Ahmed, B., Salahin, M., Karim, R., Razvy, M.A., Hannan, M.M., Sultana, R., Hossain and Islam, MR. 2007. An Efficient Method for in vitro Clonal Propagation of a Newly Introduced Sweetener Plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Scientific Research, 2 (2): 121-125.
Ahmed, M.B. and Salahin M. 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant Stevia rebaudiana in Bangladesh. American Journal of Scientific Research, 2(2): 121-125.
Brandle, J.E. and Rosa, N. 1992. Heritability for yield, leaf:stem ratio and stevioside content estimated from landrace cultivar of Stevia rebaudiana . Can. J. Plant Sci. 72: 1263-1266.
Debnath, M. 2008. Clonal propagation and antimicrobial activity of an endemic medicinal pant Stevia rebaudiana. J. of Medicinal Plants Research. 2, 045‐051.
Fatima, A. and Khan, S.J. 2010. Some factors affecting the in vitro growth of Stevia rebaudiana Bertoni. Iranian Journal of Plant Physiology 1 (2): 61‐68.
Felippe, G.M. and Lucas, N.M.C. 1971. Estudo da viabilidade dos frutos de Stevia rebaudiana Bert. Hoehnea 1:95 –105.
Ferreira. C.M. and Handro W. 1988. Micropropagation of Stevia rebaudiana through leaf explants from adult plants. Planta Medica. 54 (2): 157-160.
Flachsland, E. and Mroginski L. 1966. Regeneration of plants from anthers of Stevia rebaudiana Bertoni (Compositae) cultivated in vitro. Biocell. 20: 87-90.
George E.F. 1993. Plant propagation by tissue culture.Part - I. The Technology Exgetics Ltd., U.K.
Ghuari, E.G., A. Fatima, S. Ghani and N. Shafi. 2009. Micropropagation and antimicrobial activity of an exotic sweetener plant (Stevia rebaudiana Bertoni). Pak. J. Biochem. Mol. Biol., 42(2): 53-56.
Ibrahim, A., Mahmoud, I.N., Berlanti, R. and Mohammed M. 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Technology, 10(3): 254-259.
Katayama, O., Sumida, T., Hayashi, K. and Mitsuhashi, H. 1976. Safety of stevioside. In
Applicability of Stevia and Data on Research and Development, Isu Co. Ltd., Tokyo, pp. 225–281.
Kinghorn, D.A. 2002. Overview. In: Kinghorn, A.D. (Ed.), Stevia the Genus Stevia (Medicinal and Aromatic Plants – Industrial Profiles). Taylor & Francis/CRC Press, New York/London, UK, pp. 1-17.
Lu, C. 1993. The use of Thidiazuran in tissue culture. In vitro cell development. Biol. Plant., 29: 92-96.
Miyagawa, H. and Fujioka, N. 1986. Studies on the tissue culture of Stevia rebaudiana and its components: II. Induction of shoot primordia. Planta Medica. 4: 321-323.
Motomu, A., Shigeoka, T., Koizumi, Y., and Kawamura, M. 1994. Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Reports, 13 (3-4): 180-183.
Mousumi, D. 2008. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. Journal of Medicinal Plants Research, 2(2): 045-051.
Pourvi, J., Kachhwaha, S. and Kothari, SL. 2009. Improved micropropagation protocol and enhancement in biomass and chlorophyll content in Stevia rebaudiana (Bert.) Bertoni by using high copper levels in the culture medium. Scientia Horticulturae, 119(3): 315-319.
Rafiq, M., Muhammad, UD., Sher, MM., Naqvi, HA and Qarshi, IA. 2007. In Vitro Clonal Propagation and Biochemical Analysis of Field Established Stevia rebaudiana Bertoni. Pakistanian Journal of Botany, 39(7): 2467-2474.
Sakaguchi, M , and Kan, T. 1982. Japanese researches on Stevia rebaudiana (Bert.) Bertoni and stevioside. CiCult 34: 235-248.
Sivaram, L. and Mukundan, U. 2003. In vitro culture studies on Stevia rebaudiana. In vitro cell and developmental Biolology in Plant, 39: 520-523.
Sreedhar, R.V., Venkatachalam, R., Thimmaraju, N., Bhagyalakshmi, MS., Narayan, L. and Ravishankar, GA. 2008. Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum, 52 (2): 355-360.
Strauss,S. 1995. The perfect sweetener? Technol. Rev. 98: 18 20.
Tamura,Y., Nakamura,S., Fukui,H. and Tabata, M. 1984a. Comparison of Stevia plants grown from seeds, cuttings and stem tip cultures for growth and sweet diterpene glycosides. Plant Cell Rep. 3 : 180 182.
Tamura, Y., Nakamura, S., Fukui, H. and Tabata M. 1984b. Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture Plant Cell Reports 3: 183.
