Molecular biology studies in plants require high quality DNA isolation from the taxa studied. Therefore, DNA isolation with high concentration and as pure as possible from plants to be used in studies is extremely important. There are different protocols for genomic DNA isolation from plants. In this study, some taxa belonging to Asteraceae, Apiaceae and Lamiaceae families of medicinal and aromatic plants were used. It has been tried to investigate which types of tissues are more effective by taking samples from both dry leaf tissues of taxa that have been turned into herbarium material and fresh leaf tissues. In the methods used, only CTAB and CTAB + PVP were prepared together in extraction buffer solutions, in addition to this, a ready-made isolation kit was used. In general, high concentrations of DNA were obtained from all methods, while the amount of proteins, RNA, polysaccharides, essential oils, phenols and other contaminants in the gDNA obtained by these methods were tried to be minimized. While the concentration and purity of the isolated DNA was measured in a nanodrop spectrophotometer, its density was visualized in agarose gel electrophoresis. In addition, the suitability of the obtained DNAs for PCR studies was tested using various primers. Although the amount and quality of the obtained DNA varies between taxa, the best quality isolation has always been obtained from isolations using fresh plant material. However, the extraction method using only CTAB solution provided DNA with the highest purity and density. Following this, it was observed that CTAB + PVP methods gave similar results, while the use of ready-made kits was not very useful in DNA isolation from plants. Although the use of ready-made kits gives clearer looking DNA, the obtained DNA was found to be insufficient and did not give positive results in PCR studies.