Year 2020, Volume 7 , Issue 3, Pages 61 - 68 2020-10-05

Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001

Gökhan KARS [1] , Ümmühan ALPARSLAN [2]


The objective of this work was to reveal optimum culture composition for hydrogen and 5-aminolevulinic acid productions by Rhodobacter sphaeroides O.U.001 regarding substrate concentration and supplementations of elements and vitamins. Acetate was chosen as carbon source and five distinct concentrations (20, 25, 30, 35 and 40 mM) were tested in two experimental setups. While, elements (FeSO4, 2 g L-1 and Na2MoO4.2H2O, 0.2 g L-1) and vitamins (Biotin, 0.015 g L-1, Niacin, 0.5 g L-1 and Thiamine, 0.5 g L-1) were added into the media in the first setup, they were omitted in the latter for comparison. As a result, the highest hydrogen production (0.33 L H2 L-1 culture) was attained in the presence of supplements using 20 mM acetate. Similarly, the maximum amount of 5-ALA generation (16.54 mM) was achieved in 20 mM acetate containing medium under the same conditions. On the other hand, the greatest bacterial growth (OD660: 4.412, 2.162 g cdw L-1) was achieved in the absence of supplements using 40 mM acetate. To conclude, while element and vitamin supplementations promoted hydrogen and 5-ALA productions, absence of these had a positive effect on cell biomass. Specifically, the medium containing 20 mM acetate together with elements and vitamins could be suggested as the optimum growth culture for the highest hydrogen and 5-ALA productions.
Acetate, aminolevulinic acid, hydrogen, Rhodobacter sphaeroides
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Primary Language en
Subjects Basic Sciences
Journal Section Research Article
Authors

Orcid: 0000-0002-2507-2305
Author: Gökhan KARS (Primary Author)
Institution: NECMETTİN ERBAKAN ÜNİVERSİTESİ, FEN FAKÜLTESİ, MOLEKÜLER BİYOLOJİ VE GENETİK BÖLÜMÜ
Country: Turkey


Orcid: 0000-0002-4107-3420
Author: Ümmühan ALPARSLAN
Institution: SELCUK UNIVERSITY, FACULTY OF SCIENCE, DEPARTMENT OF BIOLOGY
Country: Turkey


Supporting Institution Selçuk Üniversitesi
Project Number BAP-11401112
Thanks This study was supported by Selçuk University with the project number BAP-11401112.
Dates

Application Date : May 16, 2020
Acceptance Date : July 8, 2020
Publication Date : October 5, 2020

Bibtex @research article { ijeat738318, journal = {International Journal of Energy Applications and Technologies}, issn = {}, eissn = {2548-060X}, address = {editor.ijeat@gmail.com}, publisher = {İlker ÖRS}, year = {2020}, volume = {7}, pages = {61 - 68}, doi = {10.31593/ijeat.738318}, title = {Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001}, key = {cite}, author = {Kars, Gökhan and Alparslan, Ümmühan} }
APA Kars, G , Alparslan, Ü . (2020). Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001 . International Journal of Energy Applications and Technologies , 7 (3) , 61-68 . DOI: 10.31593/ijeat.738318
MLA Kars, G , Alparslan, Ü . "Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001" . International Journal of Energy Applications and Technologies 7 (2020 ): 61-68 <https://dergipark.org.tr/en/pub/ijeat/issue/57106/738318>
Chicago Kars, G , Alparslan, Ü . "Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001". International Journal of Energy Applications and Technologies 7 (2020 ): 61-68
RIS TY - JOUR T1 - Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001 AU - Gökhan Kars , Ümmühan Alparslan Y1 - 2020 PY - 2020 N1 - doi: 10.31593/ijeat.738318 DO - 10.31593/ijeat.738318 T2 - International Journal of Energy Applications and Technologies JF - Journal JO - JOR SP - 61 EP - 68 VL - 7 IS - 3 SN - -2548-060X M3 - doi: 10.31593/ijeat.738318 UR - https://doi.org/10.31593/ijeat.738318 Y2 - 2020 ER -
EndNote %0 International Journal of Energy Applications and Technologies Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001 %A Gökhan Kars , Ümmühan Alparslan %T Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001 %D 2020 %J International Journal of Energy Applications and Technologies %P -2548-060X %V 7 %N 3 %R doi: 10.31593/ijeat.738318 %U 10.31593/ijeat.738318
ISNAD Kars, Gökhan , Alparslan, Ümmühan . "Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001". International Journal of Energy Applications and Technologies 7 / 3 (October 2020): 61-68 . https://doi.org/10.31593/ijeat.738318
AMA Kars G , Alparslan Ü . Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001. IJEAT. 2020; 7(3): 61-68.
Vancouver Kars G , Alparslan Ü . Unraveling optimum culture composition for hydrogen and 5-aminolevulinic acid production by Rhodobacter sphaeroides O.U.001. International Journal of Energy Applications and Technologies. 2020; 7(3): 61-68.