Tiwari, S., Arnold, R., Saxena, A., Mishra, R.M., Tiwarii A.S., Rajak, A. and Singh, P. 2013. Studies on rapid micropropagation of Stevia rebaudiana Bertoni: A natural sweetener. Int. J. of Pharm. & Life Sci. (IJPLS), Vol. 4, Issue 5: May: 2013, 2667-2671.
Toffler F. and Orio O.A. 1981 . Acceni sulla pin ata tropicale ‘Kaa – he - e’ ou ‘erba dolce’. Rev. Soc. Sci. Aliment 4: 225 - 230
Yang, Y.W., Y.I. Hsing and W.C. Chang. 1981. Clonal propagation of Stevia rebaudiana Bertoni through axillary shoot proliferation In vitro. Bot. Bull. Academia, 22: 57-62.
In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants
In Vitro Multiplication
of Stevia rebaudiana (Bertoni)
Genotypes by Using Different Explants
Fatih Seyis1, Emine
Yurteri1, Aysel Özcan1
1Recep Tayyip Erdogan University, Faculty of Agronomy
and Natural Sciences, Field Crops Department
Stevia
rebaudiana Bertoni
that is a member of the Compositae family is one of the most valuable tropical medicinal plants. The origin of Stevia is South America, where it can
be seen as a wild plant. Further it could be found in semi-arid habitat
ranging from grassland to scrub forest to mountain terrain. Conventional
cultivation or propagation methods of Stevia are as well known for time consuming, unpredictable, unreliable and less
productive. Therefore, there is a crucial need to develop methods for rapid
multiplication of this valuable plant. Plantlet produced through stem cutting
has unstability, however the
micropropagation of Stevia which
may overcome some limitations associated with conventional method can be used
for a rapid multiplication. Accordingly, the study results showed that
different explants such as leaf, stem and root transferred to different
tissue culture media for in vitro
multiplication of two Stevia rebaudiana
(Bertoni) genotypes could be reproduced by using different explants.
Key
words:Stevia rebaudiana, tissue culture,
multiplication, in vitro, genotype,
explant
Ahmed, B., Salahin, M., Karim, R., Razvy, M.A., Hannan, M.M., Sultana, R., Hossain and Islam, MR. 2007. An Efficient Method for in vitro Clonal Propagation of a Newly Introduced Sweetener Plant (Stevia rebaudiana Bertoni.) in Bangladesh. American-Eurasian Journal of Scientific Research, 2 (2): 121-125.
Ahmed, M.B. and Salahin M. 2007. An efficient method for in vitro clonal propagation of a newly introduced sweetener plant Stevia rebaudiana in Bangladesh. American Journal of Scientific Research, 2(2): 121-125.
Brandle, J.E. and Rosa, N. 1992. Heritability for yield, leaf:stem ratio and stevioside content estimated from landrace cultivar of Stevia rebaudiana . Can. J. Plant Sci. 72: 1263-1266.
Debnath, M. 2008. Clonal propagation and antimicrobial activity of an endemic medicinal pant Stevia rebaudiana. J. of Medicinal Plants Research. 2, 045‐051.
Fatima, A. and Khan, S.J. 2010. Some factors affecting the in vitro growth of Stevia rebaudiana Bertoni. Iranian Journal of Plant Physiology 1 (2): 61‐68.
Felippe, G.M. and Lucas, N.M.C. 1971. Estudo da viabilidade dos frutos de Stevia rebaudiana Bert. Hoehnea 1:95 –105.
Ferreira. C.M. and Handro W. 1988. Micropropagation of Stevia rebaudiana through leaf explants from adult plants. Planta Medica. 54 (2): 157-160.
Flachsland, E. and Mroginski L. 1966. Regeneration of plants from anthers of Stevia rebaudiana Bertoni (Compositae) cultivated in vitro. Biocell. 20: 87-90.
George E.F. 1993. Plant propagation by tissue culture.Part - I. The Technology Exgetics Ltd., U.K.
Ghuari, E.G., A. Fatima, S. Ghani and N. Shafi. 2009. Micropropagation and antimicrobial activity of an exotic sweetener plant (Stevia rebaudiana Bertoni). Pak. J. Biochem. Mol. Biol., 42(2): 53-56.
Ibrahim, A., Mahmoud, I.N., Berlanti, R. and Mohammed M. 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Technology, 10(3): 254-259.
Katayama, O., Sumida, T., Hayashi, K. and Mitsuhashi, H. 1976. Safety of stevioside. In
Applicability of Stevia and Data on Research and Development, Isu Co. Ltd., Tokyo, pp. 225–281.
Kinghorn, D.A. 2002. Overview. In: Kinghorn, A.D. (Ed.), Stevia the Genus Stevia (Medicinal and Aromatic Plants – Industrial Profiles). Taylor & Francis/CRC Press, New York/London, UK, pp. 1-17.
Lu, C. 1993. The use of Thidiazuran in tissue culture. In vitro cell development. Biol. Plant., 29: 92-96.
Miyagawa, H. and Fujioka, N. 1986. Studies on the tissue culture of Stevia rebaudiana and its components: II. Induction of shoot primordia. Planta Medica. 4: 321-323.
