Evaluation of Serum 25-(OH)D Vitamin Levels in Healthy and Pregnancy Toxemic Goats

Year 2025, Volume: 5 Issue: 2, 83 - 91, 30.09.2025

Murat Uztimür , Nebi Akbulut , Hakan Keçeci , Cennet Nur Ünal

Abstract

The goal of the research was to assess serum 25-(OH) D concentration in healthy goats and goats with pregnancy toxemia.This research used 30 goats during the final three weeks of gestation. 10 of them were evaluated as a control group (CG), 10 as subclinical pregnancy toxemia (SPT), and the remaining 10 as clinical pregnancy toxemia (CPT). Pregnancy toxemia in goats was diagnosed by analysis of blood beta-hydroxybutyric acid (BHBA) concentration in the Freestyle Optium Neo-H device. While glucose, iron, and unsaturated iron-binding capacity (UIBC) concentrations were analyzed by an automatic biochemistry analyzer, serum 25-(OH) D concentration was investigation by liquid chromatography-mass spectrometry. Blood BHBA concentrations in SPT and CPT were statistically elevated versus the control group (P<0.001). The 25-(OH) D vitamin level in goats with pregnant toxemia had been found to be substantially lower than that in the control group (P<0.001). The serum iron concentration in goats with pregnant toxemia was substantially lower than that in the CG (P<0.010). The sensitivity of serum 25-(OH) D in the diagnosis of pregnancy toxemia was 90%, specificity was 95%, the threshold value was 26.12 µg/L, and the AUC value was 0.95. It was observed that pregnancy toxemia in goats showed a strong negative correlation with 25-(OH) D and BHBA and a significant positive correlation with glucose and iron. This study concluded that the 25-(OH) D levels in goats with pregnant toxemia were dramatically reduced and exhibited a notable correlation with inflammation.

Keywords

goat , 25-OH , BHBA , Iron

Project Number

2024/1-1919B012323573

Sağlıklı ve Gebelik Toksemili Keçilerde Serum 25-(OH)D Konsantrasyonunun Değerlendirilmesi

Abstract

Çalışmanın amacı sağlıklı keçilerde ve gebelik toksemisine sahip keçilerde serum 25-(OH) konsantrasyonunu değerlendirmektir. Bu araştırmada gebeliklerinin son üç haftasında olan 30 keçi kullanıldı. Bunlardan 10 tanesi kontrol grubu (CG), 10 tanesi subklinik gebelik toksemi (SPT) ve kalan 10 tanesi ise klinik gebelik toksemi (CPT) olarak değerlendirildi. Keçilerde gebelik toksemi tanısı, Freestyle Optium Neo-H cihazında kan beta-hidroksibütirik asit (BHBA) konsantrasyonunun analizi ile konuldu. Glikoz, demir ve doymamış demir bağlama kapasitesi (UIBC) konsantrasyonları otomatik biyokimya analizörü ile analiz edilirken, serum 25-(OH) D konsantrasyonu sıvı kromatografisi-kütle spektrometrisi ile analiz edildi. SPT ve CPT kan BHBA konsantrasyonlarının kontrol grubunda yükselmiş olduğu bulundu (P<0.001). Gebe toksemi olan keçilerde 25-(OH) D vitamin düzeyi CG'dekinden önemli ölçüde düşük olarak belirlendi (P<0.001). Gebe toksemi olan keçilerde serum demir konsantrasyonu CG'dekinden önemli ölçüde düşük bulundu (P<0.010). Serum 25-(OH) D'nin gebelik toksemisinin tanısında duyarlılığı % 90, özgüllüğü % 95, eşik değeri 26,12 ve AUC değeri 0,95 olarak bulundu. Keçilerde gebelik toksemisinin 25-(OH) D ve BHBA ile güçlü negatif korelasyon, glikoz ve demir ile anlamlı pozitif korelasyon gösterdiği görüldü. Bu çalışmada gebe toksemi olan keçilerde 25-(OH) D düzeylerinin önemli ölçüde azaldığı ve inflamasyonla belirgin bir korelasyon gösterdiği sonucuna varılmıştır.

Keywords

keçi , 25-OH , bhba , demir

Ethical Statement

The study had commenced following approval from the B.Ü Regional Ethics Committee (Meeting No: 2024/01, Decision No: 01/03).

Supporting Institution

This study has been financially supported by TÜBITAK-BİDEB-2209-a (Project Number: 1919B012323573).

Project Number

2024/1-1919B012323573

Thanks

We would like to thank TÜBİTAK BİDEB (2024/1-1919B012323573) for their financial support of this research.

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