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Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant

Year 2024, Volume: 8 Issue: 1, 73 - 82, 05.06.2024

Osman Bedrettin Akkaya İbrahim Seyfettin Çelik Erdal Ertaş Nazan Çömlekcioğlu Ashabil Aygan

https://doi.org/10.32571/ijct.1445857

Abstract

Hypericum perforatum L. (St John's wort), although primarily utilized in traditional medicine, is also frequently employed in modern therapy. Within the scope of the study, H. perforatum was collected from Kahramanmaraş region and dried. Extracts derived from above-ground parts were analyzed to determine their levels of total phenolic and flavonoid compounds, as well as their antioxidant, anticancer and antimicrobial properties. Following the GC-MS analysis of the extracts, a total of 18 distinct fatty acids were identified. The main fatty acid components were identified as behenic (37.90 %), linoleic (21.22 %), gamma-linolenic (15.87 %), oleic (9.45 %) and palmitic acid (7.64 %). The plant extracts were found to have a total phenolic content of 60.22 mg GAE g-1 and a flavonoid content of 7.68 µg QE g-1, as determined through analysis. Additionally, FRAP and IC50 values were determined as 26.96 µg AAE g-1 and 0.44 µg mL-1, respectively. The antimicrobial activities of H. perforatum extracts were investigated among a total of 11 microorganisms, including 9 bacteria and 2 yeasts. It has been observed that extracts possess significant antimicrobial activity against all tested microorganisms. H. perforatum extracts were observed to have dose-dependent inhibition of all organisms. It was observed that H. perforatum extracts killed cancer cells at concentrations of 0.1 mg mL-1 and above on H1299, MCF-7 and HUVEC cancer cell lines.

Keywords

1st H1299 2nd bioactive compounds 3rd anticarcinogenic activity 4th hypericum perforatum 5th fatty acids