Motomu, A., Shigeoka, T., Koizumi, Y., and Kawamura, M. 1994. Mass propagation of shoots of Stevia rebaudiana using a large scale bioreactor. Plant Cell Reports, 13 (3-4): 180-183.
Mousumi, D. 2008. Clonal propagation and antimicrobial activity of an endemic medicinal plant Stevia rebaudiana. Journal of Medicinal Plants Research, 2(2): 045-051.
Pourvi, J., Kachhwaha, S. and Kothari, SL. 2009. Improved micropropagation protocol and enhancement in biomass and chlorophyll content in Stevia rebaudiana (Bert.) Bertoni by using high copper levels in the culture medium. Scientia Horticulturae, 119(3): 315-319.
Rafiq, M., Muhammad, UD., Sher, MM., Naqvi, HA and Qarshi, IA. 2007. In Vitro Clonal Propagation and Biochemical Analysis of Field Established Stevia rebaudiana Bertoni. Pakistanian Journal of Botany, 39(7): 2467-2474.
Sakaguchi, M , and Kan, T. 1982. Japanese researches on Stevia rebaudiana (Bert.) Bertoni and stevioside. CiCult 34: 235-248.
Sivaram, L. and Mukundan, U. 2003. In vitro culture studies on Stevia rebaudiana. In vitro cell and developmental Biolology in Plant, 39: 520-523.
Sreedhar, R.V., Venkatachalam, R., Thimmaraju, N., Bhagyalakshmi, MS., Narayan, L. and Ravishankar, GA. 2008. Direct organogenesis from leaf explants of Stevia rebaudiana and cultivation in bioreactor. Biologia Plantarum, 52 (2): 355-360.
Strauss,S. 1995. The perfect sweetener? Technol. Rev. 98: 18 20.
Tamura,Y., Nakamura,S., Fukui,H. and Tabata, M. 1984a. Comparison of Stevia plants grown from seeds, cuttings and stem tip cultures for growth and sweet diterpene glycosides. Plant Cell Rep. 3 : 180 182.
Tamura, Y., Nakamura, S., Fukui, H. and Tabata M. 1984b. Clonal propagation of Stevia rebaudiana Bertoni by stem-tip culture Plant Cell Reports 3: 183.
Tiwari, S., Arnold, R., Saxena, A., Mishra, R.M., Tiwarii A.S., Rajak, A. and Singh, P. 2013. Studies on rapid micropropagation of Stevia rebaudiana Bertoni: A natural sweetener. Int. J. of Pharm. & Life Sci. (IJPLS), Vol. 4, Issue 5: May: 2013, 2667-2671.
Toffler F. and Orio O.A. 1981 . Acceni sulla pin ata tropicale ‘Kaa – he - e’ ou ‘erba dolce’. Rev. Soc. Sci. Aliment 4: 225 - 230
Yang, Y.W., Y.I. Hsing and W.C. Chang. 1981. Clonal propagation of Stevia rebaudiana Bertoni through axillary shoot proliferation In vitro. Bot. Bull. Academia, 22: 57-62.
Seyis, F., Yurteri, E., & Özcan, A. (2017). In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants. International Journal of Crop Science and Technology, 3(2), 36-41. https://doi.org/10.26558/ijcst.326792
AMA
Seyis F, Yurteri E, Özcan A. In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants. IJCST. July 2017;3(2):36-41. doi:10.26558/ijcst.326792
Chicago
Seyis, Fatih, Emine Yurteri, and Aysel Özcan. “In Vitro Multiplication of Stevia Rebaudiana (Bertoni) Genotypes Using Different Explants”. International Journal of Crop Science and Technology 3, no. 2 (July 2017): 36-41. https://doi.org/10.26558/ijcst.326792.
EndNote
Seyis F, Yurteri E, Özcan A (July 1, 2017) In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants. International Journal of Crop Science and Technology 3 2 36–41.
IEEE
F. Seyis, E. Yurteri, and A. Özcan, “In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants”, IJCST, vol. 3, no. 2, pp. 36–41, 2017, doi: 10.26558/ijcst.326792.
ISNAD
Seyis, Fatih et al. “In Vitro Multiplication of Stevia Rebaudiana (Bertoni) Genotypes Using Different Explants”. International Journal of Crop Science and Technology 3/2 (July 2017), 36-41. https://doi.org/10.26558/ijcst.326792.
JAMA
Seyis F, Yurteri E, Özcan A. In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants. IJCST. 2017;3:36–41.
MLA
Seyis, Fatih et al. “In Vitro Multiplication of Stevia Rebaudiana (Bertoni) Genotypes Using Different Explants”. International Journal of Crop Science and Technology, vol. 3, no. 2, 2017, pp. 36-41, doi:10.26558/ijcst.326792.
Vancouver
Seyis F, Yurteri E, Özcan A. In Vitro Multiplication of Stevia rebaudiana (Bertoni) Genotypes Using Different Explants. IJCST. 2017;3(2):36-41.