Research Article
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Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive

Year 2022, Volume: 6 Issue: 1, 33 - 38, 06.07.2022

Canan Gülmez , Onur Atakisi

https://doi.org/10.32571/ijct.1085417

Abstract

Herein, it is aimed to perform some commercial liquid and solid laundry detergents, surfactants, oxidizing agent and pH/temperature studies of lipase from porcine pancreas. For this, optimum pH and temperatures of lipase were investigated in the range of pH 5-13 and temperature 30-80°C. The enzyme stability and compatibility were evaluated in the presence of 8 commercial laundry detergents, triton X-100, tween 20, tween 80, tergitol, sodium deoxycolate, sodium dodecyl sulfate and oxidizing agent H202 for 1 h at 40°C. Optimum pH and temperatures of enzymes was recorded pH 10 and 40°C, respectively. The findings revealed that lipase was generally stable in detergents and increased by more than 20% in liquid detergent 1 and 3 compared to the control. In addition, enzyme in triton X-100, tween 20, tween 80, and tergitol at 5%(v/v) concentration exhibited about 237%, 281%, 207% and 237% relative activities and activity at 5% H202(v/v) was inhibited by only about 13%. Consequently, pancreatic lipase can be a good choice in enzyme immobilization studies and various biotechnological purposes, especially in detergent applications.

Keywords

Lipase laundry detergent surfactant bleaching agent detergent industry

References

  • Mendes, A.A.; Oliveira, P.C.; de Castro, H. F. J. Mol. Catal. B Enzym. 2012, 78, 119-134.
  • Contesini, F.J.; Lopes, D. B.; Macedo, G. A.; da Graça Nascimento, M.; de Oliveira Carvalho, P. J. Mol. Catal. B Enzym. 2010, 67(3-4), 163-171.
  • Javed, S.; Azeem, F.; Hussain, S.; Rasul, I.; Siddique, M.H.; Riaz, M.; Afzal, M.; Kouser, A. Nadeem, H. Prog. Biophys. Mol. Biol. 2018, 132, 23-34.
  • Ray, A. Asian J. Pharm. Sci. 2012, 2(2), 33-37.
  • Al-Ghanayem, A.A.; Joseph, B. Appl. Microbiol. Biotechnol. 2020, 104(7), 2871-2882.
  • Houde, A.; Kademi, A.; Leblanc, D. Appl. Biochem. Biotechnol. 2004, 118(1), 155-170.
  • Contesini, F.J.; Davanço, M.G.; Borin, G.P.; Vanegas, K.G.; Cirino, J.P.G.; Melo, R.R.D.; Mortensen, U.E.; Hildén, K.; Campos, D.R.; Carvalho, P.D.O. Catalysts, 2020, 10(9), 1032.
  • Gulmez, C.; Atakisi, O.; Dalginli, K.Y.; Atakisi, E. Int. J. Biol. Macromol. 2018, 108, 436-443.
  • Abol-Fotouh, D.; AlHagar, O.E.; Hassan, M.A. Int. J. Biol. Macromol. 2021, 181, 125-135.
  • Jaeger, K.E.; Ransac, S.; Dijkstra, B.W.; Colson, C.; Heuvel, M.; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29-63.
  • Bernal, C.; Rodriguez, K.; Martinez, R. Biotechnol. Adv. 2018, 36(5), 1470-1480.
  • Altinkaynak, C.; Gulmez, C.; Atakisi, O.; Ozdemir, N. Int. J. Biol. Macromol. 2020, 164, 162-171.
  • Almeida, F.L.; Prata, A.S.; Forte, M.B. Biofuels Bioprod. Biorefining. 2021, 16, 587-608.
  • Vasconcelos, A.; Silva, C.J.; Schroeder, M.; Guebitz, G.M.; Cavaco-Paulo, A. Biotechnol. Lett. 2006, 28(10), 725-731.
  • Minteer, S.D. Cell-free biotechnologies. 2006, 433-448.
  • Winkler, U.K.; Stuckman, M. J. Bacteriol. Res. 1979, 138, 663-70.
  • Bradford, M.M. Anal. Biochem. 1976, 72, 248-252.
  • Rahman, R.; Geok, L.; Basri, M.; Salleh, A. Enzyme Microb. Technol. 2006, 39, 1484–1491.
  • Phukon, L.C.; Chourasia, R.; Kumari, M.; Godan, T.K.; Sahoo, D.; Parameswaran, B.; Rai, A.K. Bioresour. Technol. 2020, 309, 123352.
  • Akmoussi-Toumi, S.; Khemili-Talbi, S.; Ferioune, I.; Kebbouche-Gana, S. Int. J. Biol. Macromol. 2018, 116, 817-830.
  • Hasan, F.; Shah, A.A.; Javed, S.; Hameed, A. Afr. J. Biotechnol. 2010, 9(31), 4836-4844.
  • Gurkok, S.; Ozdal, M. Protein Expr. Purif. 2021, 180, 105819.
  • Zhao, J., Liu, S., Gao, Y., Ma, M., Yan, X., Cheng, D., Wan, D.; Zeng, Z.; Yu, P.; Gong, D. Int. J. Biol. Macromol. 2021, 176, 126-136.
  • Jurado, E.; Garcia-Roman, M.; Luzon, G.; Altmajer-Vaz, D.; Jiménez-Pérez, J.L. Ind. Eng. Chem. Res. 2011, 50(20), 11502-11510.
  • Saraswat, R.; Verma, V.; Sistla, S.; Bhushan, I. Electron. J. Biotechnol. 2017, 30, 33-38.
  • Grbavčić, S.; Bezbradica, D.; Izrael-Živković, L.; Avramović, N.; Milosavić, N.; Karadžić, I.; Knežević-Jugović, Z. Bioresour. Technol. 2011, 102(24), 11226-11233.
  • Daoud, L.; Kamoun, J.; Ali, M.B.; Jallouli, R.; Bradai, R.; Mechichi, T.; Gargouri, Y.; Ali, Y.B.; Aloulou, A. Int. J. Biol. Macromol. 2013, 57, 232-237.
  • Zadymova, N.M.; Yampol’skaya, G.P.; Filatova, L.Y. Colloid J. 2006, 68(2), 162-172.
  • Bajpai, D. J. Oleo Sci. 2007, 56(7), 327-340.
  • Carpenter, J.F.; Chang, B.S.; Garzon-Rodriguez, W.; Randolph, T.W. 2002, 109-133.
  • Santos, S.F.; Zanette, D.; Fischer, H.; Itri, R. J. Colloid Interface Sci. 2003, 262(2), 400-408.
  • Salihu, A.; Alam, M.Z. Solvent tolerant lipases: a review. Process Biochem. 2015, 50(1), 86-96.

