Determination of In Vitro Biocontrol Efficacy of Trichoderma Harzianum Against Some Wheat Pathogen Fusarium Species
Year 2024,
Volume: 8 Issue: 1, 69 - 72, 05.06.2024
Aydın Atakan
,
Vakkas Ataş
Abstract
Recently, phytopathogenic problems have been increasing day by day in cereal production and have adverse effects on its production. One of the most common methods applied to control phytopathogens is the use of synthetic chemicals. The impacts of pesticides on the environment and food safety have made their use controversial. Therefore, developing and applying methods that can replace agricultural chemicals is an issue that needs to be emphasized. In this sense, biocontrol can be an alternative approach against agrochemicals in terms of sustainable agriculture. In the present study, it was aimed to examine the biocontrol efficiency of Trichoderma harzianum against Fusarium graminearum, Fusarium culmorum and Fusarium avenaceum, causing product losses in wheat by dual culture tests and double plate assays. Results showed that T. harzianum had remarkable inhibition of mycelial growth of all Fusarium species in dual culture assays. Moreover, no statistically crucial differences were found in the double plate tests.
Supporting Institution
Gaziantep University
Project Number
AMYO.ÖKAP.22.01.
Thanks
We are grateful to the Gaziantep University Scientific Research Projects Coordination Unit for their financial support for project numbered AMYO.ÖKAP.22.01. We also thank Assoc. Prof. Dr. Emre YÖRÜK for providing fungal isolates.
References
- 1. Henriksen, B.; Elen, O. J Phytopathol. 2005, 153, 214–220.
- 2. Srivastava, S.K; Huang, X; Brar, H.K.; Fakhoury, A.M.; Bluhm, B.H,; Bhattacharyya, M.K. Plos One. 2014, 9(1), e81832.
- 3. Lanubile, A.; Maschietto, V.; Borrelli, V.M.; Stagnati, L.; Logrieco, A.F.; Marocco, A. Front Plant Sci. 2017, 8, 1774.
- 4. Tian, Y.; Tan, Y.; Yan, Z.; Liao, Y.; Chen, J.; De Boevre, M. Front Microbiol. 2018, 8, 2710.
- 5. Shi, W.; Tan, Y.; Wang, S.; Gardiner, D.M.; De Saeger, S.; Liao, Y.; Wu, A. Toxins. 2016, 9(1), 6.
- 6. Rojas, E.C.; Jørgensen, H.J.; Jensen, B.; Collinge, D.B. Fusarium diseases: biology and management perspectives, 1st ed.; Burleigh Dodds Science Publishing, Cambridge, 2018.
- 7. Wegulo, S.N.; Baenziger, P.S.; Nopsa, J.H.; Bockus, W.W.; Hallen-Adams, H. Crop Prot. 2015, 73, 100-107.
- 8. El-Sobky, M.A.; Fahmi, A.I.; Eissa, R.A.; El-Zanaty, A.M. J Microb Biochem Technol. 2019, 11(1).
- 9. Arora, N.K.; Fatima, T.; Mishra, I.; Verma, S. Microbe-based inoculants: role in next green revolution. In Environmental concerns and sustainable development: Shakla, V., Ed.; Springer, Singapore, 2020; pp. 191-246.
- 10. Troian, R.F.; Steindorff, A.S.; Ramada, M.H.S.; Arruda, W.; Ulhoa, C.J. Biotechnol Lett. 2014, 36, 2095-2101.
11. Martínez-Medina, A.; Del Mar Alguacil, M.; Pascual, J.A.; Van Wees, S., J Chem Ecol. 2014, 40(7), 804-815.
- 12. Li, M.F.; Li, G.H.; Zhang, K.Q. Metabolites. 2019, 9(3), 58.
- 13. Sarrocco, S.; Esteban, P.; Vicente, I.; Bernardi, R.; Plainchamp, T.; Domenichini, S.; Dufresne, M. Phytopathology. 2021, 111(7), 1129-1136.
- 14. Alfiky, A.; Weisskopf, L. J Fungi. 2021, 7(1), 61.
- 15. Zeilinger, S.; Gruber, S.; Bansal, R.; Mukherjee, P.K. Fungal Biol Rev. 2016, 30(2), 74-90.
- 16. Díaz, G.; Córcoles, A.I.; Asencio, A.D.; Torres, M.P. Forest Pathol. 2013, 43(1), 51-58.
