Research Article
BibTex RIS Cite

Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei

Year 2021, Volume: 5 Issue: 1, 87 - 93, 30.06.2021

Abstract

With a cosmopolite widespread worldwide, Ascocyhta rabiei may result in yield losses in chickpea fields up to 100% when the climate conditions were available for growth and development of the pathogen. Effects of culture media, temperature, pH and light regimes on mycelial growth of nine A. rabiei isolates isolated from different agroecological zones of Yozgat province (Turkey) were investigated in this study. It was observed that culture media, temperature, pH and light regimes significantly influenced mycelial growth rates of A. rabiei isolates. A. rabiei isolated developed smooth, sparse and immersed mycelium in culture media. Mycelium was characterized with pale cream, light greenish grey or grey-black color. Malt Extract Agar (MEA) medium was identified as the best culture medium promoting mycelial growth; the temperature required for maximum daily mycelial growth (2.0 mm day-1) was identified as 20.0°C. Present isolates formed smaller-diameter colonies at acidic pH values as compared to basic pH values. The pH values of 6.0 and 7.0 promoted mycelial growth. Light significantly influenced radial mycelial growth of YBUAr7 isolate of A. rabiei. Prolonged light exposures promoted radial growth in culture media and the cultures incubated under continuous light exhibited greater radial growth rates than the cultures incubated under 12 h photoperiods and 24 h dark.

Supporting Institution

Yozgat Bozok University Scientific Research Project Coordination Unit

Project Number

6602c-ZF/18-231

Thanks

The author is grateful for the financial support provided for this research by Yozgat Bozok University Scientific Research Project Coordination Unit (Project no: 6602c-ZF/18-231)

References

  • Abubakar, A. Suberu, H.A. Bello, I.M. Abdulkadir, R. Daudu, O.A. Lateef, A. A. (2013). Effect of pH on mycelial growth and sporulation of Aspergillus parasiticus. J. Plant Sci., 1. 4: 64-67
  • Attar, B. Ahmed, S. Kayim, M. Choueiri, E. Ghannam, H.A. Hamwieh, A. (2020). Role of sexual reproduction in the aggressivene.s of Didymella rabiei affecting chickpea. Arab. J. Plant Prot., 38.1: 17-24 https://dx.doi.org/10.22268/AJPP-38.1.010016
  • Aveskamp, M.M. de Gruyter, J. Woudenberg, J.H.C. Verkley, G.J.M. Crous, P.W. (2010). Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related Pleosporalean genera. Stud. Mycol., 65: 1-60 https://doi.org/10.3114/sim.2010.65.01
  • Bahr, L. María Victoria Castelli, M.V. Barolo, M.I. Ruiz Mostacero, N. Tosello, M.E. López, S.N. (2016). Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei. Fungal Biol., 120.3: 424-432 http://dx.doi.org/10.1016/j.funbio.2015.12.002
  • Baite, M.S. Dubey, S.C. Singh, B. (2016). Morphological variability in the Indian isolates of Ascochyta rabiei causing blight in chickpea and evaluation of chickpea cultivars. Indian J. Plant Prot., 44.1: 74-82
  • Basandri, A.K. Pande, S. Kishore, G.K. Crouch, J.H. Basandri, D. (2005). Cultural, morphological and pathological variation in Indian isolates of Ascochyta rabiei, the chickpea blight pathogen. Plant Pathol. J., 21.3:207-213 https://doi.org/10.5423/PPJ.2005.21.3.207
  • Bayraktar, H. Ozer, G. Aydogan, A. Palacıoglu, G. (2016). Determination of Ascochyta blight disease in chickpea using Real-Time PCR. J. Plant Dis. Prot., 123.3: 109-117 https://doi.org/10.1007/s41348-016-0017-0
  • Benzohra, I.E. Bendahmane, S.B. Benkada, M.Y. (2017). Effect of five temperature levels on mycelial growth and sporulation of Botrytis fabae, agent of chocolate spot on faba bean (Vicia faba L. Subsp. Major). American-Eurasian J. Agric. & Environ. Sci., 17.6: 530-535 DOI: 10.5829/idosi.aejaes.2017.530.535
  • Deshmukh, A.J. Mehta, B.P. Sabalpara, A.N. Patil, V.A. (2012). In vitro effect of various nitrogen, carbon sources and pH regimes on the growth and sporulation of Colletotrichum gloeosporioides Penz. and Sacc causing anthracnose of Indian bean. J. Biopest, 5 (Supplementary): 46-49
  • Duzdemir, Oral. Selvi, B. Yanar, Y. Yildirimi, A. (2014). Sources of resistance in chickpea (Cicer arietinum L.) land races against Ascochyta rabiei causal agent of Ascochyta blight disease. Pak. J. Bot., 46.4: 1479-1483
  • Faostat 2019. The State of Food and Agriculture. Food and Agriculture Organization of the United Nations (FAO) http://www.fao.org/faostat/en/#data/QC (Accessed Date: 03.02.2021)
  • Harveson, R.M. Markell, S.G. Goswami, R. Urrea, C.A. Burrows, M.E. Dugan, F. Chen, W. Skoglund, L.G. (2011).Ascochyta blight of chickpeas. Plant Health Progress, 12:1 https://doi.org/10.1094/PHP-2011-0103-01-DG
  • Kaiser, W.J. (1973). Factors affecting growth, sporulation, pathogenicity, and survival of Ascochyta rabiei. Mycologia, 65.2: 444-457 https://doi.org/10.2307/3758115
  • Kosiada, T. (2012). In vitro growth of some species of Ascochyta Lib. Cent. Eur. J. Biol., 7.6: 1076-1083 https://doi.org/10.2478/s11535-012-0095-3
  • Ozkilinc, H. , Frenkel, O. Abbob, S. Eshed, R. Sherman, A. Shtienberg, D. Ophirc, R. C. Can, C. (2010). A comparative study of Turkish and Israeli populations of Didymella rabiei, the Ascochyta blight pathogen of chickpea. Plant Pathol., 59: 492–503 https://doi.org/10.1111/j.1365-3059.2009.02226.x
  • Pande, S. Siddique, K.H. Kishore, G.K. Bayaa, B. Gaur, P.M. Gowda, C.L. Bretag, T.W. Crouch, J.H. (2005). Ascochyta blight of chickpea (Cicer arietinum L.): A review of biology, pathogenicity, and disease management. Aust. J. Agric. Res., 56.4: 317-332 https://doi.org/10.1071/AR04143
  • Saha, A. Mandal, P. Dasgupta, S. Saha, D. (2008). Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl. J. Environ. Biol., 29: 407-10 https://www.researchgate.net/publication/23440306
  • Sharma, G. Pandey, R.R. (2010). Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes. J. Yeast Fungal Res.,1.8: 157-164 https://doi.org/10.5897/JYFR.9000029
  • Trapero-Casas, A. Luque-Márquez, F. Kaiser, W.J. (2012). Development of the teleomorph of Ascochyta rabiei on culture media. Eur. J. Plant Pathol., 134: 773–782 https://doi.org/10.1007/s10658-012-0053-y
Year 2021, Volume: 5 Issue: 1, 87 - 93, 30.06.2021

