Cell Culture Test and Bacterial Growth Evaluation of NiTiNbX ( X=Ta and V) Biocompatible Shape Memory Alloys in Simulated Body Fluid

Year 2022, , 59 - 64, 28.06.2022

Esra Balci , Cennet Canan Karaderi , Hüseyin Kahraman , Fethi Dağdelen

https://doi.org/10.46460/ijiea.1057497

Abstract

In this study, a quaternary biocompatible shape memory alloy (BSMA) was produced by adding different ratios of Ta and V elements to the equiatomic NiTi and Ni27Ti50Nb23 ternary alloy. Prepared alloys; They were coded as Ni50Ti50 (EB1), Ni27Ti50Nb23 (EB2), Ni27Ti50Nb22Ta1 (E1), Ni27Ti50Nb20Ta3 (E3), Ni27Ti50Nb18Ta5 (E5), Ni27Ti50Nb22V1 (B1), Ni27Ti50Nb21V2 (B2) ve Ni27Ti50Nb18V5 (B5). The BSMA samples prepared to be used for this experiment were cut in regular and equal sizes and subjected to sterilization processes. Simulated body fluid (SBF), in which the prepared alloys were in contact, was prepared in the laboratory and used in in-vitro studies. In this study, a closed incubator system was preferred and a pathogenic bacterium, Pseudomonas aeruginosa (ATCC 27853), was used. For the production of bacteria, Nutrient agar solid was prepared. Bacterial cell density grown in the artificial body fluid with the control (simulated body fluid); determined for each alloy using a spectrophotometer (OD600). In the other stage; bacteria were cultivated from liquid culture (SBF containing Pseudomonas aeruginosa) to petri dishes containing solid media by smear method for each alloy. At the end of this period, bacterial colonies were observed with an ultraviolet (UV) lamp. The degree of biocompatibility was evaluated according to the results of the cell culture test performed for examination. It was observed that the cell density values of NiTiNbTa BSMAs added with Ta element were lower than the control (SBF). In addition, much lower values were determined in the alloys prepared with the increase of vanadium element contribution in NiTiNbV BSMA. According to the bacterial growth tests obtained, it can be said that the Ta element increases the biocompatibility in NiTi alloys, since fewer bacterial colonies are seen in the Ta element added environments than the others.

Keywords

Biocompatible shape memory alloy, Pseudomonas aeruginosa, In-Vitro, NiTiNbTa, NiTiNbV

NiTiNbX ( X=Ta ve V) Biyouyumlu Şekil Hatırlamalı Alaşımların Yapay Vücut Sıvısı İçerisinde Hücre Kültür Testi ve Bakteri Üreme Değerlendirilmesi

Abstract

Bu çalışmada eş atomlu NiTi ve Ni27Ti50Nb23 üçlü alaşıma farklı oranlarda Ta ve V elementi katkılanarak dörtlü biyouyumlu şekil hatırlamalı alaşım (BŞHA) üretildi. Hazırlanan alaşımlar; Ni50Ti50 (EB1), Ni27Ti50Nb23 (EB2), Ni27Ti50Nb22Ta1 (E1), Ni27Ti50Nb20Ta3 (E3), Ni27Ti50Nb18Ta5 (E5), Ni27Ti50Nb22V1 (B1), Ni27Ti50Nb21V2 (B2) ve Ni27Ti50Nb18V5 (B5) şeklinde kodlandı. Daha sonra BŞHA’lar düzenli ve eş boyutta olmak üzere kesildi ve sterilizasyon işlemlerinden geçirildi. Hazırlanan alaşımların temas halinde bulunmuş olduğu yapay vücut sıvısı (SBF) laboratuvar ortamında hazırlandı ve in-vitro çalışmalarda kullanıldı. Bu çalışmada kapalı inkübatör sistemi tercih edilmiştir ve patojen bir bakteri olan Pseudomonas aeruginosa (ATCC 27853) suşu kullanılmıştır. Bakteri üretimi için, Nutrient agar besiyeri hazırlandı. Kontrol (yapay vücut sıvısı) ile numunelerin temas ettiği yapay vücut sıvısı içerisinde üreyen bakteri hücre yoğunluğu; her bir alaşım için spektrofotometre (OD600) kullanılarak belirlendi. Diğer aşamada ise; katı besi yeri içeren petri kaplarına, sıvı kültüründen (Pseudomonas aeruginosa içeren SBF) her bir alaşım için yayma yöntemi ile bakteri ekimi gerçekleştirildi. Bu süre sonunda, ultraviyole (UV) lambası ile üreyen bakteri kolonileri gözlemlendi. Biyouyumluluk derecesi incelenmesi için yapılan hücre kültür testi sonuçlarına göre değerlendirildi. Ta elementi ile katkılanmış NiTiNbTa BŞHA’ların hücre yoğunluk değerleri kontrol (SBF)’ ye göre daha düşük değerlerde olduğu gözlendi. Ayrıca, NiTiNbV BŞHA’da vanadyum elementi katkısının artması ile hazırlanan alaşımlarda çok daha düşük değerler tespit edilmiştir. Elde edilen bakteriler üreme testlerine göre, Ta elementi katkılı ortamlarda diğerlerine göre daha az bakteri kolonisi görüldüğü için NiTi alaşımlarda, Ta elementinin biyouyumluluğu arttırdığı söylenebilir.

Keywords

Biyouyumlu şekil hatırlamalı alaşım, Pseudomonas aeruginosa, In-Vitro, NiTiNbTa, NiTiNbV

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