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EXPERIMENTAL INVESTIGATION OF THE EFFECTS OF PROBIOTIC SUPPLEMENTATION ON OBESITY AND INFLAMMATION IN OBESE RATS

Year 2017, , 44 - 50, 22.12.2017
https://doi.org/10.23884/ijhsrp.2017.2.2.02

Abstract

TNF-α levels
of antiinflammatory and proinflammatory markers were used to investigate
effects Obesity is a disease that occurs when body
has many endocrine and metabolic functions and fat tissue is higher than
normal. There is strong relationship between obesity and inflammation due to
production of numerous lipid molecules called adipokines, such as leptin, tumor
necrosis factor (TNF-α), interleukins and adiponectin. It was aimed to study
obesity and effect on inflammation in experimentally obese animal model study
apllied probiotic supplementation. Three different test animal groups
were formed. For this purpose; IL-10 and TNF-α on inflammation; leptin levels
were used for investigating effect on development of obesity and total
cholesterol, HDL and LDL levels from lipid profiles were planned to examine. In
obtained serum samples, these parameters were determined by sandwich ELISA
technique with commercially available kits.  Mean weights at the beginning of study
and at the end of 8 weeks were 240,2±9,7 and 283,8±11,0  gr in first, second and third groups;
254,6±16,0 and 307,0±14,5 gr; 330,0±48,6 and 400,5±65,0 gr respectively. At the
beginning and end of the probiotic use, weight averages were 288,6±9,9 and
311,8±17,1 gr in first, second and third groups 313.4±17.1 and 339.6±19.7 gr;
412,0±67,7 and 422,0±71,1 gr respectively. When weights were evaluated before
and after probiotic use of third group, weight gain was decreased. Although
there was no significant difference in HDL, LDL, IL-10 and leptin levels among
all group, there was significant difference in total cholesterol and TNF-α
values (p<0,05). Compared to first group and second group, there was significant
difference in total cholesterol and TNF-α values, in second and third groups
there was significant difference in total cholesterol values (p<0,05). As result; probiotics are recognized as living
organisms when taken in sufficient quantities, affect health of environment
positively, and these beneficial effects are considered to extend to obesity
inflammatory diseases. Probiotic supplementation of normal diet may be healthy
approach to prevention of various diseases. In order to spread this application
to society as whole, it is necessary to increase number of works.

References

  • [1] Escobedo, N., Oliver, G., The Lymphatic Vasculature: Its Role in Adipose Metabolism and Obesity, Cell Metabolism, 26 (2017), 4, pp. 598-609.
  • [2] Gnacinska, M., Malgorzewicz, S., Stojek, M., Lysiak-Szydlowska, W., Sworczak, K., Role of adipokines in complications related to obesity. A review, Advances in medical sciences, 54 (2009), 2, pp. 150-157.
  • [3] Aktaş, G., Şit, M., Tekçe, H., Yeni adipokinler: Leptin, adiponektin ve omentin, Abant Medical Journal, 2 (2013), 1, pp. 56-62.
  • [4] Dragano, N. R., Haddad-Tovolli, R., Velloso, L. A., Leptin, Neuroinflammation and Obesity, In Endocrine Immunology, 48 (2017), pp. 84-96.
  • [5] Ouchi, N., Parker, J. L., Lugus, J. J., Walsh, K., Adipokines in inflammation and metabolic disease, Nature Reviews Immunology, 11 (2011), 2, pp. 85-97.
  • [6] Zimmermann, P., Curtis, N., The influence of probiotics on vaccine responses–A systematic review, Vaccine, (2017).
  • [7] Cox, A. J., West, N. P., Cripps, A. W., Obesity, inflammation, and the gut microbiota, The lancet Diabetes & endocrinology, 3 (2015), 3, pp. 207-215.
  • [8] Plaza-Diaz, J., Gomez-Llorente, C., Fontana, L., Gil, A., Modulation of immunity and inflammatory gene expression in the gut, in inflammatory diseases of the gut and in the liver by probiotics, . World Journal of Gastroenterology: WJG, 20 (2014), 42, pp. 15632-15649.
  • [9] Moise, A. M. R., The Gut Microbiome: Exploring the Connection between Microbes, Diet, and Health, ABC-CLIO, 2017.
  • [10] Delzenne, N. M., Neyrinck, A. M., Bäckhed, F., Cani, P. D., Targeting gut microbiota in obesity: effects of prebiotics and probiotics, Nature Reviews Endocrinology, 7 (2011), 11, pp. 639-646.
  • [11] Hussain, S. S., Bloom, S. R., The regulation of food intake by the gut-brain axis: implications for obesity, International Journal of Obesity, 37 (2013), 5, pp. 625-633.
  • [12] Yoo, S. R., Kim, Y. J., Park, D. Y., Jung, U. J., Jeon, S. M., Ahn, Y. T., Choi, M. S., Probiotics L. plantarum and L. curvatus in Combination Alter Hepatic Lipid Metabolism and Suppress Diet Induced Obesity. Obesity, 21 (2013), 12, pp. 2571-2578.
  • [13] An, H. M., Park, S. Y., Lee, D. K., Kim, J. R., Cha, M. K., Lee, S. W., Ha, N. J., Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats, Lipids in health and disease, 10 (2011), 1, pp. 1-8.
  • [14] Cano, P. G., Santacruz, A., Moya, Á., Sanz, Y., Bacteroides uniformis CECT 7771 ameliorates metabolic and immunological dysfunction in mice with high-fat-diet induced obesity, PloS one, 7 (2012), 7, e41079.
  • [15] Park, D. Y., Ahn, Y. T., Park, S. H., Huh, C. S., Yoo, S. R., Yu, R., Choi, M. S., Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity, PloS one, 8 (2013), 3, e59470.
Year 2017, , 44 - 50, 22.12.2017
https://doi.org/10.23884/ijhsrp.2017.2.2.02

