Effects of Salicylic Acid Priming Application in Some Switchgrass (Panicum virgatum L.) Cultivars

Abstract

This study aimed to determine the effects of salicylic acid (SA) seed priming applications on the germination parameters of switchgrass (Panicum virgatum L.). The research was carried out in Siirt University, Faculty of Agriculture, Field Crops Laboratory, under controlled conditions at 25±1 °C. The subject of the study consists of the SA priming doses which is (0, 0.10, 0.25, 0.50, 0.75, 1.00 and 1.25 mM) applied to 3 different cultivars of switchgrass (P. virgatum L.) (Kanlow, Shawnee and Trailblazer). The experiment was carried out in Petri dishes according to the randomized complete design with four replications. In the study, germination parameters such as germination percentage, mean germination time, germination index and coefficient of uniformity of germination, and seedling growth parameters such as seedling fresh and dry weight, seedling vigor index were examined. It was detected that all parameters except for seedling fresh weight were statiscally differences among the cultivars (p<0.01). In addition, SA doses significantly affected all germination and seedling growth parameters except seedling fresh weight. To sum up, pre-sowing seed priming with SA can be effective in breaking the seed dormancy and 0.25 mM SA can be used to improve germination and seedling growth in switchgrass to overcome dormancy problem.

Keywords

• Switchgrass
• Cultivars
• Salicylic Acid
• Germination
• Seedling Growth

References

1. Abbasi Khalaki, M., Moameri, M., Asgari Lajayer, B., & Astatkie, T. (2021). Influence of nano-priming on seed germination and plant growth of forage and medicinal plants. Plant Growth Regulation, 93, 13-28. https://doi.org/10.1007/s10725-020-00670-9
2. Açıkbaş, S. (2022). Determination of Forage and Silage Characteristics of Switchgrass (Panicum virgatum L.) Cultivars at Different Harvest Stages. Ph.D. Thesis. The Graduate School of Natural and Applied Science of Siirt University, Siirt, Türkiye.
3. Açıkbaş, S., & Özyazıcı, M. A., (2022). Determination of germination and seedling characteristics of bitter vetch (Vicia ervilia L.) plant under salt stress. 5. International Sciences and Innovation Congress, 11-12 November, Ankara, Türkiye, p. 190-197.
4. Açıkgöz, N., & Açıkgöz, N. (2001). Common mistakes in the statistical analyzes of agricultural experiments I. Single factorials. Anadolu Journal of Aegean Agricultural Research Institute, 11 (1), 135-147.
5. An, C., & Mou, Z. (2011). Salicylic acid and its function in plant immunity. Journal of Integrative Plant Biology, 53 (6), 412-428. https://doi.org/10.1111/j.1744-7909.2011.01043.x
6. Apon, T. A., Ahmed, S. F., Bony, Z. F., Chowdhury, M. R., Asha, J. F., & Biswas, A. (2023). Sett priming with salicylic acid improves salinity tolerance of sugarcane (Saccharum officinarum L.) during early stages of crop development. Heliyon, 9: e16030. https://doi.org/10.1016/j.heliyon.2023.e16030
7. Aravindhakshan, S. C., Epplin, F. M., & Taliaferro, C. M. (2010). Economics of switchgrass and miscanthus relative to coal as feedstock for generating electricity. Biomass and Bioenergy, 34 (9), 1375-1383. https://doi.org/10.1016/j.biombioe.2010.04.017
8. Arslan, M., & Gökkaya, T. H., (2023). Exogenous salicylic acid application during germination of silage maize (Zea mays L.) exposed to PEG-induced drought condition. Turkish Journal of Range and Forage Science, 4 (1), 43-52.