Abstract

Project Number

6602c-ZF/18-231

References

  • Abubakar, A. Suberu, H.A. Bello, I.M. Abdulkadir, R. Daudu, O.A. Lateef, A. A. (2013). Effect of pH on mycelial growth and sporulation of Aspergillus parasiticus. J. Plant Sci., 1. 4: 64-67
  • Attar, B. Ahmed, S. Kayim, M. Choueiri, E. Ghannam, H.A. Hamwieh, A. (2020). Role of sexual reproduction in the aggressivene.s of Didymella rabiei affecting chickpea. Arab. J. Plant Prot., 38.1: 17-24 https://dx.doi.org/10.22268/AJPP-38.1.010016
  • Aveskamp, M.M. de Gruyter, J. Woudenberg, J.H.C. Verkley, G.J.M. Crous, P.W. (2010). Highlights of the Didymellaceae: a polyphasic approach to characterise Phoma and related Pleosporalean genera. Stud. Mycol., 65: 1-60 https://doi.org/10.3114/sim.2010.65.01
  • Bahr, L. María Victoria Castelli, M.V. Barolo, M.I. Ruiz Mostacero, N. Tosello, M.E. López, S.N. (2016). Ascochyta blight: isolation, characterization, and development of a rapid method to detect inhibitors of the chickpea fungal pathogen Ascochyta rabiei. Fungal Biol., 120.3: 424-432 http://dx.doi.org/10.1016/j.funbio.2015.12.002
  • Baite, M.S. Dubey, S.C. Singh, B. (2016). Morphological variability in the Indian isolates of Ascochyta rabiei causing blight in chickpea and evaluation of chickpea cultivars. Indian J. Plant Prot., 44.1: 74-82
  • Basandri, A.K. Pande, S. Kishore, G.K. Crouch, J.H. Basandri, D. (2005). Cultural, morphological and pathological variation in Indian isolates of Ascochyta rabiei, the chickpea blight pathogen. Plant Pathol. J., 21.3:207-213 https://doi.org/10.5423/PPJ.2005.21.3.207
  • Bayraktar, H. Ozer, G. Aydogan, A. Palacıoglu, G. (2016). Determination of Ascochyta blight disease in chickpea using Real-Time PCR. J. Plant Dis. Prot., 123.3: 109-117 https://doi.org/10.1007/s41348-016-0017-0
  • Benzohra, I.E. Bendahmane, S.B. Benkada, M.Y. (2017). Effect of five temperature levels on mycelial growth and sporulation of Botrytis fabae, agent of chocolate spot on faba bean (Vicia faba L. Subsp. Major). American-Eurasian J. Agric. & Environ. Sci., 17.6: 530-535 DOI: 10.5829/idosi.aejaes.2017.530.535
  • Deshmukh, A.J. Mehta, B.P. Sabalpara, A.N. Patil, V.A. (2012). In vitro effect of various nitrogen, carbon sources and pH regimes on the growth and sporulation of Colletotrichum gloeosporioides Penz. and Sacc causing anthracnose of Indian bean. J. Biopest, 5 (Supplementary): 46-49
  • Duzdemir, Oral. Selvi, B. Yanar, Y. Yildirimi, A. (2014). Sources of resistance in chickpea (Cicer arietinum L.) land races against Ascochyta rabiei causal agent of Ascochyta blight disease. Pak. J. Bot., 46.4: 1479-1483
  • Faostat 2019. The State of Food and Agriculture. Food and Agriculture Organization of the United Nations (FAO) http://www.fao.org/faostat/en/#data/QC (Accessed Date: 03.02.2021)
  • Harveson, R.M. Markell, S.G. Goswami, R. Urrea, C.A. Burrows, M.E. Dugan, F. Chen, W. Skoglund, L.G. (2011).Ascochyta blight of chickpeas. Plant Health Progress, 12:1 https://doi.org/10.1094/PHP-2011-0103-01-DG
  • Kaiser, W.J. (1973). Factors affecting growth, sporulation, pathogenicity, and survival of Ascochyta rabiei. Mycologia, 65.2: 444-457 https://doi.org/10.2307/3758115
