PVA/WHEY PROTEIN NANOFIBER-COATED PP MELT BLOWN INTEGRATED WITH PICKERING EMULSION OF CITRAL STABILIZED FOR POTENTIAL MEDICAL APPLICATIONS

Year 2024, , 1 - 7, 29.06.2024

Fatma Nur Parın , Ayşenur Yeşilyurt , Uğur Parın

https://doi.org/10.46460/ijiea.1206901

Abstract

As an antibacterial agent with pleasant fragrance, citral (CIT) indicates hydrophobic character, and therefore has low water solubility. In this study, Pickering emulsions were formed and polyvinyl alcohol (PVA)/whey protein hydrophilic nanofibers were coated on PP melt blown non-woven surfaces by electrospinning method. In this context, hydrophobic citral essential oil is stabilized with β-cyclodextrin (β-CD) in the electrospinning process. PVA and whey protein polymer blend were used as nanofiber matrices. The morphological, physical, and thermal properties of the β-CD/citral complexes were investigated in PVA/whey protein nanofiber-coated PP non-wovens at various β-CD levels (1:2, 1:4 and 1:6). Furthermore, zone inhibition procedure was performed to evaluate antibacterial activity of the samples against Gram (+) (Staphylococcus aureus ATCC® 25923) and Gram (-) (Escherichia coli ATCC® 25922, and Pseudomonas aeruginosa ATCC® 27853) bacteria. The morphology of fibers showed that all obtained nanofiber-coated PP surfaces were in the range with 216 - 330 nm average fiber diameter. Fourier Transform Infrared (FT-IR) and thermal gravimetric analysis (TGA) thermograms revealed that citrals were successfully integrated into the bio-based nanofibers. As the amount of citral increased (i.e., the β-CD/citral increased), the thermal resistance of bio-based nanofiber coated PP surfaces increased. Antibacterial activity indicated the citral-loaded nanofiber-coated PP surfaces were most effective against Escherichia coli, while none of the samples have antibacterial activity against Pseudomonas aeruginosa. Overall, the results displayed that the fabricated PVA/whey protein nanofiber-coated PP samples integrated with Pickering emulsion of citral stabilized have promising wound dressing applications.

Keywords

Antibacterial property, Pickering emulsion, PP melt blown, PVA/whey protein nanofiber, β-CD/citral complex

Supporting Institution

This study did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

POTANSİYEL MEDİKAL UYGULAMALAR İÇİN PVA/WHEY PROTEİN NANOLİF KAPLI PP ERİYİK ÜFLEMELİ DOKUSUZ YÜZEYLERE ENTEGRE EDİLMİŞ SİTRAL STABİLİZE PİCKERİNG EMÜLSİYONLAR

Abstract

Hoş kokulu bir antibakteriyel madde olarak sitral (SIT) hidrofobik karakter sergiler ve bu sebeple suda çözünürlüğü düşüktür. Bu çalışmada Pickering emülsiyonları oluşturulmuş ve elektroçekim yöntemi ile polipropilen (PP) eriyik üflemeli dokusuz yüzeylere polivinil alkol (PVA)/whey proteini hidrofilik nanofiberler kaplanmıştır. Bu bağlamda, elektroçekim yönteminde hidrofobik sitral uçucu yağı β-siklodekstrin (β-CD) ile stabilize edilmiştir. Nanofiber matris olarak PVA ve whey proteini polimer karışımı kullanılmıştır. β-CD/sitral komplekslerinin morfolojik, fiziksel ve termal özellikleri PVA/whey proteini nanofiber kaplı PP dokusuz dokumalarda çeşitli β-CD seviyelerinde (1: 2, 1: 4 ve 1:6) araştırılmıştır. Ayrıca, numunelerin Gram (+) (Staphylococcus aureus ATCC ® 25923) ve Gram (-) (Escherichia coli ATCC ® 25922 ve Pseudomonas aeruginosa ATCC ® 27853) bakterilerine karşı antibakteriyel aktivitesini değerlendirmek için zon inhibisyon prosedürü uygulanmıştır. Liflerin morfolojisi, elde edilen tüm nanofiber kaplı PP yüzeylerin 216 - 330 nm ortalama lif çapı aralığında olduğunu göstermiştir. Fourier Dönüşümü Kızılötesi (FT-IR) spektroskopisi ve termal gravimetrik analiz (TGA) termogramları, sitralin biyo bazlı nanofiberlere başarıyla entegre edildiğini ortaya koymuştur. Sitral miktarı arttıkça (yani β-CD/sitral arttıkça), biyobazlı nanofiber kaplı PP yüzeylerin ısıl direnci artmıştır. Antibakteriyel aktivite, sitral yüklü nanofiber kaplı PP yüzeylerinin Escherichia coli bakterisine karşı en etkili olduğunu gösterirken, örneklerin hiçbirinin Pseudomonas aeruginosa bakterisine karşı antibakteriyel aktivitesi olmadığını göstermiştir. Genel olarak, sonuçlar, sitral stabilize Pickering emülsiyonu ile entegre edilmiş PVA/whey proteini nanofiber kaplı PP örneklerinin umut verici yara pansuman uygulamalarına sahip olduğunu göstermiştir.

Keywords

Antibakteriyel özellik, Pickering emülsiyon, PP melt blown, PVA/whey protein nanolif, β-CD/sitral kompleks

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