Total Phenolic Content and Radical Scavenging Activity of Carthamus Tinctorius L.

Abstract

Extract from leaves of Carthamus tinctorius L. was prepared by using soxhlet extraction method. Total phenolic, flavonoid content and also antioxidant activity of leaves from Carthamus tinctorius L. were determined. To determine the antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH•) scavenging, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+) radical scavenging tests were used. Reducing power of the extract was also tested. Total phenolic and flavonoid content of the extract were determined as 77.38 ± 6.62 mg GAE/g, 25.49 ± 0.26 mg CE/g dry weight of extract respectively. DPPH radical scavenging activity was determined as 89%. ABTS radical scavenging activity was found as 58% at 1 mg/mL concentration. Reducing power was found as 0.885.

Keywords

• safflower, antioxidant activity, phenolic, Carthamus tinctorius L.

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