ELEMENTAL ANALYSIS AND DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENT OF RHEUM RIBES L. BARK

Year 2024, Volume: 8 Issue: 1, 17 - 21, 29.06.2024

İdris Yolbaş

https://doi.org/10.46460/ijiea.1390339

Abstract

Increasing evidence shows that Rheum ribes bark exhibits therapeutic and pharmacological effects. Therefore, we determined the total phenolic and flavonoid content and performed elemental analysis of R. ribes bark using inductively coupled plasma mass spectrometry. The results indicated a phenolic content of 0,61 ± 0,001 mg gallic acid-equivalent/g sample and a flavonoid content of 0,327 ± 0,026 mg catechin-equivalent/100 g sample. Elemental analysis of R. ribes bark revealed the presence of numerous essential elements, including Fe, K, Ca, Na, Al, and Mg. Our results show that R. ribes bark contains high levels of phenolic and flavonoid compounds and is a source of various important elements. Consequently, it may offer health benefits and might prove to be a valuable resource for the pharmaceutical and food industries.

Keywords

Antioxidant, flavonoid, phenolic, trace element, Rheum ribes

Ethical Statement

NO

Supporting Institution

NO

Thanks

NO

References

• Lindley, M. G. (1998). The impact of food processing on antioxidants in vegetable oils, fruits and vegetables. Trends in Food Science & Technology, 9(8–9), 336–340.
• Yolbaş, İ. (2024). Phenolic Compound Content and Antioxidant Activity of Rheum ribes Shells. Journal of Chemistry, e9151180.
• Güçlü, K., Apak, M. R., & Özyürek, M. (2009). Hidroksil ve Süperoksit Radikallerinin Süpürülmesine Dayalı Yeni Antioksidan Aktivite Tayin Yöntemlerinin Geliştirilmesi, Tübitak Projesi, 1–114.
• Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10), 3954–3962.
• Croom, E. M., & Walker, L. (1995). Botanicals in the pharmacy: new life for old remedies, Medical Economics Company.
• Uyar, B. B., Gezmen-karadağ, M., Şanlıer, N., & Günyel, S. (2013). Toplumumuzda Sıklıkla Kullanılan Bazı Bitkilerin Toplam Fenolik Madde Miktarlarının Saptanması Gıda, 38 (1): 23-29
• Ali, F., Rahul, F. N., Jyoti, S., & Siddique, Y. H. (2017). Health functionality of apigenin: A review. International Journal of Food Properties, 20(6), 1197–1238.
• Kolaç, T., Gürbüz, P., & Yetiş, G. (2017). Phenolic content and antioxidant characteristics of natural products. İÜ Sağlık Hizmetleri Meslek Yüksekokulu Dergisi, 5(1), 1-17.
• Tchounwou, P. B., Yedjou, C. G., Patlolla, A. K., & Sutton, D. J. (2012). Heavy metal toxicity and the environment. Molecular, clinical and environmental toxicology: volume 3: environmental toxicology, 133-164.
• Bal, S. Ş., Kurşat, M., Kuluöztürk, M. F., Karatepe, Ş., & Yilmaz, E. (2019). Determination of the radio-isotope activity concentration in some medicinal and aromatic plants in Bitlis. ALKÜ Fen Bilimleri Dergisi, 183–187.
• Kerdiğe, K. (2019). Karanfil bitkisinde (Syzygium aromaticum) uçucu bileşenlerin ve eser elementlerin analizi (Master's dissertation, Inonu University).
• Kılıç, O. (2020). Trakya bölgesinde yetişen Allium rumelicum, Jurinea kilaea, Peucedanum obtusifolium bitkilerinin toplam fenolik, flavonoid madde, antioksidan ve antimikrobiyal özelliklerinin belirlenmesi (Master's dissertation, Tekirdağ Namık Kemal University).
• Bati, B., Celik, I., Turan, A., Eray, N., Alkan, E. E., & Zirek, A. K. (2023). Effect of isgin (Rheum ribes L.) on biochemical parameters, antioxidant activity and DNA damage in rats with obesity induced with high-calorie diet. Archives of Physiology and Biochemistry, 129(2), 298–306.
• Keser, S., Keser, F., Karatepe, M., Kaygili, O., Tekin, S., Turkoglu, I., Demir, E., Yilmaz, O., Kirbag, S., & Sandal, S. (2020). Bioactive contents, in vitro antiradical, antimicrobial, and cytotoxic properties of rhubarb (Rheum ribes L.) extracts. Natural Product Research, 34(23), 3353–3357.
• Öztürk, M., Aydoğmuş-Öztürk, F., Duru, M. E., & Topçu, G. (2007). Antioxidant activity of stem and root extracts of Rhubarb (Rheum ribes): An edible medicinal plant. Food chemistry, 103(2), 623-630.
• Njume, C., Afolayan, A. J., & Ndip, R. N. (2009). An overview of antimicrobial resistance and the future of medicinal plants in the treatment of Helicobacter pylori infections. Afr. J. Pharm. Pharmacol, 3(13), 685-699.
• Velioğlu, S. Gıda Kimyası-II, Retrieved 12 September, 2023 from https://acikders.ankara.edu.tr/pluginfile.php/57141/mod_resource/content/0/1.A%C3%87IKDERS-GIDA%20K%C4%B0MYASI-II.pdf
• Tosun, E. (2009). Hastalık tedavisinde kullanılan bazı meyve ve sebzelerin dokularında eser element ve mineral tayini (Master's dissertation, Inonu University).
• Gadd, G. M., Griffiths, A. J. Microorganisms and heavy metal toxicity. Microbial Ecology, 4(4), 303–317.
• Eroğlu Samur, G. (2012). Vitaminler Mineraller ve Sağlığımız. Retrieved 12 September 2023 from https://hsgm.saglik.gov.tr/depo/birimler/saglikli-beslenme-ve-hareketli-hayat-db/Dokumanlar/Kitaplar/Vitamin-Mineral-Sagligimiz.pdf
• N. Lajçi, M. Sadiku, X. Lajçi, B. Baruti, ve S. Nikshiq, “Assessment of major and trace elements of fresh water springs in village Pepaj, Rugova region, Kosova”, Journal of International Environmental Application and Science, c. 12, sy 2, ss. 112-120, 2017.
• I. Knerr, H. Blessing, S. Seyferth, R. J. Watling, ve M. A. Chaudhri, “Evaluation of plasma trace element and mineral status in children and adolescents with phenylketonuria using data from inductively-coupled-plasma atomic emission and mass spectrometric analysis”, Annals of Nutrition and Metabolism, c. 63, sy 1-2, ss. 168-173, 2013.
• R. V. Derrell, (1991). “Trace Elements in Human Nutrition Micronutrients in Agriculture, SSSA Book Series 4”. USA, 1991.