Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae)

Pelin Taştan; Ayten Acar

doi:10.47947/ijnls.1861925

Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae)

Abstract

Lamiaceae family includes approximately 236 genera and 6900 to 7200 species. Medicinal plants belonging to this family have been used for many years in the treatment of various animal and human diseases based on their antibacterial, antioxidant, antitumoral, antifungal, analgesic and insecticidal effects.This research covers the determination of quality control methods and analytical studies for Thymus fallax (catri) and Origanum onites (kekik) which were collected from Şırnak and Izmir, respectively. Heavy metal analyses using Wavelength Dispersive X-Ray Spectrometer (WDXRF) method, determination of ash content and loss on drying according to the European Pharmacopoeia 8.0 standards were carried out. Furthermore, the total phenolic contents of aqueous extracts of both plants were tested with the Folin-Ciocalteu colorimetric method to getting first step for determining their antioxidant activities. According to WDXRF results, the amounts of heavy metals determined were within the limits of pharmacopoeia standards for both species. In addition, total ash and water amounts were determined as 7%, 14% and 14%, 16% for Origanum onites and Thymus fallax respectively and were determined to be in compliance with pharmacopoeia standards. Total phenolic contents (GAE µg/mL) were found to be 28.152 ± 0.389 and 41.009 ± 2.059 for Origanum onites and Thymus fallax, respectively. This is an evidence that both species contain phenolic compounds that will cause them to display antioxidant activity. The data obtained within the scope of the study showed that if the plants are procured correctly and the water extracts are prepared with appropriate methods, the quality and effectiveness will increase the efficiency.

Keywords

Project Number

T6572200

Thanks

This study was supported by the Scientific and Technological Research Council of Türkiye (TÜBİTAK) under the 2209-A Undergraduate Research Projects Support Program (Project No: T6572200).

References

Baser, K. H. C. (2008). Biological and pharmacological activities of carvacrol and carvacrol bearing essential oils. Current Pharmaceutical Design, 14 (29), 3106-3119. https://doi.org/10.2174/138161208786404227
Boros, B., Jakabová, S., Dörnyei, Á., Horváth, G., Pluhár, Z., Kilár, F., & Felinger, A. (2010). Determination of polyphenolic compounds by liquid chromatography–mass spectrometry in Thymus species. Journal of Chromatography A, 1217 (51), 7972–7980. https://doi.org/10.1016/j.chroma.2010.10.010
Cemeroğlu B. (2019). Gıda Analizleri. Tlb Yayınları, 18-23.
Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15 (10), 7313–7352. https://doi.org/10.1016/j.chroma.2010.07.042
Dapkevicius, A., Venskutonis, R., van Beek, T. A., & Linssen, J. P. (1998). Antioxidant activity of extracts obtained by different isolation procedures from some aromatic herbs grown in Lithuania. Journal of the Science of Food and Agriculture, 77 (1), 140-146. https://doi.org/10.1002/(SICI)1097-0010(199805)77
Davis, P. H (Ed.). (1982). Flora of Turkey and the East Aegean Islands (Vol. 7). Edinburgh UK.: Edinburgh University Press.
Davis, P. H., Mill, R. R., & Tan, K. (1988). Flora of Turkey and the East Aegean Islands (Vol. 10, Suppl.). Edinburgh, UK: Edinburgh University Press.
Duman, H., Güner, A., & Özhatay, N. (1995). Origanum L. In A. Güner, N. Özhatay, T. Ekim, & K. H. C. Baser (Eds.), Flora of Turkey and the East Aegean Islands (Vol. 11). Edinburgh, UK: Edinburgh University Press., pp. 207–236.

