Recombinant human G-CSF production as a protein based drug candidate for hematology and oncology

Year 2019, Volume: 3 Issue: 2, 92 - 100, 31.12.2019

Yasemin Bozkurt Sema Bilgin , Seçil Erden , İsmail Furkan Turan , İsa Gökçe

https://doi.org/10.32571/ijct.600180

Cited By: 2

https://izlik.org/JA84NT99CS

Abstract

The human granulocyte colony stimulating factor
(hG-CSF) is a member of the CSF family. The first purpose of this study was production of
recombinant human G-CSF (rhG-CSF) which is an important therapeutic protein for
angiogenesis based clinical applicaitons. rhG-CSF was produced as
inclusion body in the Escherichia coli
strain BL21 (DE3) pLysE with pTOLT vector system. The rhG-CSF was purified by
Ni-NTA agarose afinity chromatography and characterized with SDS-PAGE analysis.
The effects of this therapeutic protein on cell
viability was measured by MTT assay. Additionally,  angiogenic potential of produced rhG-CSF
was investigated via HUVEC cell line by in vitro scratch assay.  As
a result, the purified protein induced cell proliferation and the EC50 value of protein based drug candidate was 0.051 mM. Additionally, it was determined that the migration ability of
the HUVECs was promoted by rhG-CSF in a concentration-dependent manner by in vitro scratch assay.  

Keywords

Granulocyte colony stimulating factor , recombinant proteins , angiogenesis , cell proliferation

Supporting Institution

Tokat Gaziosmanpasa University Scientific Research Project

Project Number

2016/61

Thanks

This study was financially supported by the Tokat Gaziosmanpasa University Scientific Research Project (2016/61). Additionally, Yasemin BOZKURT was supported by TUBİTAK-BİDEB 2210/C National Scholarship Program for MSc students.

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