In Vitro Efficacy of Some Medicinal Plant-Extracts Against Curvularia lunata Causing Blight Disease of Solanum nigrum L.

Abstract

Fungi are both harmful and beneficial to plants they are the causative agents of many plant diseases through the use of diverse strategies. Sometimes they kill their host plants thus there is a need to control the fungi for proper growth and development of plants. Curvularia lunata (Wakker) Boedijn is a causative pathogen of many medicinal plant diseases, especially leaf spot and blight disease of Solanum nigrum L. The present study was conducted to determine the inhibitory effects of six selected medicinal plant extracts viz. Ocimum sanctum L., Solanum melongena L., Bergera koenigii L., Calotropis gigantean (L.) W.T.Aiton, Justicia adhatoda L., Andrographis paniculata (Burm.f.) Wall. ex Nees against mycelial growth of Curvularia lunata. Both ethanolic extract and aqueous extract show significant inhibition of tested pathogenic fungi as compared to control.

Keywords

• antifungal activity
• Curvularia lunata
• medicinal plants
• phyto-extract.

References

1. Ali, Z., Waheed, A., Sidra, F., Mohsin, N., Waseem, A., Iqra, Y., Horia, H., & Kainat, M. (2023). Medicinal importance of Solanum nigrum Linn; a review.Journal of Plant and Environment, 5, 181-189. https://doi.org/10.33687/jpe.005.02.4576
2. Chowhan, P., & Chakraborty, A. P. (2023). First report of Curvularia lunata (ON246070) causing leaf spot disease of banana from Raiganj, West Bengal, India. Plant Health Progress, 24, 32-36. https://doi.org/10.1094/PHP-05-22-0054-RS
3. Dhankhar, S., Kaur, R., Ruhil, S., Balhara, M., Dhankhar, S., & Chhillar, A. K. (2011). A review on Justicia adhatoda: A potential source of natural medicine. African Journal of Plant Science, 5 (11), 620-627.
4. Ghante, P. H., Kanase, K. M., Markad, H. N., Suryawanshi, A. P., & Chavan, P. G. (2019). In vitro efficacy of phyto-extracts against Fusarium oxysporum f. sp. udum causing wilt disease of pigeonpea. Journal of Pharmacognosy and Phytochemistry, 8 (2), 19-21.
5. Goyal, S., Kumar, S., Rawat, P., & Dhaliwal, N. (2013). Antifungal activity of Calotropis procera towards dermatoplaytes. International Journal of Advances in Pharmacy, Biology and Chemistry, 2 (3), 2277-4688
6. Grubb, A., & Raser-Rowland, A. (2012). The Weed Forager's Handbook. Australia: Hyland House Publishing Pty Ltd. Jain, S. K. (1968). Medicinal Plants. India; Thomson Press. Ltd.
7. Haider, E., Khan, M. A., Atiq, M., Shahbaz, M., & Yaseen, S. (2020). Phytoextracts as management tool against fungal diseases of vegetables. International Journal of Biosciences, 16 (3), 303-314. http://dx.doi.org/10.12692/ijb/16.3.303-314
8. Handral, H. K., Pandith, A., & Shruthi, S. D. (2012). A review on Murraya koenigii: multipotential medicinal plant. Asian Journal of Pharmaceutical and Clinical Research, 5 (4), 5-14.