References

  • 1. Galeotti, N. J. Ethnopharmacology. 2017, 200, 136-146.
  • 2. Kapoor, S.; Chandel, R.; Kaur, R.; Kumar, S., Kumar, R.; Janghu, S.; ... & Kumar, V. Biochem. Syst. Ecol.. 2023, 110, 104702.
  • 3. Seyis, F.; Yurteri, E.; Ozcan, A.; Cirak, C. S. Afr. J. Bot.. 2020, 135, 391-403.
  • 4. Baytop T. Nobel Medical Bookstores. 1999, İstanbul, 166-167.
  • 5. El-Chaghaby, G. A.; Mohammed, F. S.; Rashad, S.; Uysal, I.; Kocer, O.; Lekesiz, O., Sevindik, M. Egypt. J. Bot. 2024, 64(1), 1-26.
  • 6. Nobakht, S. Z.; Akaberi, M.; Mohammadpour, A. H.; Moghadam, A. T.; Emami, S. A. Iran. J. Basic Med. Sci.. 2022, 25(9), 1045.
  • 7. Kwiecień, I.; Miceli, N.; Kędzia, E.; Cavò, E.; Taviano, M. F.; Beerhues, L.; Ekiert, H. Molecules. 2023, 28(5), 2376.
  • 8. Bayram, E.; Geren, H.; Avci, A. B.; Arabaci, O. J. Ege Univ. Fac. Agric. 2004, 41(2).
  • 9. Ekren, S. J. Agric. Sci.. 2010, 16(4).
  • 10. Filippini, R.; Piovan, A.; Borsarini, A.; Caniato, R. Fitoterapia 2010, 81, 115–119.
  • 11. Sahebkar-Khorasani, M.; Safarian, M.; Jarahi, L.; Yousefi, M.; Salari, R.; Meshkat, M.; Ayati, M.H.; Bahrami-Taghanaki, H.; Kargozar, R.; Azizi, H. Eur. J. Integr. Med. 2022, 52, 102119.
  • 12. Kholghi, G.; Arjmandi-Rad, S.; Zarrindast, M.-R.; Vaseghi, S. Naunyn Schmiedebergs Arch. Pharmacol. 2022, 395, 629–642.
  • 13. Nahrstedt, A.; Butterweck, V. J. Nat. Prod. 2010, 73, 1015–1021.
  • 14. Linde, K. St. John’s Wort-An overview. Forsch. Komplementarmed. 2009, 16, 146–155.
  • 15. Davis, P.H. Flora of Turkey and the East Aegean Islands, Edinburgh University. 1965, Press.
  • 16. Comlekcioglu, N. Braz. Arch. Biol. Technol.. 2019, 62, e19180330.
  • 17. Blainski, A.; Lopes, G.C.; De Mello, J.C.P. Molecules. 2013, 18, 6852-6865. https://doi.org/10.3390/molecules18066852.
  • 18. Chang, C.C.; Yang, M. H.; Wen, H. M.; Chern, J. C. J Food Drug Anal. 2002, 10 (3): 178-182.
  • 19. Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Food Sci. Technol.. 1995, Volume 28, Issue 1, 1995, Pages 25-30.
  • 20. Benzie, I. F.; Strain, J. J. AnalBiochem. 1996, 239 (1): 70-76.
  • 21. NCCLS (National Committee for Clinical Laboratory Standards), 1993.Dilution, Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standards. NCCLS Document. M7-A3.
  • 22. Collins, C. H.; Lyne, P. M.; Grange, J. M. 1989. Collins and Lyne’s Microbiological Methods, Sixth Edition, Butterworths Co. Ltd. London.
  • 23. Pelliccia, F.; Marazzi, G.; Greco, C.; Franzoni, F.; Speziale, G.; Gaudio, C. Int. J. Cardiol. 2013, 170: S3- S7.
  • 24. Jabeur, I.; Pereira, E.; Barros, L.; Calhelha, R. C.; Soković, M.; Oliveira, M. B. P.; Ferreira, I. C. Food Res. Int. 2017, 100: 717-723.
  • 25. Hosni, K.; Msaâda, K.; Taârit, M. B.; Marzouk, B. Arab. J. Chem. 2013, 10, S2736–S2741.
  • 26. Bakir, T. K. Turk. J. Agric. Food Sci. Technol. 2018, 6(11): 1647-1652.
  • 27. Stojanovic, G.; Palic, R.; Tarr, C.H.; Reddy, C.M.; Marinkovic, O. Biochem. Syst. Ecol. 2003, 31, 223–226.
  • 28. Sevindik M. Mycopath. 2018, 16(2): 47-49.
  • 29. Mohammed, F. S.; Sabik, A. E.; Dogan, M.; Selamoglu, Z., Sevindik, M. Bull. Biotechnol. 2020, 1(2), 43-45.
  • 30. Raziq, N.; Muhammad, N.; Chishti, K. A.; Saeed, M.; Rahman, S.; Khan, H. Afr. J. Pharm. Pharmacol. 2011, Vol. 5(16), pp. 1872-1876.
  • 31. Piatti, D.; Marconi R.; Caprioli, G.; Angeloni, S.; Ricciutelli, M.; Zengin, G.; Maggi, F.; Pagni, L.; Sagratini, G. L. Appl. Sci. 2023, 13, 13304.
  • 32. Mahomoodally, M.F.; Zengin, G.; Roumita, S.; Caprioli, G.; Mustafa, A.M.; Piatti, D.; Yıldıztugay, E.; Ak, G.; Karada˘g, A.E.; Khalid, A.; et al. Chem. Biodivers. 2023, 20, e202201181.
  • 33. Napoli, E.; Siracusa, L.; Ruberto, G.; Carrubba, A.; Lazzara, S.; Speciale, A.; Cimino, F.; Saija, A.; Cristani, M. Phytochemistry. 2018, 152, 162–173.
  • 34. Alahmad, A.; Alghoraibi, I.; Zein, R.; Kraft, S.; Dräger, G.; Walter, J.G.; Scheper, T. ACS Omega. 2022, 7, 13475–13493.
  • 35. Ozturk, N.; Tuncel, M.; Poto˘glu-Erkara, ˙I. Pharm. Biol. 2009, 47, 120–127.
  • 36. Lourenco, S.C.; Moldão-Martins, M.; Alves, V.D. Molecules. 2019, 24, 4132.
  • 37. Conforti, F.; Statti, G. A., Tundis, B.; Bianchi, A.; Agrimonti, C.; Sacchetti, G.; Andreotti, E.; Menichini, F.; Poli, F. Nat. Prod. Res.. 2005, 19(3), 295,303.
  • 38. Agnol, R. D.; Ferraz, A.; Bernardi, A. P.; Albring, D.; Nor, Sarmento, C. L.; Lamb, L., Hass, M.; Poser, G.; Schapoval, E. S. Phytomedicine. 2003, 10, 511–516.
  • 39. Torre, L. A.; Siegel, R. L.; Ward, E. M.; Jemal, A. Cancer Epidemiol Biomarkers Prev. 2016, 25 (1): 16–27. https://doi.org/10.1158/1055-9965.EPI-15-0578.
  • 40. Karakas, N.; Okur, M. E.; Ozturk, I.; Ayla, S.; Karadag, A. E., Polat, D. C. Medeni Med J. 2019, 34(3):297-304.
  • 41. Nair, H. K.; Rao, K.V.; Aalinkeel, R.; Mahajan, S., Chawda, R.; Schwartz, S. A. Clin. Diagn. Lab Immunol. 2004, 11 63–69.
  • 42. Matic, I. Z.; Ergun S.; Crnogorac M. Đ.; Misir S., Aliyazicioglu Y., Damjanović A.; Džudžević-Čančar H., Stanojković T.; Konanc K., Petrović N. Cytotechnology. 2021, 73: 373–389.