9. Balsamo, R. A., Kelly, W. J., Satrio, J. A., Ruiz-Felix, M. N., Fetterman, M., Wynn, R., & Hagel, K. (2015). Utilization of grasses for potential biofuel production and phytoremediation of heavy metal contaminated soils. International Journal of Phytoremediation, 17 (5), 448-455. https://doi.org/10.1080/15226514.2014.922918
10. Basra, S. M. A., Farooq, M., Wahid, A., & Khan, M. B. (2006). Rice seed invigoration by hormonal and vitamin priming. Seed Science and Technology, 34 (3), 775-780. https://doi.org/10.15258/sst.2006.34.3.23
11. Bewely, J. D., & Black, M. (1994). Seeds: Physiology of Development and Germination. Springer New York, NY.
12. Candoğan, G. Ç., & Geren, H. (2020). A preliminary study on the effect of different nitrogen levels on the forage yield and some agronomical parameters of switchgrass (Panicum virgatum). Journal of Agriculture Faculty of Ege University, 57 (2), 165-172. https://dergipark.org.tr/en/download/article-file/1182119
13. Casler, M. D., Vogel, K. P., Taliaferro, C. M., & Wynia, R. L. (2004). Latitudinal adaptation of switchgrass populations. Crop Science, 44 (1), 293-303. https://doi.org/10.2135/cropsci2004.2930
14. Ceritoğlu, M., & Erman, M. (2020). Mitigation of salinity stress on chickpea germination by salicylic acid priming. International Journal of Agriculture and Wildlife Science, 6 (3), 582-591. https://doi.org/10.24180/ijaws.774969
15. Chakma, R., Biswas, A., Saekong, P., Ullah, H., & Datta, A. (2021). Foliar application and seed priming of salicylic acid affect growth, fruit yield, and quality of grape tomato under drought stress. Scientia Horticulturae, 280, 109904. https://doi.org/10.1016/j.scienta.2021.109904
16. Das, M. K., & Taliaferro, C. M. (2009). Genetic variability and interrelationships of seed yield and yield components in switchgrass. Euphytica, 167, 95-105. https://doi.org/10.1007/s10681-008-9866-3
17. Doğan, M., Tura, A., Odabaşıoğlu, C., Sedetaltun, Y., & Odabaşıoğlu, M. İ. (2021). The effect of salicylic acid on germination and development of soybean (Glycine max. (L.) Merr.) seeds. Firat University Journal of Science, 33 (2), 115-124.
18. Dong, C. J., Wang, X. L., & Shang, Q. M. (2011). Salicylic acid regulates sugar metabolism that confers tolerance to salinity stress in cucumber seedlings. Scientia Horticulturae, 129 (4), 629-636. https://doi.org/10.1016/j.scienta.2011.05.005
19. Duclos, D. V., Altobello, C. O., & Taylor, A. G. (2014). Investigating seed dormancy in switchgrass (Panicum virgatum L.): Elucidating the effect of temperature regimes and plant hormones on embryo dormancy. Industrial Crops and Products, 58, 148-159. https://doi.org/10.1016/j.indcrop.2014.04.011
20. Duclos, D. V., Ray, D. T., Johnson, D. J., & Taylor, A. G. (2013). Investigating seed dormancy in switchgrass (Panicum virgatum L.): Understanding the physiology and mechanisms of coat-imposed seed dormancy. Industrial Crops and Products, 45, 377-387. https://doi.org/10.1016/j.indcrop.2013.01.005
21. El-Hawary, M. M., Hashem, O. S. M., & Hasanuzzaman, M. (2023). Seed priming and foliar application with ascorbic acid and salicylic acid mitigate salt stress in wheat. Agronomy, 13 (2), 493. https://doi.org/10.3390/agronomy13020493
