IMMUNOHISTOCHEMICAL ANALYSIS OF SFLT-1 AND ADAMTS-8 EXPRESSION IN DIABETIC RAT TESTIS TISSUE

Year 2019, Volume: 4 Issue: 3, 225 - 232, 13.12.2019

Seval Kaya , Yusuf Nergiz , Fırat Asır , Ebru Gökalp Özkorkmaz , Gamze Erdogan , Engin Deveci

https://doi.org/10.23884/ijhsrp.2019.4.3.07

Abstract

Abstract: The aim of this study was to determine the testicular inflammation
and angiogenetic effect of diabetes with ADAMTS-8 and SFlt-1 proteins
immunohistochemically. Wistar albino male rats (n: 12)were used for the study,
Group 1: Control group (n: 6), only 1 ml i.p. saline injection was performed.
Group 2: Diabetes group (n: 6) received a single intraperitoneal dose of
streptozotocin (STZ) of 60 mg / kg. The glucose value measured above 250 mg /
dl was considered as diabetic. Under anesthesia, dissection was performed at
the lower part of abdominal clearance and testicular tissue was removed. Testis
tissues were fixed in 10% neutral formalin, followed by the routine paraffin
protocol and cut with a microtome. Then, primary antibodies (ADAMTS-8, sFlt-1)
were applied by immunohistochemistry method and incubated at +4 ˚C overnight.
The sections were then examined under a light microscope.

Diabetes
group showed that Leydig cells in the  inter tubular area had vacuolization and
capillary dilatations in histopathological examinations. sFlt-1 staining of
control group showed positive expression in capillary endothelium between
Leydig chambers of the intertubular area. Sflt-1 expression of diabetes group
was observed in degenerative spermatic cells and Sertoli cells of the basement
membrane facing tubules. The expression of ADAMTS-8in control group was
positive in some Leydig cells in the interstitial area of Sertoli cells in
seminiferous tubule but, spermatogenetic cells were negative. In the ADAMTS-8
staining of diabetes group, the expression of ADAMTS-8 was increased in the
stromal cells and some inflammatory cells in the intertubular space. SFlt-1 plays a crucial role in angiogenesis
as well as in diabetic testes and is marked as a precursor for the disruption
of vascular structure and blood flow due to degenerative changes. It is thought
that the distribution of ADAMTS-8 may be a determinant protein in the
development of extracellular matrix and in damage to testicular tissue of
diabetic testis.

Keywords

diabetes, testis, ADAMTS-8, sFlt-1, immunohistochemistry

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