of Plants. Oxford University Press Inc, New York. 42.
37. Su, Y.H. and X.S. Zhang (2014). The hormonal control of regeneration in plants. Current Topics in Developmental Biology. 108: 35-69.
38. Taiz, L. and E. Zeiger (1991). Plant Physiology. Redwood City: The Benjamin/Cumings Publishing.
39. Takashina, T., T. Suzuki, H. Engashira and S. Imanishi (1998). New molecular markers linked with high shoot regeneration capacity of wild tomato species (Lycopersicon chilense). Breeding Science. 48(2): 109-113.
40. Teng, W.L. (1999). Source, etiolation and orientation of explants affect in vitro regeneration of Venus fly-trap (Dionaea muscipula). Plant Cell Reports. 18: 363-368.
41. Valero-Aracama, C., M. Kane, S. Wilson and N. Philman (2010). Substitution of benzyladenine with metatopolin during shoot multiplication increases acclimatization of difficult and easy to acclimatize sea oats (Uniola paniculata L.) genotypes. Plant Growth Regulation. 60: 43–49.
42. VanRoeke, J.S.C., B. Damm, L.S. Melchers and A. Hoekema (1993). Factors influencing transformation frequency of tomato. Plant Cell Reports. 12: 644-647.
43. Vural, H., E. Iyok and D. Duman (2000). Kültür sebzeleri. Ege üniversitesi basımevi. İzmir.
44. Young, R., V. Kaul and E.G. Williams (1987). Clonal propagation in vitro from immature embryos and flower buds of Lycopersicon peruvianum and L. esculentum. Plant Science. 52: 237–242.