22. Ellis, R. A., & Roberts, E. H. (1981). The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9(2), 373-409.
23. Evers, G. W., & Parson, M. J. (2003). Soil type and moisture level influence on Alamo switchgrass emergence and seedling growth. Crop Science, 43 (1), 288-294. https://doi.org/10.2135/cropsci2003.0288
24. Faisal, S., Muhammad, S., Luqman, M., Hasnain, M., Rasool, A., Awan, M. U. F., Khan, Z. I., & Hussain, I. (2023). Effects of priming on seed germination, physico-chemistry and yield of late sown wheat crop (Triticum aestivum L.). Polish Journal of Environmental Studies, 32 (2), 1113-1124. https://doi.org/10.15244/pjoes/155970
25. Farooq, M., Aziz, T., Basra, S. M. A., Cheema, M. A., & Rehman, H. (2008). Chilling tolerance in hybrid maize induced by seed priming with salicylic acid. Journal of Agronomy and Crop Science, 194 (2), 161-168. https://doi.org/10.1111/j.1439-037X.2008.00300.x
26. Hacisalihoglu, G. (2008). Responses of three switchgrass (Panicum virgatum L.) cultivars to seed priming and differential aging conditions. Acta Agriculturae Scandinavica Section B-Soil and Plant Science, 58 (3), 280-284. https://doi.org/10.1080/09064710701706218
27. Hartman, J. C., Nippert, J. B., Orozco, R. A., & Springer, C. J. (2011). Potential ecological impacts of switchgrass (Panicum virgatum L.) biofuel cultivation in the central great plains, USA. Biomass and Bioenergy, 35 (8), 3415-3421. https://doi.org/10.1016/j.biombioe.2011.04.055
28. Hayat, S., & Ahmad, A. (2007). Salicylic Acid: A Plant Hormone. Springer, Dordrecht, Netherlands.
29. Hayat, S., Fariduddin, Q., Ali, B., & Ahmad, A. (2005). Effect of salicylic acid on growth and enzyme activities of wheat seedlings. Acta Agronomica Hungarica, 53 (4), 433-437. https://doi.org/10.1556/AAgr.53.2005.4.9
30. Haynes, J. G., Pill, W. G., & Evans, T. A. (1997). Seed treatments improve the germination and seedling emergence of switchgrass (Panicum virgatum L.). Hort Science, 32 (7), 1222-1226. https://doi.org/10.21273/HORTSCI.32.7.1222
31. Hu, Z., Fang, Z., Hu, B., Wen, X., Lou, L., & Cai, Q. (2022). Profiling of water-use efficiency in switchgrass (Panicum virgatum L.) and the relationship with cadmium accumulation. Agronomy, 12, 507. https://doi.org/10.3390/agronomy12020507
32. Ilyas, N., Gull, R., Mazhar, R., Saeed, M., Kanwal, S., Shabir, S., & Bibi, F. (2017). Influence of salicylic acid and jasmonic acid on wheat under drought stress. Communications in Soil Science and Plant Analysis, 48 (22), 2715-2723. https://doi.org/10.1080/00103624.2017.1418370
33. Janda, T., Gondor, O. K., Yordanova, R., Szalai, G., & Pál, M. (2014). Salicylic acid and photosynthesis: Signalling and effects. Acta Physiologiae Plantarum, 36 (10), 2537-2546. https://doi.org/10.1007/s11738-014-1620-y
34. Jatana, B. S., Ram, H., & Gupta, N. (2020). Application of seed and foliar priming strategies to improve the growth and productivity of late sown wheat (Triticum aestivum L.). Cereal Research Communications, 48, 383-390. https://doi.org/10.1007/s42976-020-00036-x
35. Jayakannan, M., Bose, J., Babourina, O., Rengel, Z., & Shabala, S. (2015). Salicylic acid in plant salinity stress signaling and tolerance. Plant Growth Regulation, 76 (1), 25-40. https://doi.org/10.1007/s10725-015-0028-z
36. Johnson, S. E., Lauren, J. G., Welch, R. M., & Duxbury, J. M. (2005). A comparison of the effects of micronutrient seed priming and soil fertilization on the mineral nutrition of chickpea (Cicer arietinum), lentil (Lens culinaris), rice (Oryza sativa) and wheat (Triticum aestivum) in Nepal. Experimental Agriculture, 41 (4), 427-448. https://doi.org/10.1017/S0014479705002851
37. Kalsa, K. K., & Abebie, B. (2012). Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp, dasycarpa (Ten.). African Journal of Agricultural Research, 7 (21), 3202-3208.
38. Kesen, Z., & Geren, H. (2020). Effect of different cutting frequencies on the dry matter yield and some forage quality characteristics of switch grass (Panicum virgatum). Journal of Agriculture Faculty of Ege University, 57 (1), 95-103.