Year 2022, Volume: 6 Issue: 1, 33 - 38, 06.07.2022

Canan Gülmez , Onur Atakisi

https://doi.org/10.32571/ijct.1085417

Abstract

Bu çalışmada domuz pankreasından elde edilen lipazın ticari sıvı ve katı deterjanlar, yüzey aktif maddeler, oksitleyici ajan ve pH/sıcaklık çalışmalarının gerçekleştirilmesi amaçlanmıştır. Bu amaçla enzimin optimum pH ve sıcaklık çalışmaları sırasıyla pH 5-13 ve sıcaklık 30-80°C aralıklarında araştırılmıştır. Enzimin 8 adet ticari çamaşır deterjanı, triton X-100, tween 20, tween 80, tergitol, sodyum deoksikolat, sodyum dodesil sülfat ve oksitleyici ajan H202 varlığında 1 saat ve 40°C’de stabilite ve uyumluluğu değerlendirildi. Elde edilen bulgulara göre lipaz deterjanlar içerisinde genel olarak stabildir ve kontrole göre sıvı deterjan 1 ve 3 içerisinde aktivitelerini %20’den fazla artırmıştır. Aynı zamanda enzim 5% (v/v) konsantrasyonda triton X-100, tween 20, tween 80, and tergitol varlığında sırasıyla yaklaşık 237%, 281%, 207% and 237% relative aktivite göstermiştir. Enzim aktivitesi %5 (v/v) H202 konsantrayonunda sadece yaklaşık %13 inhibe olmuştur. Sonuç olarak; pankreatik lipaz, enzim immobilizasyon çalışmaları ve özellikle deterjan uygulamaları olmak üzere çeşitli biyoteknolojik amaçlar için iyi bir seçenek olabilir.

