Inhibition effects of commonly used some antibacterial and antiviral drugs on purified human serum paraoxonase-1 (hPON1)

Year 2019, Volume: 3 Issue: 2, 146 - 150, 31.12.2019

Hakan Soyut

https://doi.org/10.32571/ijct.651334

Abstract

hPON1 is an enzyme from the group of A-esterases which
is capable of hydrolyzing the active metabolite paraoxon of parathion, an
organic phosphorus insecticide. It is an important liver enzyme that plays a
protective role against the hydrolysis of HDL-induced organophosphate agents
and nerve gases, oxidation of LDL, formation of lipid peroxides and bacterial
endotoxins. The fact that oxidation of LDL constitutes the initial stage of the
atherosclerosis process reveals the importance of the antioxidant properties of
the enzyme. In this study, human serum PON1 was purified using three simple
biochemical purification techniques. Furthermore, the in vitro effects of some
antibacterial and antiviral drugs on human serum PON1 enzyme activity were
examined. IC50 values were determined.

Keywords

Paraoxonase, enzyme inhibition, antibacterial drug, antiviral drug

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