Analysis of Anti-cancer Potential of Solanum Virginianum L. extract of fruit and leaf by using In Vitro and In Silico Studies

Year 2025, Volume: 8 Issue: 2, 89 - 98, 25.12.2025

Madhu Kushwaha , Dr Pankaj Tiwari

Abstract

Solanum virginianum L. has been recognized as a traditional medicinal plant within Ayurveda. Aim: This study aimed to investigate the anticancer properties of S. virginianum through both in vitro and in silico methodologies. Methods: The fruit and leaves of the plant underwent hot extraction utilizing methanol and water as solvents. A cytotoxicity assay was conducted on the MCF-7 (Adenocarcinoma breast cancer cell line) and HEK-293 (Human embryonic kidney cell line—a normal cell line). Additionally, cell migration inhibition assays and colony formation assays were performed specifically on the MCF-7 cell line. The leaf aqueous extract was analyzed using HR-LCMS/MS to identify various phytocompounds. The in-silico analysis involved assessing the binding affinity between nine selected phytocompounds and specific apoptotic target proteins. Results: Among all tested extracts, the leaf aqueous extracts exhibited notable efficacy, with an IC50 concentration of 10.4 ± 1.13 µg/mL at the 72-hour incubation period. The leaf aqueous extract also demonstrated significant performance in the cell migration inhibition and colony-forming assays compared to the fruit extracts. Metabolite identification in the aqueous leaf extract through HR-LCMS/MS revealed the presence of over 30 phytocompounds. Nine chosen phytocompounds were characterized for their capacity to bind to specific apoptotic target proteins. Of the docked compounds, ritterazine A exhibited exceptionally strong binding, with a binding energy exceeding -9 kcal/mol across all target proteins. Conclusion: This study concludes that the leaf of S. virginianum possesses potent anti-cancer properties. Moreover, there exists a promising avenue for extensive research into the therapeutic applications of this plant in the treatment of breast cancer.

Keywords

anti-cancer , medicinal plant , invitro vivo , cytotoxicity

References

• Danaei G, Vander Hoorn S, Lopez AD, Murray CJ, Ezzati M. Causes of cancer in the world: comparative risk assessment of nine behavioural and environmental risk factors. Lancet. 2005; 366(9499):1784-93. https://doi.org/10.1016/S0140-6736(05)67725-2 PMid:16298215
• Jia LT, Zhang R, Shen L, Yang AG. Regulators of carcinogenesis: emerging roles beyond their primary functions. Cancer Lett. 2015; 357(1):75-82. https://doi. org/10.1016/j.canlet.2014.11.048 PMid:25448403
• Singh P, Ngcoya N, Kumar V. A review of the recent developments in synthetic anti-breast cancer agents. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents). 2016; 16(6):668-85. https://doi.org/10.2174/1871520616666151120122120 PMid:26584726
• Rajeswara Rao BR. Genetic diversity, genetic erosion, conservation of genetic resources, and cultivation of medicinal plants. Genetic Diversity and Erosion in Plants: Case Histories. 2015. p. 357-407. https://doi. org/10.1007/978-3-319-25954-3_11
• Kumar S, Pandey AK. Medicinal attributes of Solanum xanthocarpum fruit consumed by several tribal communities as food: an in vitro antioxidant, anticancer and anti-HIV perspective. BMC Complement Altern Med. 2014; 14:1-8. https://doi.org/10.1186/1472-6882-14-112 PMid:24678980 PMCid: PMC3973604
• Chaturvedi PK, Bhui K, Shukla Y. Lupeol: connotations for chemoprevention. Cancer Lett. 2008; 263(1):1-3. https:// doi.org/10.1016/j.canlet.2008.01.047 PMid:18359153
• Kuo KW, Hsu SH, Li YP, Lin WL, Liu LF, Chang LC, Lin CC, Lin CN, Sheu HM. Anticancer activity evaluation of the Solanum glycol alkaloids olamargine: triggering apoptosis in human hepatoma cells. Biochem Pharmacol. 2000; 60(12):1865-73. https://doi.org/10.1016/S0006-2952(00)00506-2 PMid:11108802
• Siddique YH, Beg T, Afzal M. Antigenotoxic effect of apigenin against anti-cancerous drugs. Toxicol In Vitro. 2008; 22(3):625-31. https://doi.org/10.1016/j. tiv.2007.12.002 PMid:18206345
• Rao TB, Sreedhar G. In vitro cytotoxicity activity of Solanum xanthocarpum against MCF-7, HeLa, A549 and CaCo2 cell lines by MTT assay. Eur J Mol Clin Med. 2021; 8(2):638-43
• Saraswathi K, Bharkavi R, Khusro A, Sivaraj C, Arumugam P, Alghamdi S, Dablool AS, Almehmadi M, Bannunah AM, Sahibzada MU. Assessment of in vitro medicinal properties and chemical composition analysis of Solanum virginianum dried fruits. Arab J Chem. 2021; 14(12):103442. https://doi. org/10.1016/j.arabjc.2021.103442
• Srimathi R, Raja MK, Kathiravan MK. In silico screening of traditional herbal medicine-derived chemical constituents for possible potential inhibition against sars-cov-2. J Nat Remedies. 2020:79-88. https://doi.org/10.18311/ jnr/2020/25278
• Chitre TS, Panda S, Patil SM, Chothe AS, Vignesh G, Kathiravan MK. Novel 1, 3, 4-(thiadiazol-2-ylamino) methyl-5-(pyridin-4-yl)-1, 3, 4-oxadiazol-2-thiones: synthesis, docking and antimycobacterial testing. Adv Biol Chem. 2011; 1(02):7. https://doi.org/10.4236/ abc.2011.12002