INVESTIGATION OF ENDOPLASMIC RETICULUM SIGNAL PROTEINS IN CUMULUS CELLS WITH NORMAL, HYPERRESPONSIVE, HYPORESPONSIVE AND POLYCYSTIC OVARY SYNDROMES

Year 2019, , 74 - 83, 27.08.2019

Bahar Kartal , Mehmet Tahir Hatipoğlu Ümit Ali Kayışlı Sevtap Hamdemir Kılıç Evrim Ünsal Candan Özoğul

https://doi.org/10.23884/ijhsrp.2019.4.2.01

Abstract

The aim of the study
was to
investigate Endoplasmic reticulum dependent Unfolded Protein Response signal molecules in cumulus cells of the patients
presenting normal, hyperresponsive, hyporesponsive, and polycystic ovary
syndrome . Cumulus
cells were
provided during oocyte retrieval. 
Of the patients applying for in vitro fertilization treatment, three
subtypes of patient groups were  distinguished
according to their response to follicle stimulations. The first group composed
of normoresponsive (n=8) was considered as retrieval of 10-20 oocytes. The
second group composed of hyporesponsive (n=8) as retrieval of 6 or less
oocytes. Retrieval of 30 or more oocytes as a response to the same stimulus was
considered hyperrespponsive (n=8), which included  polycyctıc ovary sydromes. (n=8). We analyzed
the protein expressions  of glucose
regulated protein 78 and mRNAs levels  of
the X-box binding protein-1 and splicing 
X-box binding protein-1 by Western blot and Reverse Transcriptase PCR,
respectively ,in the CCs from different patient groups. All data were loaded to
the software Sigma Stat 3. Differences between groups were evaluated with
one-way ANOVA post hoc TURKEY test. We  found that the protein expression of  glucose regulated protein 78 was two fold
higher in cumulus cells  from hyporesponsive group than the other
groups. Reverse
Transcriptase PCR results
showed us, the mRNA expression of  splicing  X-box / X-box binding protein-1 level was 1,5
fold higher in the cumulus
cells  from the hyporesponsive group compared to
others. Furthermore, we have shown that
when the cumulus cells were
exposed to  signal pathway molecules that
related with ER stress; a decrease in the cell proliferation and/or increase in
apoptosis can occured. We have shown that an increased ER stress in the cumulus cells of the ovarian follicle from the
patients forming the hyporesponsive group. We can concluded that increased ER stress or impaired protein folding mechanism in
cumulus cells  may affect oocyte
maturation  therefore  the agents  may 
be  used  to decrease 
pathological ER stress in the hyporesponsive patients. 

Keywords

Endoplasmic Reticulum Stress, Unfolded Protein Response, INVESTIGATION

Supporting Institution

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors

Project Number

This research received no specific grant from any funding agency in the public, commercial or not-for-profit sectors

Thanks

We gratefully acknowledge Gen Art IVF Center for supplying cumulus cells.

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