Year 2021, Volume 5 , Issue 1, Pages 106 - 112 2021-06-30

Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber

Ferdinand ARCALA [1]


This study aimed to: (1) isolate entomopathogenic fungi from infected host insects; (2) conduct bioassay of different entomopathogenic fungi (EPF) isolates against the cucurbit leafworm, Diaphania indica.; and (3) identify the most potential EPF isolate using morphological and molecular characterization. Four entomopathogenic fungi isolates that showed positive infection to D. indica larvae were evaluated. The most virulent isolate was subjected to morphological and molecular characterization. Results showed that EPF Isolate 1 is highly virulent to D. indica larvae with 97.5-100% mortality in the 2nd to 4th instar larvae. EPF Isolate 1 was first to show infection 1.38±0.41 days after inoculation, and larvae were entirely covered with white mycelia 5.25±0.41 days. Molecular identification revealed the isolate as Isaria fumosorosea as indicated by molecular marker internal transcribed space region of 5.8s (ITS-5.8s) ribosomal DNA. EPF Isolate 1 was identified as Isaria fomosorosea. It is the most virulent and potential isolate against cucurbit leafworm, D. indica.
Entomopathogenic fungi (EPF), Isaria fumosorosea, Diaphania indica, bioassay
  • Yazdi, F. K., Ezlenberg, J. and Mohammadipour, A. 2011. Biological Characterization of Beauveria bassiana (Clavicipitaceae: Hypocreales) from Overwintering Sites of Sunn Pest, Eurygaster integriceps (Scutelleridae: Heteroptera) in Iran. International Journal of Agricultural Science and Research, 2(2): 1-12.
  • Zimmermann, G. 2008. The entomopathogenic fungi Isaria farinose (formerly Paecilomyces farinosus) and the Isaria fumosorosea species complex (formerly Paecilomyces fumosoroseus): biology, ecology and use in biological control. Biocontrol Science and Technology 18 (9): 865-901.
Primary Language en
Subjects Plant Science
Journal Section Original Papers
Authors

Orcid: 0000-0001-7347-1506
Author: Ferdinand ARCALA (Primary Author)
Institution: Nueva Vizcaya State University
Country: Philippines


Supporting Institution Nueva Vizcaya State University
Project Number none
Thanks Thanks to College of Agriculture, Nueva Vizcaya State University.
Dates

Application Date : December 17, 2020
Acceptance Date : June 8, 2021
Publication Date : June 30, 2021

Bibtex @research article { ijafls841779, journal = {International Journal of Agriculture Forestry and Life Sciences}, issn = {}, eissn = {2602-4381}, address = {}, publisher = {Volkan OKATAN}, year = {2021}, volume = {5}, pages = {106 - 112}, doi = {}, title = {Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber}, key = {cite}, author = {Arcala, Ferdinand} }
APA Arcala, F . (2021). Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber . International Journal of Agriculture Forestry and Life Sciences , 5 (1) , 106-112 . Retrieved from https://dergipark.org.tr/en/pub/ijafls/issue/60445/841779
MLA Arcala, F . "Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber" . International Journal of Agriculture Forestry and Life Sciences 5 (2021 ): 106-112 <https://dergipark.org.tr/en/pub/ijafls/issue/60445/841779>
Chicago Arcala, F . "Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber". International Journal of Agriculture Forestry and Life Sciences 5 (2021 ): 106-112
RIS TY - JOUR T1 - Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber AU - Ferdinand Arcala Y1 - 2021 PY - 2021 N1 - DO - T2 - International Journal of Agriculture Forestry and Life Sciences JF - Journal JO - JOR SP - 106 EP - 112 VL - 5 IS - 1 SN - -2602-4381 M3 - UR - Y2 - 2021 ER -
EndNote %0 International Journal of Agriculture Forestry and Life Sciences Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber %A Ferdinand Arcala %T Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber %D 2021 %J International Journal of Agriculture Forestry and Life Sciences %P -2602-4381 %V 5 %N 1 %R %U
ISNAD Arcala, Ferdinand . "Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber". International Journal of Agriculture Forestry and Life Sciences 5 / 1 (June 2021): 106-112 .
AMA Arcala F . Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. Int J Agric For Life Sci. 2021; 5(1): 106-112.
Vancouver Arcala F . Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber. International Journal of Agriculture Forestry and Life Sciences. 2021; 5(1): 106-112.
IEEE F. Arcala , "Morphological and molecular characterization of fungal entomopathogen Isaria fumosorosea (Wize) Brown and Smith as bioinsecticide against cucurbit leafworm, Diaphania indica Saunders, in cucumber", International Journal of Agriculture Forestry and Life Sciences, vol. 5, no. 1, pp. 106-112, Jun. 2021