39. Khan, W., Prithiviraj, B., & Smith, D. L. (2003). Photosynthetic responses of corn and soybean to foliar application of salicylates. Journal of Plant Physiology, 160 (5), 485-492. https://doi.org/10.1078/0176-1617-00865
40. Latif, F., Ullah, F., Mehmood, S., Khattak, A., Khan, A. U., Khan, S., & Husain, I. (2016). Efects of salicylic acid on growth and accumulation of phenolics in Zea mays L. under drought stress. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 66 (4), 325-332. https://doi.org/10.1080/09064710.2015.1117133
41. Loch, D. S., Adkins, S. W., Heslehurst, M. R., Paterson, M. F., & Bellairs, S. M. (2004). Seed Formation, Development, and Germination. In Warm-season (c4) Grasses. Agronomy Society of America, pp 95-144.
42. Madakadze, I. C., Prithiviraj, B., Madakadze, R. M., Stewart, K., Peterson, P., Coulman, B. E., & Smith, D. L. (2000). Effect of preplant seed conditioning treatment on the germination of switchgrass (Panicum virgatum L.). Seed Science and Technology, 28 (2), 403-411. https://www.cabdirect.org/cabdirect/abstract/20003034673
43. Maghsoudi, K., Emam, Y., Ashraf, M., & Arvin, M. J. (2019). Alleviation of field water stress in wheat cultivars using silicon and salicylic acid applied separately or in combination. Crop and Pasture Science, 70 (1), 36-43. https://doi.org/10.1071/CP18213
44. Marthandan, V., Geetha, R., Kumutha, K., Renganathan, V. G., Karthikeyan, A., & Ramalingam, J. (2020). Seed priming: A feasible strategy to enhance drought tolerance in crop plants. International Journal of Molecular Sciences, 21 (21), 8258. https://doi.org/10.3390/ijms21218258
45. Missaoui, A. M., Paterson, A. H., & Bouton, J. H. (2006). Molecular markers for the classification of switchgrass (Panicum virgatum L.) germplasm and to assess genetic diversity in three synthetic switchgrass populations. Genetic Resources and Crop Evolution, 53, 1291-1302. https://doi.org/10.1007/s10722-005-3878-9
46. Mitchell, R., Fritz, J., Moore, K., Moser, L., Vogel, K., Redfearn, D., & Wester, D. (2001). Predicting forage quality in switchgrass and big bluestem. Agronomy Journal, 93 (1), 118-124. https://doi.org/10.2134/agronj2001.931118x
47. Najafabadi, M. Y., & Ehsanzadeh, P. (2017). Salicylic acid effects on osmoregulation and seed yield in drought-stressed sesame. Agronomy Journal, 109 (4), 1414-1422. https://doi.org/10.2134/agronj2016.11.0655
48. Özkorkmaz, F., & Öner, F. (2022). Determination of the effects of salicylic acid treatments on germination and seed properties of barley (Hordeum vulgare L.) cultivars under salt stress. Ordu University Journal of Science and Technology, 12 (2), 119-134. https://doi.org/10.54370/ordubtd.1143106
49. Öztürk, E., Akay, H., & Sezer, İ. (2021). The effect of salicylic acid pre-application against salt stress during germination and early seedling development in sugar corn. Journal of the Institute of Science and Technology, 11 (4), 3213-3221. https://doi.org/10.21597/jist.953388
50. Özyazıcı, G. (2021). Effects of salicylic acid applications on salt stress in milk thistle (Silybum marianum L.). 3rd International Cukurova Agriculture and Veterinary Congress, Adana, Turkey.
