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Year 2018, Volume: 2 Issue: 1, 63 - 67, 27.06.2018

Yakup Akkoç S. Seyhan Tükel

https://doi.org/10.32571/ijct.420772

Abstract


Keywords

Combi-CLEA HFS Enzyme immobilization

References

  • 1. Schindler, I.; Renz, A.; Schmid, F.X.;Beck, E. Biochım. Biophys. Acta 2001,1548, 175-186.
  • 2. Cha, X.; Zhou, H.; Li, W.; Shen, J. J. Chem. Technol. Biot. 1990, 47, 161-169.
  • 3. Schafhauser, D.Y.; Storey, K.B. Appl. Biochem. Biotechnol. 1992, 36, 63-74.
  • 4. Yubin,Ge.; Yingming, W.; Hui, Zhou, S. W.; Yi, T.; Wei, L. J. Biotechnol. 1999, 67, 33-34.
  • 5. Sohel, D.; Manali, K.; Munishwar, N. G. J. Mol. Catal. B-Enzym. 2007, 44, 128-132.
  • 6. Sachin, T.; Amol, P.; Mayur, L.; Shamraja, N.; Mosin, M.; Kshitija, J.; Kishori,P.; Devika, A. Bioresour. Technol. 2013, 147, 269-275.
  • 7. Jolanta, B. Biochem. Eng. J. 2003,16, 347-355.
  • 8. Qiaolin, L.; Long, Y.; Jianguo, S.; Liang, L.; Lin, X.; Aihua, Li. Biosens.. Bioelectron. .2014, 51, 158-163.
  • 9. Gupta, M.N.; Dalal, S.; Kapoor, M. J. Mol. Catal. Enzyme B. 2017, 44, 128-132.
  • 10. Tolsma, A. D. The effects of fire and grazing on the energy reserves of resprouting plants in Victoria's alpine grasslands. PhD Thesis, School of Resource Management, Forestry and Amenity Horticulture, The University of Melbourne, 2002.
  • 11. Roushdi, M.; Ghali, Y.; Attia, R. M.; Alaa El-din, M. Starch/Stärke. 1979, 31 (12), 414-417.
  • 12. Katwa, L.C.: Raghavendra, M.R. Technol. Lett. 1983, 5, 191-196.
  • 13. Mıshra, A.; Debnath, M. App. Biochem. Biotechnol. 2002, 102-103.
  • 14. Michaelis, L.; Menten, M. Biochem. Zeitung, 1913, 49, 333-369.

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Year 2018, Volume: 2 Issue: 1, 63 - 67, 27.06.2018

Yakup Akkoç S. Seyhan Tükel

https://doi.org/10.32571/ijct.420772

Abstract

In this study, in order
to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and
glucose isomerase (GI) were immobilized together with cross-linked enzyme
aggregates (CLEAs), and named as 'combi-CLEAs'. The values of Vmaxand Kmfrom
the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12      U g-1 and 0.1157 mg ml-1
in optimum conditions, respectively. After
50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL +
GI] was preserved at 46%. The thermal stability of combi-CLEAs
[GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C
after 24 hours. At the end of 30 days, the
storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and
13% at the temperatures of 4 and 25°C, respectively.

Keywords

Combi-CLEAs fructose syrup enzyme immobilization

References

  • 1. Schindler, I.; Renz, A.; Schmid, F.X.;Beck, E. Biochım. Biophys. Acta 2001,1548, 175-186.
  • 2. Cha, X.; Zhou, H.; Li, W.; Shen, J. J. Chem. Technol. Biot. 1990, 47, 161-169.
  • 3. Schafhauser, D.Y.; Storey, K.B. Appl. Biochem. Biotechnol. 1992, 36, 63-74.
  • 4. Yubin,Ge.; Yingming, W.; Hui, Zhou, S. W.; Yi, T.; Wei, L. J. Biotechnol. 1999, 67, 33-34.
  • 5. Sohel, D.; Manali, K.; Munishwar, N. G. J. Mol. Catal. B-Enzym. 2007, 44, 128-132.
  • 6. Sachin, T.; Amol, P.; Mayur, L.; Shamraja, N.; Mosin, M.; Kshitija, J.; Kishori,P.; Devika, A. Bioresour. Technol. 2013, 147, 269-275.
  • 7. Jolanta, B. Biochem. Eng. J. 2003,16, 347-355.
  • 8. Qiaolin, L.; Long, Y.; Jianguo, S.; Liang, L.; Lin, X.; Aihua, Li. Biosens.. Bioelectron. .2014, 51, 158-163.
  • 9. Gupta, M.N.; Dalal, S.; Kapoor, M. J. Mol. Catal. Enzyme B. 2017, 44, 128-132.
  • 10. Tolsma, A. D. The effects of fire and grazing on the energy reserves of resprouting plants in Victoria's alpine grasslands. PhD Thesis, School of Resource Management, Forestry and Amenity Horticulture, The University of Melbourne, 2002.
  • 11. Roushdi, M.; Ghali, Y.; Attia, R. M.; Alaa El-din, M. Starch/Stärke. 1979, 31 (12), 414-417.
  • 12. Katwa, L.C.: Raghavendra, M.R. Technol. Lett. 1983, 5, 191-196.
  • 13. Mıshra, A.; Debnath, M. App. Biochem. Biotechnol. 2002, 102-103.
  • 14. Michaelis, L.; Menten, M. Biochem. Zeitung, 1913, 49, 333-369.
There are 14 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Research Articles
Authors

Yakup Akkoç 0000-0002-2622-1341

S. Seyhan Tükel This is me 0000-0003-2503-2781

Publication Date June 27, 2018
Published in Issue Year 2018 Volume: 2 Issue: 1

Cite

APA Akkoç, Y., & Tükel, S. S. (2018). Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology, 2(1), 63-67. https://doi.org/10.32571/ijct.420772
AMA Akkoç Y, Tükel SS. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. June 2018;2(1):63-67. doi:10.32571/ijct.420772
Chicago Akkoç, Yakup, and S. Seyhan Tükel. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology 2, no. 1 (June 2018): 63-67. https://doi.org/10.32571/ijct.420772.
EndNote Akkoç Y, Tükel SS (June 1, 2018) Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. International Journal of Chemistry and Technology 2 1 63–67.
IEEE Y. Akkoç and S. S. Tükel, “Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step”, Int. J. Chem. Technol., vol. 2, no. 1, pp. 63–67, 2018, doi: 10.32571/ijct.420772.
ISNAD Akkoç, Yakup - Tükel, S. Seyhan. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology 2/1 (June 2018), 63-67. https://doi.org/10.32571/ijct.420772.
JAMA Akkoç Y, Tükel SS. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. 2018;2:63–67.
MLA Akkoç, Yakup and S. Seyhan Tükel. “Co-Immobilization of Pullulanase, Glucoamylase and Glucose Isomerase Together With Cross-Linked Enzyme Aggregates: Fructose Conversion from Three Enzymes and Starch in One Step”. International Journal of Chemistry and Technology, vol. 2, no. 1, 2018, pp. 63-67, doi:10.32571/ijct.420772.
Vancouver Akkoç Y, Tükel SS. Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step. Int. J. Chem. Technol. 2018;2(1):63-7.
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