Year 2024, Volume: 8 Issue: 1, 73 - 82, 05.06.2024

Osman Bedrettin Akkaya İbrahim Seyfettin Çelik Erdal Ertaş Nazan Çömlekcioğlu Ashabil Aygan

https://doi.org/10.32571/ijct.1445857

Abstract

References

  • 1. Galeotti, N. J. Ethnopharmacology. 2017, 200, 136-146.
  • 2. Kapoor, S.; Chandel, R.; Kaur, R.; Kumar, S., Kumar, R.; Janghu, S.; ... & Kumar, V. Biochem. Syst. Ecol.. 2023, 110, 104702.
  • 3. Seyis, F.; Yurteri, E.; Ozcan, A.; Cirak, C. S. Afr. J. Bot.. 2020, 135, 391-403.
  • 4. Baytop T. Nobel Medical Bookstores. 1999, İstanbul, 166-167.
  • 5. El-Chaghaby, G. A.; Mohammed, F. S.; Rashad, S.; Uysal, I.; Kocer, O.; Lekesiz, O., Sevindik, M. Egypt. J. Bot. 2024, 64(1), 1-26.
  • 6. Nobakht, S. Z.; Akaberi, M.; Mohammadpour, A. H.; Moghadam, A. T.; Emami, S. A. Iran. J. Basic Med. Sci.. 2022, 25(9), 1045.
  • 7. Kwiecień, I.; Miceli, N.; Kędzia, E.; Cavò, E.; Taviano, M. F.; Beerhues, L.; Ekiert, H. Molecules. 2023, 28(5), 2376.
  • 8. Bayram, E.; Geren, H.; Avci, A. B.; Arabaci, O. J. Ege Univ. Fac. Agric. 2004, 41(2).
  • 9. Ekren, S. J. Agric. Sci.. 2010, 16(4).
  • 10. Filippini, R.; Piovan, A.; Borsarini, A.; Caniato, R. Fitoterapia 2010, 81, 115–119.
  • 11. Sahebkar-Khorasani, M.; Safarian, M.; Jarahi, L.; Yousefi, M.; Salari, R.; Meshkat, M.; Ayati, M.H.; Bahrami-Taghanaki, H.; Kargozar, R.; Azizi, H. Eur. J. Integr. Med. 2022, 52, 102119.
  • 12. Kholghi, G.; Arjmandi-Rad, S.; Zarrindast, M.-R.; Vaseghi, S. Naunyn Schmiedebergs Arch. Pharmacol. 2022, 395, 629–642.
  • 13. Nahrstedt, A.; Butterweck, V. J. Nat. Prod. 2010, 73, 1015–1021.
  • 14. Linde, K. St. John’s Wort-An overview. Forsch. Komplementarmed. 2009, 16, 146–155.
  • 15. Davis, P.H. Flora of Turkey and the East Aegean Islands, Edinburgh University. 1965, Press.
  • 16. Comlekcioglu, N. Braz. Arch. Biol. Technol.. 2019, 62, e19180330.
  • 17. Blainski, A.; Lopes, G.C.; De Mello, J.C.P. Molecules. 2013, 18, 6852-6865. https://doi.org/10.3390/molecules18066852.
  • 18. Chang, C.C.; Yang, M. H.; Wen, H. M.; Chern, J. C. J Food Drug Anal. 2002, 10 (3): 178-182.
  • 19. Brand-Williams, W.; Cuvelier, M.E.; Berset, C. Food Sci. Technol.. 1995, Volume 28, Issue 1, 1995, Pages 25-30.
  • 20. Benzie, I. F.; Strain, J. J. AnalBiochem. 1996, 239 (1): 70-76.
  • 21. NCCLS (National Committee for Clinical Laboratory Standards), 1993.Dilution, Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically. Approved Standards. NCCLS Document. M7-A3.
  • 22. Collins, C. H.; Lyne, P. M.; Grange, J. M. 1989. Collins and Lyne’s Microbiological Methods, Sixth Edition, Butterworths Co. Ltd. London.