9. Hoorman, J. J. (2011). The Role of Soil Fungus. Factsheet-Agriculture and natural resources. US; The Ohio State University Extension.
10. Karthikeyan, A., Shanthi, V., & Nagasathaya, A. (2009). Preliminary phytochemical and antibacterial screening of crude extract of the leaf of Adhatoda vasica. L. International Journal of Green Pharmacy, 3, 1.
11. Kharel, R. (2010). Indian traditional herbs Adhatoda vasica and its Medicinal application. Journal of Chemistry, 2 (1), 240-245.
12. Meena, A. K., Yadav, A., & Rao, M. M. (2011). Ayurvedic uses and pharmacological activities of Calotropis procera Linn. Asian Journal of Traditional Medicines, 6 (2), 45-53.
13. Mishra, A. K., Mishra, A., Kehri, H. K., Sharma, B., & Pandey, A. K. (2009). Inhibitory activity of Indian spice plant Cinnamomum zeylanicum extracts against Alternaria solani and Curvularia lunata, the pathogenic dematiaceous moulds. Annals of clinical microbiology and antimicrobials, 8 (1), 1-7.
14. Nagamani , A., Kunwar, I.K. & Manoharachary, C. (2006). Handbook of Soil Fungi, Delhi, India; I K International Publishing House.
15. Nyeem, M. A. B., Rashid, A. M. U., Nowrose, M., & Hossain, M. A. (2017). Solanum nigrum (Maku): A review of pharmacological activities and clinical effects. International Journal of Applied Research, 3 (1), 12-17.
16. Odyuo, T., Simon, S., & Lal, A. A. (2018). Effect of selected botanical extracts against Curvularia Sp. and Meloidogyne incognita (J2). Journal of Pharmacognosy and Phytochemistry, 7 (3), 2331-2336.
17. Okhuarobo, A., Falodun, J. E., Erharuyi, O., Imieje, V., Falodun, A., & Langer, P. (2014). Harnessing the medicinal properties of Andrographis paniculata for diseases and beyond: a review of its phytochemistry and pharmacology. Asian Pacific journal of tropical disease, 4 (3), 213-222. https://doi.org/10.1016/S2222-1808(14)60509-0
18. Parveen, S., Wani, A. H., Bhat, M. Y., Malik, A. R., Koka, J. A., & Ashraf, N. (2017). Antimycotic potential of some phytoextracts on some pathogenic fungi. Journal of Biopesticides, 10 (1), 60-65.
19. Prakash, P. A. G. N., & Gupta, N. (2005). Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: a short review. Indian journal of physiology and pharmacology, 49 (2), 125.
20. Saini, S. C., & Reddy, G. B. S. (2015). A review on curry leaves (Murraya koenigii): versatile multi-potential medicinal plant. American Journal of Phytomedicine and Clinical Therapeutics, 3 (4), 363-368.
21. Saratha, V., & Subramanian, S. P. (2010). Evaluation of antifungal activity of Calotropis gigantea latex extract: An in vitro study. International Journal of Pharmaceutical Sciences and Research, 1(9), 88-96.
22. Sarker, A. K., Ahamed, K., Chowdhury, J. U., & Begum, J. (2009). Characterization of an expectorant herbal basak tea prepared with Adhatoda vasica leaves. Bangladesh Journal of Scientific and Industrial Research, 44 (2), 211-214. https://doi.org/10.3329/bjsir.v44i2.3674
23. Sharma, A., & Kumar, A. (2016). Antimicrobial activity of Justicia adhatoda. World Journal of Pharmceutical Sciences, 5, 1332-13 41. http://dx.doi.org/10.20959/wjpr20167-6571
24. Sharma, K. K., Saikia, R., Kotoky, J., Kalita, J. C., & Devi, R. (2011). Antifungal activity of Solanum melongena L, Lawsonia inermis L. and Justicia gendarussa B. against Dermatophytes. International Journal of Pharm Tech Research, 3 (3), 1635-1640
25. Shekhawat, N., & Vijayvergia, R. (2010). Evaluation of antimicrobial potential of some medicinal plants against plant and human pathogens. Journal of Pharmacy in Research, 3 (4), 700-702.
26. Singh, K. J., & Huidrom, D. (2013). Ethnobotanical uses of medicinal plant, Justicia adhatoda L. by Meitei community of Manipur, India. Journal of Coastal Life Medicine, 1 (4), 322-325.
27. Sitap, M. A., Tilawale, S. R., Nadaf, N. H., & Ghosh, J. S. (2015). Antimicrobial activity of the leaf extracts of Solanum melongena L. (the green variety). International Journal of Pharma Research and Review, 4 (3), 1-5.
28. Wang, S., Lu, Z., Lang, B., Wang, X., Li, Y., & Chen, J. (2022). Curvularia lunata and Curvularia leaf spot of Maize in China. ACS Omega, 7: 47462−47470. https://doi.org/10.1021/acsomega.2c03013
29. Wilhelmus, K. R., & Jones, D. B. (2001). Curvularia keratitis. Transactions of the American Ophthalmological Society, 99, 111.
30. Ying, W. K., Baadu, R., Amiruddin, S., Tan, H. T., Khaw, Y. S., Li, S., & Chong, K. P. (2023). First report of Curvularia lunata causing leaf spot on Oryza sativa in Sabah, Malaysian Borneo. Plant Disease, 107, 2233. https://doi.org/10.1094/PDIS-08-22-1939-PDN