Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yakup Akkoç; S. Seyhan Tükel

doi:10.32571/ijct.420772

TR EN

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Abstract

In this study, in order to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and glucose isomerase (GI) were immobilized together with cross-linked enzyme aggregates (CLEAs), and named as 'combi-CLEAs'. The values of V_maxand K_mfrom the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12 U g^-1 and 0.1157 mg ml^-1 in optimum conditions, respectively. After 50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL + GI] was preserved at 46%. The thermal stability of combi-CLEAs [GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C after 24 hours. At the end of 30 days, the storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and 13% at the temperatures of 4 and 25°C, respectively.

Keywords

Combi-CLEAs,fructose syrup,enzyme immobilization

References

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Details

Primary Language

English

Subjects

Chemical Engineering

Journal Section

Research Article

Authors

Yakup Akkoç ^*
0000-0002-2622-1341
Türkiye

S. Seyhan Tükel This is me
0000-0003-2503-2781
Türkiye

Publication Date

June 27, 2018

Submission Date

May 3, 2018

Acceptance Date

June 10, 2018

Published in Issue

Year 2018 Volume: 2 Number: 1

DOI

https://doi.org/10.32571/ijct.420772

IZ

https://izlik.org/JA27GK96UP

Cite

RIS / Bibtex

APA

Akkoç, Y., & Tükel, S. S. (2018). Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology, 2(1), 63-67. https://doi.org/10.32571/ijct.420772

AMA

1.Akkoç Y, Tükel SS. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. 2018;2(1):63-67. doi:10.32571/ijct.420772

Chicago

Akkoç, Yakup, and S. Seyhan Tükel. 2018. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology 2 (1): 63-67. https://doi.org/10.32571/ijct.420772.

EndNote

Akkoç Y, Tükel SS (June 1, 2018) Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology 2 1 63–67.

IEEE

[1]Y. Akkoç and S. S. Tükel, “Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step”, Int. J. Chem. Technol., vol. 2, no. 1, pp. 63–67, June 2018, doi: 10.32571/ijct.420772.

ISNAD

Akkoç, Yakup - Tükel, S. Seyhan. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology 2/1 (June 1, 2018): 63-67. https://doi.org/10.32571/ijct.420772.

JAMA

1.Akkoç Y, Tükel SS. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. 2018;2:63–67.

MLA

Akkoç, Yakup, and S. Seyhan Tükel. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology, vol. 2, no. 1, June 2018, pp. 63-67, doi:10.32571/ijct.420772.

Vancouver

1.Yakup Akkoç, S. Seyhan Tükel. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. 2018 Jun. 1;2(1):63-7. doi:10.32571/ijct.420772