Keywords

Lipaz çamaşır deterjanı yüzey aktif maddeler ağartıcı ajanlar deterjan endüstrisi

References

  • Mendes, A.A.; Oliveira, P.C.; de Castro, H. F. J. Mol. Catal. B Enzym. 2012, 78, 119-134.
  • Contesini, F.J.; Lopes, D. B.; Macedo, G. A.; da Graça Nascimento, M.; de Oliveira Carvalho, P. J. Mol. Catal. B Enzym. 2010, 67(3-4), 163-171.
  • Javed, S.; Azeem, F.; Hussain, S.; Rasul, I.; Siddique, M.H.; Riaz, M.; Afzal, M.; Kouser, A. Nadeem, H. Prog. Biophys. Mol. Biol. 2018, 132, 23-34.
  • Ray, A. Asian J. Pharm. Sci. 2012, 2(2), 33-37.
  • Al-Ghanayem, A.A.; Joseph, B. Appl. Microbiol. Biotechnol. 2020, 104(7), 2871-2882.
  • Houde, A.; Kademi, A.; Leblanc, D. Appl. Biochem. Biotechnol. 2004, 118(1), 155-170.
  • Contesini, F.J.; Davanço, M.G.; Borin, G.P.; Vanegas, K.G.; Cirino, J.P.G.; Melo, R.R.D.; Mortensen, U.E.; Hildén, K.; Campos, D.R.; Carvalho, P.D.O. Catalysts, 2020, 10(9), 1032.
  • Gulmez, C.; Atakisi, O.; Dalginli, K.Y.; Atakisi, E. Int. J. Biol. Macromol. 2018, 108, 436-443.
  • Abol-Fotouh, D.; AlHagar, O.E.; Hassan, M.A. Int. J. Biol. Macromol. 2021, 181, 125-135.
  • Jaeger, K.E.; Ransac, S.; Dijkstra, B.W.; Colson, C.; Heuvel, M.; Misset, O. FEMS Microbiol. Rev. 1994, 15, 29-63.
  • Bernal, C.; Rodriguez, K.; Martinez, R. Biotechnol. Adv. 2018, 36(5), 1470-1480.
  • Altinkaynak, C.; Gulmez, C.; Atakisi, O.; Ozdemir, N. Int. J. Biol. Macromol. 2020, 164, 162-171.
  • Almeida, F.L.; Prata, A.S.; Forte, M.B. Biofuels Bioprod. Biorefining. 2021, 16, 587-608.
  • Vasconcelos, A.; Silva, C.J.; Schroeder, M.; Guebitz, G.M.; Cavaco-Paulo, A. Biotechnol. Lett. 2006, 28(10), 725-731.
  • Minteer, S.D. Cell-free biotechnologies. 2006, 433-448.
  • Winkler, U.K.; Stuckman, M. J. Bacteriol. Res. 1979, 138, 663-70.
  • Bradford, M.M. Anal. Biochem. 1976, 72, 248-252.
  • Rahman, R.; Geok, L.; Basri, M.; Salleh, A. Enzyme Microb. Technol. 2006, 39, 1484–1491.
  • Phukon, L.C.; Chourasia, R.; Kumari, M.; Godan, T.K.; Sahoo, D.; Parameswaran, B.; Rai, A.K. Bioresour. Technol. 2020, 309, 123352.
  • Akmoussi-Toumi, S.; Khemili-Talbi, S.; Ferioune, I.; Kebbouche-Gana, S. Int. J. Biol. Macromol. 2018, 116, 817-830.
  • Hasan, F.; Shah, A.A.; Javed, S.; Hameed, A. Afr. J. Biotechnol. 2010, 9(31), 4836-4844.
  • Gurkok, S.; Ozdal, M. Protein Expr. Purif. 2021, 180, 105819.
  • Zhao, J., Liu, S., Gao, Y., Ma, M., Yan, X., Cheng, D., Wan, D.; Zeng, Z.; Yu, P.; Gong, D. Int. J. Biol. Macromol. 2021, 176, 126-136.
  • Jurado, E.; Garcia-Roman, M.; Luzon, G.; Altmajer-Vaz, D.; Jiménez-Pérez, J.L. Ind. Eng. Chem. Res. 2011, 50(20), 11502-11510.
  • Saraswat, R.; Verma, V.; Sistla, S.; Bhushan, I. Electron. J. Biotechnol. 2017, 30, 33-38.
  • Grbavčić, S.; Bezbradica, D.; Izrael-Živković, L.; Avramović, N.; Milosavić, N.; Karadžić, I.; Knežević-Jugović, Z. Bioresour. Technol. 2011, 102(24), 11226-11233.
  • Daoud, L.; Kamoun, J.; Ali, M.B.; Jallouli, R.; Bradai, R.; Mechichi, T.; Gargouri, Y.; Ali, Y.B.; Aloulou, A. Int. J. Biol. Macromol. 2013, 57, 232-237.
  • Zadymova, N.M.; Yampol’skaya, G.P.; Filatova, L.Y. Colloid J. 2006, 68(2), 162-172.
  • Bajpai, D. J. Oleo Sci. 2007, 56(7), 327-340.
  • Carpenter, J.F.; Chang, B.S.; Garzon-Rodriguez, W.; Randolph, T.W. 2002, 109-133.
  • Santos, S.F.; Zanette, D.; Fischer, H.; Itri, R. J. Colloid Interface Sci. 2003, 262(2), 400-408.
  • Salihu, A.; Alam, M.Z. Solvent tolerant lipases: a review. Process Biochem. 2015, 50(1), 86-96.
There are 32 citations in total.