  • Kosiada, T. (2012). In vitro growth of some species of Ascochyta Lib. Cent. Eur. J. Biol., 7.6: 1076-1083 https://doi.org/10.2478/s11535-012-0095-3
  • Ozkilinc, H. , Frenkel, O. Abbob, S. Eshed, R. Sherman, A. Shtienberg, D. Ophirc, R. C. Can, C. (2010). A comparative study of Turkish and Israeli populations of Didymella rabiei, the Ascochyta blight pathogen of chickpea. Plant Pathol., 59: 492–503 https://doi.org/10.1111/j.1365-3059.2009.02226.x
  • Pande, S. Siddique, K.H. Kishore, G.K. Bayaa, B. Gaur, P.M. Gowda, C.L. Bretag, T.W. Crouch, J.H. (2005). Ascochyta blight of chickpea (Cicer arietinum L.): A review of biology, pathogenicity, and disease management. Aust. J. Agric. Res., 56.4: 317-332 https://doi.org/10.1071/AR04143
  • Saha, A. Mandal, P. Dasgupta, S. Saha, D. (2008). Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl. J. Environ. Biol., 29: 407-10 https://www.researchgate.net/publication/23440306
  • Sharma, G. Pandey, R.R. (2010). Influence of culture media on growth, colony character and sporulation of fungi isolated from decaying vegetable wastes. J. Yeast Fungal Res.,1.8: 157-164 https://doi.org/10.5897/JYFR.9000029
  • Trapero-Casas, A. Luque-Márquez, F. Kaiser, W.J. (2012). Development of the teleomorph of Ascochyta rabiei on culture media. Eur. J. Plant Pathol., 134: 773–782 https://doi.org/10.1007/s10658-012-0053-y
There are 19 citations in total.

Details

Primary Language English
Subjects Agricultural, Veterinary and Food Sciences
Journal Section Original Papers
Authors

Ali Endes 0000-0003-4815-5864

Project Number 6602c-ZF/18-231
Publication Date June 30, 2021
Submission Date February 24, 2021
Acceptance Date May 27, 2021
Published in Issue Year 2021 Volume: 5 Issue: 1

Cite

APA Endes, A. (2021). Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei. International Journal of Agriculture Forestry and Life Sciences, 5(1), 87-93.
AMA Endes A. Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei. Int J Agric For Life Sci. June 2021;5(1):87-93.
Chicago Endes, Ali. “Influence of Culture Media, Temperature, PH and Light Regime on Mycelial Growth of Ascochyta Rabiei”. International Journal of Agriculture Forestry and Life Sciences 5, no. 1 (June 2021): 87-93.
EndNote Endes A (June 1, 2021) Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei. International Journal of Agriculture Forestry and Life Sciences 5 1 87–93.
IEEE A. Endes, “Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei”, Int J Agric For Life Sci, vol. 5, no. 1, pp. 87–93, 2021.
ISNAD Endes, Ali. “Influence of Culture Media, Temperature, PH and Light Regime on Mycelial Growth of Ascochyta Rabiei”. International Journal of Agriculture Forestry and Life Sciences 5/1 (June 2021), 87-93.
JAMA Endes A. Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei. Int J Agric For Life Sci. 2021;5:87–93.
MLA Endes, Ali. “Influence of Culture Media, Temperature, PH and Light Regime on Mycelial Growth of Ascochyta Rabiei”. International Journal of Agriculture Forestry and Life Sciences, vol. 5, no. 1, 2021, pp. 87-93.
Vancouver Endes A. Influence of culture media, temperature, pH and light regime on mycelial growth of Ascochyta rabiei. Int J Agric For Life Sci. 2021;5(1):87-93.

download