  • 23. Pelliccia, F.; Marazzi, G.; Greco, C.; Franzoni, F.; Speziale, G.; Gaudio, C. Int. J. Cardiol. 2013, 170: S3- S7.
  • 24. Jabeur, I.; Pereira, E.; Barros, L.; Calhelha, R. C.; Soković, M.; Oliveira, M. B. P.; Ferreira, I. C. Food Res. Int. 2017, 100: 717-723.
  • 25. Hosni, K.; Msaâda, K.; Taârit, M. B.; Marzouk, B. Arab. J. Chem. 2013, 10, S2736–S2741.
  • 26. Bakir, T. K. Turk. J. Agric. Food Sci. Technol. 2018, 6(11): 1647-1652.
  • 27. Stojanovic, G.; Palic, R.; Tarr, C.H.; Reddy, C.M.; Marinkovic, O. Biochem. Syst. Ecol. 2003, 31, 223–226.
  • 28. Sevindik M. Mycopath. 2018, 16(2): 47-49.
  • 29. Mohammed, F. S.; Sabik, A. E.; Dogan, M.; Selamoglu, Z., Sevindik, M. Bull. Biotechnol. 2020, 1(2), 43-45.
  • 30. Raziq, N.; Muhammad, N.; Chishti, K. A.; Saeed, M.; Rahman, S.; Khan, H. Afr. J. Pharm. Pharmacol. 2011, Vol. 5(16), pp. 1872-1876.
  • 31. Piatti, D.; Marconi R.; Caprioli, G.; Angeloni, S.; Ricciutelli, M.; Zengin, G.; Maggi, F.; Pagni, L.; Sagratini, G. L. Appl. Sci. 2023, 13, 13304.
  • 32. Mahomoodally, M.F.; Zengin, G.; Roumita, S.; Caprioli, G.; Mustafa, A.M.; Piatti, D.; Yıldıztugay, E.; Ak, G.; Karada˘g, A.E.; Khalid, A.; et al. Chem. Biodivers. 2023, 20, e202201181.
  • 33. Napoli, E.; Siracusa, L.; Ruberto, G.; Carrubba, A.; Lazzara, S.; Speciale, A.; Cimino, F.; Saija, A.; Cristani, M. Phytochemistry. 2018, 152, 162–173.
  • 34. Alahmad, A.; Alghoraibi, I.; Zein, R.; Kraft, S.; Dräger, G.; Walter, J.G.; Scheper, T. ACS Omega. 2022, 7, 13475–13493.
  • 35. Ozturk, N.; Tuncel, M.; Poto˘glu-Erkara, ˙I. Pharm. Biol. 2009, 47, 120–127.
  • 36. Lourenco, S.C.; Moldão-Martins, M.; Alves, V.D. Molecules. 2019, 24, 4132.
  • 37. Conforti, F.; Statti, G. A., Tundis, B.; Bianchi, A.; Agrimonti, C.; Sacchetti, G.; Andreotti, E.; Menichini, F.; Poli, F. Nat. Prod. Res.. 2005, 19(3), 295,303.
  • 38. Agnol, R. D.; Ferraz, A.; Bernardi, A. P.; Albring, D.; Nor, Sarmento, C. L.; Lamb, L., Hass, M.; Poser, G.; Schapoval, E. S. Phytomedicine. 2003, 10, 511–516.
  • 39. Torre, L. A.; Siegel, R. L.; Ward, E. M.; Jemal, A. Cancer Epidemiol Biomarkers Prev. 2016, 25 (1): 16–27. https://doi.org/10.1158/1055-9965.EPI-15-0578.
  • 40. Karakas, N.; Okur, M. E.; Ozturk, I.; Ayla, S.; Karadag, A. E., Polat, D. C. Medeni Med J. 2019, 34(3):297-304.
  • 41. Nair, H. K.; Rao, K.V.; Aalinkeel, R.; Mahajan, S., Chawda, R.; Schwartz, S. A. Clin. Diagn. Lab Immunol. 2004, 11 63–69.
  • 42. Matic, I. Z.; Ergun S.; Crnogorac M. Đ.; Misir S., Aliyazicioglu Y., Damjanović A.; Džudžević-Čančar H., Stanojković T.; Konanc K., Petrović N. Cytotechnology. 2021, 73: 373–389.
There are 42 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Research Articles
Authors