Details

Primary Language English
Subjects Chemical Engineering
Journal Section Research Articles
Authors

Canan Gülmez 0000-0003-3253-1407

Onur Atakisi 0000-0003-1183-6076

Publication Date July 6, 2022
Published in Issue Year 2022 Volume: 6 Issue: 1

Cite

APA Gülmez, C., & Atakisi, O. (2022). Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive. International Journal of Chemistry and Technology, 6(1), 33-38. https://doi.org/10.32571/ijct.1085417
AMA Gülmez C, Atakisi O. Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive. Int. J. Chem. Technol. July 2022;6(1):33-38. doi:10.32571/ijct.1085417
Chicago Gülmez, Canan, and Onur Atakisi. “Investigation of Compatibility of Lipase With Commercial Detergents, Surfactants and Oxidizing Agent As a Detergent Additive”. International Journal of Chemistry and Technology 6, no. 1 (July 2022): 33-38. https://doi.org/10.32571/ijct.1085417.
EndNote Gülmez C, Atakisi O (July 1, 2022) Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive. International Journal of Chemistry and Technology 6 1 33–38.
IEEE C. Gülmez and O. Atakisi, “Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive”, Int. J. Chem. Technol., vol. 6, no. 1, pp. 33–38, 2022, doi: 10.32571/ijct.1085417.
ISNAD Gülmez, Canan - Atakisi, Onur. “Investigation of Compatibility of Lipase With Commercial Detergents, Surfactants and Oxidizing Agent As a Detergent Additive”. International Journal of Chemistry and Technology 6/1 (July 2022), 33-38. https://doi.org/10.32571/ijct.1085417.
JAMA Gülmez C, Atakisi O. Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive. Int. J. Chem. Technol. 2022;6:33–38.
MLA Gülmez, Canan and Onur Atakisi. “Investigation of Compatibility of Lipase With Commercial Detergents, Surfactants and Oxidizing Agent As a Detergent Additive”. International Journal of Chemistry and Technology, vol. 6, no. 1, 2022, pp. 33-38, doi:10.32571/ijct.1085417.
Vancouver Gülmez C, Atakisi O. Investigation of compatibility of lipase with commercial detergents, surfactants and oxidizing agent as a detergent additive. Int. J. Chem. Technol. 2022;6(1):33-8.
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