Research Article

2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes

Number: 10 June 14, 2026
EN

2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes

Abstract

Glutathione, a thiol molecule, is necessary for healthy cells against oxidative stress and xenobiotics. Two glutathione-related enzymes glutathione reductase (GR) and glutathione S-transferase (GST) were studied in this paper. This study aimed to investigate the inhibitory potencies of 2-quinolinone derivatives on hGR and hGST and to reveal their structure–activity relationships by both kinetic and computational approaches. For this purpose, firstly, GST and GR were collected from human erythrocytes with specific activities of 8.78 EU/mg protein and 5.85 EU/mg protein. Then, inhibitory potencies of 2-quinolinone derivatives on hGR and hGST were assayed in vitro. IC50-[inhibitor] and Lineweaver-Burk graphs were generated. QU3, 6-hydroxy-2(h)-quinolinone, was found to be the most effective inhibitor against two enzymes with Ki values of 5.18±1.57 µM and 27.69±5.412 µM. Besides, binding energies of QU3 on hGR and hGST receptors were estimated as -5.59 kcal/mol and -5.81 kcal/mol. These findings suggest that the 2-quinolinone structure is a promising pharmacophore for use in new hGR and hGST assays. Future configuration modifications with higher selectivity and demonstrated efficacy are planned

Keywords

Supporting Institution

Atatürk University-Scientific Research Projects provided funding for this work (Grant Number: FYL-2025-15363).

Project Number

FYL-2025-15363

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Publication Date

June 14, 2026

Submission Date

December 3, 2025

Acceptance Date

January 5, 2026

Published in Issue

Year 2026 Number: 10

APA
Körükmez, M., & Güller, P. (2026). 2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes. International Journal of Chemistry and Technology, 10, 1-9. https://doi.org/10.32571/ijct.1835534
AMA
1.Körükmez M, Güller P. 2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes. Int. J. Chem. Technol. 2026;(10):1-9. doi:10.32571/ijct.1835534
Chicago
Körükmez, Mesut, and Pınar Güller. 2026. “2-Quinolinone Derivatives As Potential Inhibitors of Glutathione-Related Enzymes”. International Journal of Chemistry and Technology, nos. 10: 1-9. https://doi.org/10.32571/ijct.1835534.
EndNote
Körükmez M, Güller P (June 1, 2026) 2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes. International Journal of Chemistry and Technology 10 1–9.
IEEE
[1]M. Körükmez and P. Güller, “2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes”, Int. J. Chem. Technol., no. 10, pp. 1–9, June 2026, doi: 10.32571/ijct.1835534.
ISNAD
Körükmez, Mesut - Güller, Pınar. “2-Quinolinone Derivatives As Potential Inhibitors of Glutathione-Related Enzymes”. International Journal of Chemistry and Technology. 10 (June 1, 2026): 1-9. https://doi.org/10.32571/ijct.1835534.
JAMA
1.Körükmez M, Güller P. 2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes. Int. J. Chem. Technol. 2026;:1–9.
MLA
Körükmez, Mesut, and Pınar Güller. “2-Quinolinone Derivatives As Potential Inhibitors of Glutathione-Related Enzymes”. International Journal of Chemistry and Technology, no. 10, June 2026, pp. 1-9, doi:10.32571/ijct.1835534.
Vancouver
1.Mesut Körükmez, Pınar Güller. 2-Quinolinone Derivatives as Potential Inhibitors of Glutathione-Related Enzymes. Int. J. Chem. Technol. 2026 Jun. 1;(10):1-9. doi:10.32571/ijct.1835534