51. Özyazıcı, M. A., & Açıkbaş, S. (2021). The effect of seed priming applications on germination parameters of red clover (Trifolium pratense L.). Journal of the Institute of Science and Technology, 11(4), 3232-3242. https://doi.org/10.21597/jist.992180
52. Parrish, D. J., & Fike, J. H. (2005). The biology and agronomy of switchgrass for biofuels. Critical Reviews in Plant Sciences, 24(5-6), 423-459. https://doi.org/10.1080/07352680500316433
53. Poór, P., Borbély, P., Bódi, N., Bagyánszki, M., & Tari, I. (2019). Effects of salicylic acid on photosynthetic activity and chloroplast morphology under light and prolonged darkness. Photosynthetica, 57 (2), 367-376. https://doi.org/10.32615/ps.2019.040
54. Saberi, M., Shahriari, A., Tarnian, F., Jafari, M., & Safari, H. (2011). Influence of some chemical compounds on germination and early seedling growth of two range species under allelopathic conditions. Frontiers of Agriculture in China, 5, 310-321. https://doi.org/10.1007/s11703-011-1098-y
55. Scott, S. J., Jones, R. A., & Williams, W. A. (1984). Review of data analysis methods for seed germination. Crop Science, 24 (6), 1192-1199. https://doi.org/10.2135/cropsci1984.0011183X002400060043x
56. Shen, Z. -X., Parrish, D. J., Wolf, D. D., & Welbaum, G. E. (2001). Stratification in switchgrass seeds is reversed and hastened by drying. Crop Science, 41 (5), 1546-1551. https://doi.org/10.2135/cropsci2001.4151546x
57. Sher, A., Sarwar, T., Nawaz, A., Ijaz, M., Sattar, A., & Ahmad, S. (2019). Methods of Seed Priming. In Priming and Pretreatment of Seeds and Seedlings (1st ed.), Springer, Singapore, pp. 1-10.
58. Singh, B., & Usha, K. (2003). Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 39, 137-141. https://doi.org/10.1023/A:1022556103536
59. Singh, P. K., & Gautam, S. (2013). Role of salicylic acid on physiological and biochemical mechanism of salinity stress tolerance in plants. Acta Physiologiae Plantarum, 35, 2345-2353. https://doi.org/10.1007/s11738-013-1279-9
60. Sokhansanj, S., Mani, S., Turhollow, A., Kumar, A., Bransby, D., Lynd, L., & Laser, M. (2009). Large‐scale production, harvest and logistics of switchgrass (Panicum virgatum L.)-current technology and envisioning a mature technology. Biofuels, Bioproducts and Biorefining, 3 (2), 124-141. https://doi.org/10.1002/bbb.129
61. Soleymani, A., & Shahrajabian, M. H. (2018). Changes in germination and seedling growth of different cultivars of cumin to drought stress. Cercetări Agronomice în Moldova, 1 (173), 91-100.
62. Souri, M. K., & Tohidloo, G. (2019). Effectiveness of different methods of salicylic acid application on growth characteristics of tomato seedlings under salinity. Chemical and Biological Technologies in Agriculture, 6, 26. https://doi.org/10.1186/s40538-019-0169-9
63. Tischler, C. R., Young, B. A., & Sanderson, M. A. (1994). Techniques for reducing seed dormancy in switchgrass. Seed Science & Technology, 22, 19-26.
64. Torun, H. (2019). Time-course analysis of salicylic acid efects on ROS regulation and antioxidant defense in roots of hulled and hulless barley under combined stress of drought, heat and salinity. Physiologia Plantarum, 165 (2), 169-182. https://doi.org/10.1111/ppl.12798
65. Wang, Y. R., Yu, L., Nan, Z. B., & Liu, Y. L. (2004). Vigor tests used to rank seed lot quality and predict field emergence in four forage species. Crop Sciences, 44 (2), 535-541. https://doi.org/10.2135/cropsci2004.5350
66. Wright, L., & Turhollow, A. (2010). Switchgrass selection as a "model" bioenergy crop: A history of the process. Biomass and Bioenergy, 34 (6), 851-868. https://doi.org/10.1016/j.biombioe.2010.01.030
67. Zar, J. H. (1996). Biostatistical Analysis. 3rd ed. Prentice Hall, New Jersey, USA.
68. Zarnstorff, M. E., Keys, R. D., & Chamblee, D. S. (1994). Growth regulator and seed storage effects of switchgrass germination. Agronomy Journal, 86 (4), 667-672. https://doi.org/10.2134/agronj1994.00021962008600040015x
69. Zhang, R. D., Chang, J. R., Yue, Z. X., Zhou, Y. F., Liang, X. H., Guo, W., & Cao, X. (2023). Salicylic acid priming promotes sorghum germination under drought stress: Evidence from comparative metabolomics analysis. Applied Ecology and Environmental Research, 21 (4), 3643-3658. http://dx.doi.org/10.15666/aeer/2104_36433658