European Directorate for the Quality of Medicines & HealthCare (EDQM) (2013). European Pharmacopoeia. 8th ed. Strasbourg: Council of Europe.
Güner, A., Aslan, S., Ekim, T., Vural, M. & Babaç, M. T. (Edlr.), (2012). Türkiye Bitkileri Listesi (Damarlı Bitkiler). İstanbul: Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını.
Hossain, M. B., Rai, D. K., Brunton, N. P., Martin-Diana, A.B., & Barry-Ryan, C. (2010). Characterization of phenolic composition in Lamiaceae spices by LC-ESIMS/MS. Journal of Agricultural and Food Chemistry, 58 (19), 10576-10581. https://doi.org/10.1021/jf102042g
Karagözlü, C., & Akbulut, N. (2012). Laboratuvar Tekniği. İzmir: Ege Üniversitesi Ziraat Fakültesi Yayınları, 143.
Kulisic, T., Radonic, A., & Milos, M. (2005). Antioxidant properties of thyme (Thymus vulgaris L.) and wild thyme (Thymus serpyllum L.) essential oils. Italian Journal of Food Science, 17 (3), 315-324.
Marchese, A., Orhan, I. E., Daglia, M., Barbieri, R., Di Lorenzo, A., Nabavi, S. F., & Nabavi, S. M. (2016). Antibacterial and antifungal activities of thymol: A brief review of the literature. Food Chemistry, 210, 402-414. https://doi.org/10.1016/j.foodchem.2016.04.111
Marguí, E., Dalipi, R., Sangiorgi, E., Bival Štefan, M., Sladonja, K., Rogga, V., & Jablan, J. (2022). Determination of essential elements (Mn, Fe, Cu and Zn) in herbal teas by TXRF, FAAS and ICP‐OES. X‐Ray Spectrometry, 51 (3), 204-213. https://doi.org/10.1002/xrs.3241
Morales, R. (2002). The history, botany and taxonomy of the genus Thymus. In E. Stahl-Biskup & F. Sáez (Eds.), Thyme: The genus Thymus London, UK: Taylor & Francis, pp. 1–43.
Moshari-Nasirkandi, A., Alirezalu, A., Alipour, H., & Amato, J. (2023). Screening of 20 species from Lamiaceae family based on phytochemical analysis, antioxidant activity and HPLC profiling. Scientific Reports, 13 (1), 16987. https://doi.org/10.1038/s41598-023-44337-7
Özcan, M. M., & Akbulut, M. (2008). Estimation of minerals, nitrate and nitrite contents of medicinal and aromatic plants. Food Chemistry, 106 (2), 852–858. https://doi.org/10.1016/j.foodchem.2007.06.045
Rice-Evans, C. A., Miller, N. J., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in Plant Science, 2 (4), 152–159. https://doi.org/10.1016/S1360-1385(97)01018-2
Singleton, V. L, & Rossi, J. A. (1965). Colorimetry of Total Phenolic Compounds with Phosphomolybdic-Phosphotungstic Acid Reagents. American Journal of Enology and Viticulture, 16, 144-158. . https://doi.org/10.5344/ajev.1965.16.3.144
Skendi, A., Irakli, M., & Chatzopoulou, P. (2017). Analysis of phenolic compounds in Greek plants of Lamiaceae family by HPLC. Journal of Applied Research On Medicinal And Aromatic Plants, 6, 62-69. https://doi.org/10.1016/j.jarmap.2017.02.001
Skoog, D.A., Holler, F.J., & Crouch, S. R. (2014). Principles of Instrumental Analysis. 6th ed. Belmont (CA): Cengage Learning.
Spyridopoulou, K., Fitsiou, E., Bouloukosta, E., Tiptiri-Kourpeti, A., Vamvakias, M., Oreopoulou, A., & Chlichlia, K. (2019). Extraction, chemical composition and anticancer potential of Origanum onites L. essential oil. Molecules, 24 (14), 2612. https://doi.org/10.3390/molecules24142612
Stahl-Biskup E. (2002). Thyme as a Herbal Drug-Pharmacopoeias and Other Product Characteristics, In: Stahl-Biskup and Saez (Eds.) The genus Thymus. London: Taylor & Francis, 29. https://doi.org/10.4324/9780203216859
Tepe, B., Cakir, A., & Tepe, A. S. (2016). Medicinal uses, phytochemistry, and pharmacology of Origanum onites (L.): A Review. Chemistry & Biodiversity, 13 (5), 504-520. https://doi.org/10.1002/cbdv.201500069
Tzima, K., Brunton, N. P., & Rai, D. K. (2018). Qualitative and quantitative analysis of polyphenols in Lamiaceae plants- A review. Plants, 7 (25), 1-30. https://doi.org/10.3390/plants7020025
World Health Organization. (2007). WHO guidelines for assessing quality of herbal medicines with reference to contaminants and residues. WHO Press.
Yetim, H. (2001). Gıda Analizleri (Ders Notu), Erzurum: Atatürk Üniversitesi Ziraat Fakültesi Ofset Tesisi, 161.

Details

Primary Language

English

Subjects

Botany (Other), Medicinal Plants

Journal Section

Research Article

Authors

Pelin Taştan ^*
0000-0003-0913-5369
Türkiye

Ayten Acar
0009-0003-1413-2741
Türkiye

Publication Date

February 13, 2026

Submission Date

January 13, 2026

Acceptance Date

February 10, 2026

Published in Issue

Year 2026 Volume: 10 Number: 1

DOI

https://doi.org/10.47947/ijnls.1861925

IZ

https://izlik.org/JA43DT56SH

Cite

RIS / Bibtex

APA

Taştan, P., & Acar, A. (2026). Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). International Journal of Nature and Life Sciences, 10(1), 1-9. https://doi.org/10.47947/ijnls.1861925

AMA

1.Taştan P, Acar A. Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). Int J Nature Life Sci. 2026;10(1):1-9. doi:10.47947/ijnls.1861925

Chicago

Taştan, Pelin, and Ayten Acar. 2026. “Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum Onites and Thymus Fallax (Lamiaceae)”. International Journal of Nature and Life Sciences 10 (1): 1-9. https://doi.org/10.47947/ijnls.1861925.

EndNote

Taştan P, Acar A (February 1, 2026) Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). International Journal of Nature and Life Sciences 10 1 1–9.

IEEE

[1]P. Taştan and A. Acar, “Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae)”, Int J Nature Life Sci, vol. 10, no. 1, pp. 1–9, Feb. 2026, doi: 10.47947/ijnls.1861925.

ISNAD

Taştan, Pelin - Acar, Ayten. “Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum Onites and Thymus Fallax (Lamiaceae)”. International Journal of Nature and Life Sciences 10/1 (February 1, 2026): 1-9. https://doi.org/10.47947/ijnls.1861925.

JAMA

1.Taştan P, Acar A. Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). Int J Nature Life Sci. 2026;10:1–9.

MLA

Taştan, Pelin, and Ayten Acar. “Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum Onites and Thymus Fallax (Lamiaceae)”. International Journal of Nature and Life Sciences, vol. 10, no. 1, Feb. 2026, pp. 1-9, doi:10.47947/ijnls.1861925.

Vancouver

1.Pelin Taştan, Ayten Acar. Bioactive Content, Antioxidant Capacity, and Quality Assessment of Origanum onites and Thymus fallax (Lamiaceae). Int J Nature Life Sci. 2026 Feb. 1;10(1):1-9. doi:10.47947/ijnls.1861925