Abstract

References

  • [1] Escobedo, N., Oliver, G., The Lymphatic Vasculature: Its Role in Adipose Metabolism and Obesity, Cell Metabolism, 26 (2017), 4, pp. 598-609.
  • [2] Gnacinska, M., Malgorzewicz, S., Stojek, M., Lysiak-Szydlowska, W., Sworczak, K., Role of adipokines in complications related to obesity. A review, Advances in medical sciences, 54 (2009), 2, pp. 150-157.
  • [3] Aktaş, G., Şit, M., Tekçe, H., Yeni adipokinler: Leptin, adiponektin ve omentin, Abant Medical Journal, 2 (2013), 1, pp. 56-62.
  • [4] Dragano, N. R., Haddad-Tovolli, R., Velloso, L. A., Leptin, Neuroinflammation and Obesity, In Endocrine Immunology, 48 (2017), pp. 84-96.
  • [5] Ouchi, N., Parker, J. L., Lugus, J. J., Walsh, K., Adipokines in inflammation and metabolic disease, Nature Reviews Immunology, 11 (2011), 2, pp. 85-97.
  • [6] Zimmermann, P., Curtis, N., The influence of probiotics on vaccine responses–A systematic review, Vaccine, (2017).
  • [7] Cox, A. J., West, N. P., Cripps, A. W., Obesity, inflammation, and the gut microbiota, The lancet Diabetes & endocrinology, 3 (2015), 3, pp. 207-215.
  • [8] Plaza-Diaz, J., Gomez-Llorente, C., Fontana, L., Gil, A., Modulation of immunity and inflammatory gene expression in the gut, in inflammatory diseases of the gut and in the liver by probiotics, . World Journal of Gastroenterology: WJG, 20 (2014), 42, pp. 15632-15649.
  • [9] Moise, A. M. R., The Gut Microbiome: Exploring the Connection between Microbes, Diet, and Health, ABC-CLIO, 2017.
  • [10] Delzenne, N. M., Neyrinck, A. M., Bäckhed, F., Cani, P. D., Targeting gut microbiota in obesity: effects of prebiotics and probiotics, Nature Reviews Endocrinology, 7 (2011), 11, pp. 639-646.
  • [11] Hussain, S. S., Bloom, S. R., The regulation of food intake by the gut-brain axis: implications for obesity, International Journal of Obesity, 37 (2013), 5, pp. 625-633.
  • [12] Yoo, S. R., Kim, Y. J., Park, D. Y., Jung, U. J., Jeon, S. M., Ahn, Y. T., Choi, M. S., Probiotics L. plantarum and L. curvatus in Combination Alter Hepatic Lipid Metabolism and Suppress Diet Induced Obesity. Obesity, 21 (2013), 12, pp. 2571-2578.
  • [13] An, H. M., Park, S. Y., Lee, D. K., Kim, J. R., Cha, M. K., Lee, S. W., Ha, N. J., Antiobesity and lipid-lowering effects of Bifidobacterium spp. in high fat diet-induced obese rats, Lipids in health and disease, 10 (2011), 1, pp. 1-8.
  • [14] Cano, P. G., Santacruz, A., Moya, Á., Sanz, Y., Bacteroides uniformis CECT 7771 ameliorates metabolic and immunological dysfunction in mice with high-fat-diet induced obesity, PloS one, 7 (2012), 7, e41079.
  • [15] Park, D. Y., Ahn, Y. T., Park, S. H., Huh, C. S., Yoo, S. R., Yu, R., Choi, M. S., Supplementation of Lactobacillus curvatus HY7601 and Lactobacillus plantarum KY1032 in diet-induced obese mice is associated with gut microbial changes and reduction in obesity, PloS one, 8 (2013), 3, e59470.
There are 15 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Article
Authors

Gül Eda Kılınç

Mehtap Ünlü Söğüt

Publication Date December 22, 2017
Submission Date November 15, 2017
Acceptance Date November 27, 2017
Published in Issue Year 2017

Cite

IEEE G. E. Kılınç and M. Ünlü Söğüt, “EXPERIMENTAL INVESTIGATION OF THE EFFECTS OF PROBIOTIC SUPPLEMENTATION ON OBESITY AND INFLAMMATION IN OBESE RATS”, IJHSRP, vol. 2, no. 2, pp. 44–50, 2017, doi: 10.23884/ijhsrp.2017.2.2.02.

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