In this study, we aimed to investigate the apoptotic effects of the lanthanide complex TBK-23, which was used in biological imaging due to its magnetic and spectroscopic properties. The study utilized HTB-54 and BEAS-2B cell lines. The cells were subjected to TBK-23 and cisplatin at active doses for a duration of 24 hours. Subsequently, flow cytometry was employed to assess the quantity of apoptotic cells, while quantitative RT-PCR was used to measure the expression levels of BAX, BCL-2, and BCL-xL. While cisplatin at 31 µM increased the proapoptotic BAX expression level in HTB-54 and BEAS-2B cells, TBK-23 increased BAX in cancer cells at the same concentration, but there was no observable effect in healthy cells. TBK-23 was found to have higher inhibitory effect on the expression of the antiapoptotic BCL-2 gene in cancer cells compared to that of cisplatin-treated cells. Consequently, it was determined that TBK-23, a novel metal-based compound, exhibited apoptotic effects similar to that of cisplatin and could be a potential chemotherapeutic agent.
Gaziantep Üniversity
Respiratory Diseases and Respiratory Surgery Research and Practice Center, Gaziantep University
Primary Language | English |
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Subjects | Structural Biology |
Journal Section | Research Articles |
Authors | |
Early Pub Date | January 10, 2024 |
Publication Date | December 31, 2023 |
Published in Issue | Year 2023 Volume: 7 Issue: 2 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.