- 17. Steindorff, A.S.; Ramada, M.H.S.; Coelho, A.SG.; Miller, RN.G.; Pappas, G.J.; Ulhoa, C.J.; Noronha, E.F. BMC Genomics. 2014, 15(1), 1-14.
- 18. Matarese, F.; Sarrocco, S., Gruber, S.; Seidl-Seiboth, V.; Vannacci, G. Microbiology. 2012, 158, 98-106.
- 19. Rahman, M.A.; Begum, M.F.; Alam, M.F. Mycobiology. 2009, 37(4), 277-285.
- 20. Gao, Z.; Zhang, B.; Liu, H.; Han, J.; Zhang, Y. Biological Control, 2017, 105, 27-39.
- 21. Dennis, C.; Webster, J. Trans Br Mycol Soc. 1971, 57, 41-48.
- 22. Singh, B.N.; Singh, A.; Singh, B.R.; Singh, H.B. J Appl Microbiol. 2013, 116, 654-666.
23. Redda, E.T.; Ma, J.; Mei, J.; Li, M.; Wu, B.; Jiang, X. Eur J Exp Biol. 2018, 8(2), 1-8.
- 24. Datta, P.; Dasgupta, B.; Sengupta, D.K. J Crop Weed. 2011, 7, 202-209.
- 25. Srivastava, R.K.; Singh, R.K.; Kumar, N.; Singh, S. J Biol Contr. 2010, 24, 77-79.
- 26. Ann, J.; Cynthia, C.; Federico, G.; Lani, L. Int J Agric Technol. 2017, 13(7.3), 2539-2548.
- 27. Dal Bello, G.M.; Mónaco, C.I.; Simón, M.R. World J Microb Biot. 2002, 18, 627-636.
- 28. Dendouga, W.; Boureghda, H.; Belhamra. M. Acta Phytopathol Hun. 2016, 51(1), 1-12.
- 29. Stoppacher, N., Kluger, B.; Zeilinger, S.; Krska, R.; Schuhmacher, R. J Microbiol Methods. 2010, 81(2), 187-193.
- 30. Bouanaka, H.; Bellil, I.; Harrat, W.; Boussaha, S.; Benbelkacem, A.; Khelifi, D. Egypt J Biol Pest Co. 2021, 31, 1-13.
- 31. Behzad, H.; Mousa, T.; Mohammad, R.; Mahdi, D. Afr J Biotechnol. 2008, 7(8), 967-972.
- 32. Brotman, Y.; Kapuganti, J.G.; Viterbo, A. Curr Biol. 2010, 20(9), R390-R391.
- 33. Ferrigo, D.; Mondin, M.; Edith, L.; Fabio, F.; Causin, R.; Raiola, A. Biol Control. 2020, 147, 104286.
- 34. Larran, S.; Santamarina Siurana, M.P.; Roselló Caselles, J.; Simón, M.R.; Perelló, A. ACS Omega, 2020, 5(36), 23276-23283.
Year 2024,
Volume: 8 Issue: 1, 69 - 72, 05.06.2024
Aydın Atakan
,
Vakkas Ataş
Project Number
AMYO.ÖKAP.22.01.
References
- 1. Henriksen, B.; Elen, O. J Phytopathol. 2005, 153, 214–220.
- 2. Srivastava, S.K; Huang, X; Brar, H.K.; Fakhoury, A.M.; Bluhm, B.H,; Bhattacharyya, M.K. Plos One. 2014, 9(1), e81832.
- 3. Lanubile, A.; Maschietto, V.; Borrelli, V.M.; Stagnati, L.; Logrieco, A.F.; Marocco, A. Front Plant Sci. 2017, 8, 1774.
- 4. Tian, Y.; Tan, Y.; Yan, Z.; Liao, Y.; Chen, J.; De Boevre, M. Front Microbiol. 2018, 8, 2710.
- 5. Shi, W.; Tan, Y.; Wang, S.; Gardiner, D.M.; De Saeger, S.; Liao, Y.; Wu, A. Toxins. 2016, 9(1), 6.
- 6. Rojas, E.C.; Jørgensen, H.J.; Jensen, B.; Collinge, D.B. Fusarium diseases: biology and management perspectives, 1st ed.; Burleigh Dodds Science Publishing, Cambridge, 2018.
- 7. Wegulo, S.N.; Baenziger, P.S.; Nopsa, J.H.; Bockus, W.W.; Hallen-Adams, H. Crop Prot. 2015, 73, 100-107.
