Effect of culture filtrate concentration of Rhizoctonia solani Kühn against Meloidogyne incognita and Meloidogyne hapla in vitro

Abstract

The effect of culture filtrates of concentration of Rhizoctonia solani Kühn against Meloidogyne incognita and Meloidogyne hapla on juvenile mortality, hatching of egg masses and individual eggs in vitro has been investigated. The Rhizoctonia solani culture filtrate was diluted from 100% to make 75%, 50% and 25% concentrations. While hatched juvenile of M. incognita and M. hapla were counted after 7 days and juvenile mortality of M. incognita and M. hapla counted after 24 h. Culture filtrates of R.solani showed negative effects on M. incognita and M. hapla eggs and juveniles and directly proportional to the concentration of culture filtrates. The highest negative effect was found on juveniles in both nematode species. The negative effects on egg hatching and juvenile mortality of R. solani on M. hapla was found to be lower than M. incognita. This results showed that R. solani culture filtrates showed toxic effects on M. incognita and M. hapla eggs and juveniles in vitro and nematode species was important in this effect.

Keywords

• Rhizoctonia solani
• root knot nematode
• egg- hatching
• juvenile mortality
• antagonism

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