Osman Bedrettin Akkaya 0000-0002-8606-881X

İbrahim Seyfettin Çelik 0000-0001-6946-4477

Erdal Ertaş 0000-0002-4043-5960

Nazan Çömlekcioğlu 0000-0001-7729-5271

Ashabil Aygan 0000-0003-4936-9872

Early Pub Date May 30, 2024
Publication Date June 5, 2024
Submission Date March 1, 2024
Acceptance Date May 30, 2024
Published in Issue Year 2024 Volume: 8 Issue: 1

Cite

APA Akkaya, O. B., Çelik, İ. S., Ertaş, E., Çömlekcioğlu, N., et al. (2024). Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. International Journal of Chemistry and Technology, 8(1), 73-82. https://doi.org/10.32571/ijct.1445857
AMA Akkaya OB, Çelik İS, Ertaş E, Çömlekcioğlu N, Aygan A. Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. Int. J. Chem. Technol. June 2024;8(1):73-82. doi:10.32571/ijct.1445857
Chicago Akkaya, Osman Bedrettin, İbrahim Seyfettin Çelik, Erdal Ertaş, Nazan Çömlekcioğlu, and Ashabil Aygan. “Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant”. International Journal of Chemistry and Technology 8, no. 1 (June 2024): 73-82. https://doi.org/10.32571/ijct.1445857.
EndNote Akkaya OB, Çelik İS, Ertaş E, Çömlekcioğlu N, Aygan A (June 1, 2024) Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. International Journal of Chemistry and Technology 8 1 73–82.
IEEE O. B. Akkaya, İ. S. Çelik, E. Ertaş, N. Çömlekcioğlu, and A. Aygan, “Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant”, Int. J. Chem. Technol., vol. 8, no. 1, pp. 73–82, 2024, doi: 10.32571/ijct.1445857.
ISNAD Akkaya, Osman Bedrettin et al. “Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant”. International Journal of Chemistry and Technology 8/1 (June 2024), 73-82. https://doi.org/10.32571/ijct.1445857.
JAMA Akkaya OB, Çelik İS, Ertaş E, Çömlekcioğlu N, Aygan A. Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. Int. J. Chem. Technol. 2024;8:73–82.
MLA Akkaya, Osman Bedrettin et al. “Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant”. International Journal of Chemistry and Technology, vol. 8, no. 1, 2024, pp. 73-82, doi:10.32571/ijct.1445857.
Vancouver Akkaya OB, Çelik İS, Ertaş E, Çömlekcioğlu N, Aygan A. Determination of Antimicrobial, Anticarcinogenic Activity of Bioactive Components of Hypericum Perforatum L. Plant. Int. J. Chem. Technol. 2024;8(1):73-82.
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