- 8. El-Sobky, M.A.; Fahmi, A.I.; Eissa, R.A.; El-Zanaty, A.M. J Microb Biochem Technol. 2019, 11(1).
- 9. Arora, N.K.; Fatima, T.; Mishra, I.; Verma, S. Microbe-based inoculants: role in next green revolution. In Environmental concerns and sustainable development: Shakla, V., Ed.; Springer, Singapore, 2020; pp. 191-246.
- 10. Troian, R.F.; Steindorff, A.S.; Ramada, M.H.S.; Arruda, W.; Ulhoa, C.J. Biotechnol Lett. 2014, 36, 2095-2101.
11. Martínez-Medina, A.; Del Mar Alguacil, M.; Pascual, J.A.; Van Wees, S., J Chem Ecol. 2014, 40(7), 804-815.
- 12. Li, M.F.; Li, G.H.; Zhang, K.Q. Metabolites. 2019, 9(3), 58.
- 13. Sarrocco, S.; Esteban, P.; Vicente, I.; Bernardi, R.; Plainchamp, T.; Domenichini, S.; Dufresne, M. Phytopathology. 2021, 111(7), 1129-1136.
- 14. Alfiky, A.; Weisskopf, L. J Fungi. 2021, 7(1), 61.
- 15. Zeilinger, S.; Gruber, S.; Bansal, R.; Mukherjee, P.K. Fungal Biol Rev. 2016, 30(2), 74-90.
- 16. Díaz, G.; Córcoles, A.I.; Asencio, A.D.; Torres, M.P. Forest Pathol. 2013, 43(1), 51-58.
- 17. Steindorff, A.S.; Ramada, M.H.S.; Coelho, A.SG.; Miller, RN.G.; Pappas, G.J.; Ulhoa, C.J.; Noronha, E.F. BMC Genomics. 2014, 15(1), 1-14.
- 18. Matarese, F.; Sarrocco, S., Gruber, S.; Seidl-Seiboth, V.; Vannacci, G. Microbiology. 2012, 158, 98-106.
- 19. Rahman, M.A.; Begum, M.F.; Alam, M.F. Mycobiology. 2009, 37(4), 277-285.
- 20. Gao, Z.; Zhang, B.; Liu, H.; Han, J.; Zhang, Y. Biological Control, 2017, 105, 27-39.
- 21. Dennis, C.; Webster, J. Trans Br Mycol Soc. 1971, 57, 41-48.
- 22. Singh, B.N.; Singh, A.; Singh, B.R.; Singh, H.B. J Appl Microbiol. 2013, 116, 654-666.
23. Redda, E.T.; Ma, J.; Mei, J.; Li, M.; Wu, B.; Jiang, X. Eur J Exp Biol. 2018, 8(2), 1-8.
- 24. Datta, P.; Dasgupta, B.; Sengupta, D.K. J Crop Weed. 2011, 7, 202-209.
- 25. Srivastava, R.K.; Singh, R.K.; Kumar, N.; Singh, S. J Biol Contr. 2010, 24, 77-79.
- 26. Ann, J.; Cynthia, C.; Federico, G.; Lani, L. Int J Agric Technol. 2017, 13(7.3), 2539-2548.
- 27. Dal Bello, G.M.; Mónaco, C.I.; Simón, M.R. World J Microb Biot. 2002, 18, 627-636.
- 28. Dendouga, W.; Boureghda, H.; Belhamra. M. Acta Phytopathol Hun. 2016, 51(1), 1-12.
- 29. Stoppacher, N., Kluger, B.; Zeilinger, S.; Krska, R.; Schuhmacher, R. J Microbiol Methods. 2010, 81(2), 187-193.
- 30. Bouanaka, H.; Bellil, I.; Harrat, W.; Boussaha, S.; Benbelkacem, A.; Khelifi, D. Egypt J Biol Pest Co. 2021, 31, 1-13.
- 31. Behzad, H.; Mousa, T.; Mohammad, R.; Mahdi, D. Afr J Biotechnol. 2008, 7(8), 967-972.
- 32. Brotman, Y.; Kapuganti, J.G.; Viterbo, A. Curr Biol. 2010, 20(9), R390-R391.
- 33. Ferrigo, D.; Mondin, M.; Edith, L.; Fabio, F.; Causin, R.; Raiola, A. Biol Control. 2020, 147, 104286.
- 34. Larran, S.; Santamarina Siurana, M.P.; Roselló Caselles, J.; Simón, M.R.; Perelló, A. ACS Omega, 2020, 5(36), 23276-23283.