Quantitative Determination of Metabolites of Turkestan Soaproot (Allochrusa gypsophiloides (Regel) Schischk.) Grown in Kazakhstan

Year 2022, Volume: 6 Issue: 2, 129 - 141, 01.12.2022

Valentina Mursalıyeva , Akmaral Algasy , Tlek Mukhanov , Ramazan Mammadov , Nadezhda Gemejiyeva

https://doi.org/10.47947/ijnls.1180197

Abstract

Allochrusa gypsophiloides (Regel) Schischk. (Caryophyllaceae Juss.), Turkestan soaproot (TSR) is a super producer of triterpene saponins with a wide spectrum of pharmacological activity and high surface-active properties. The realization of his full commercial potential requires biochemical studies with a focus on the metabolites dynamics at various phases of plant development and in connection with the storage duration of raw plant material. Spectrophotometric determination of saponins in terms of oleanolic acid was carried out in roots with different morphometric parameters and the aerial part from wild plants collected in the south of Kazakhstan. The content of saponins, carbohydrates, phenols, and flavonoids as well as the foaming properties of ethanol extracts obtained from air-dried TSR roots before and after their long-term storage were evaluated. The maximum quantity of saponins was revealed in average roots from middle-aged and mature generative plants in fruiting. A high level of saponins was detected in the aerial part of plants during the flowering, which decreased twice at the fruiting stage. After long-term storage of the roots, an increase in the initial content of saponins and flavonoids combined with a decrease in carbohydrates was noted. Saponins accumulation was confirmed by a 12-fold increase in the foaming index in the aqueous extract from the deposited roots relative to its initial value in the extract from the original roots. The data obtained indicate the possibility of alternative use of the aerial part of the original plants of A. gypsophiloides at the flowering stage to obtain triterpene saponins.

Keywords

Allochrusa gypsophiloides, Turkestan soaproot, triterpene saponins

Supporting Institution

This research is funded by the Science Committee of the Ministry of Education and Science of the Republic of Kazakhstan

Project Number

No:AP09258985

References

• Balandin, M. F. (1996). Commercial Utilization of Plant-Derived Saponins: An Overview of Medicinal, Pharmaceutical, and Industrial Applications. In: Waller, K., Yamasaki, K. (Eds). Saponins Used in Traditional and Modern Medicine. Plenum Press, New York, pp.1-14. https://doi.org/10.1007/978-1-4899-1367-8
• Bespaev, S. B. (1966). Acanthophyllum gypsophiloides in Kazakhstan: Morphology, Taxonomy, Phytosociology, Testing in Culture. Abstract Thesis on The Scientific Degree of Candidate of Biological Sciences. Alma-Ata, Kazakhstan.
• Böttger, S., & Melzig, M. (2011). Triterpenoid saponins of the Caryophyllaceae and Illecebraceae family. Phytochemistry Letters, 4, 59-68. https://doi.org/10.1016/j.phytol.2010.08.003
• Chirikova, N. K., Olennikov, D. N., & Tankhaeva, L. M. (2010). Quantitative determination of flavonoid content in the aerial part of Baical scullcap (Scutellaria baicalensis Georgi). Russian Journal of Bioorganic Chemistry, 36, 915–922 https://doi.org/10.1134/S1068162010070204
• Chrevach, E., Yudina, T., Frolova, G., Zhivchikova, R. (2009). Dynamics and biological activity saponins in extracts from roots Saponaria officinalis L. Izvestiya vuzov. Food Technology, 4, 25-28. Desai, S., Desai, D., & Kaur, H. (2009). Saponins and their biological activities. Pharma Times, 41(3),13-16.
• Eisenman, S., Strume, D., Zaurov D (2012). Medical Plants of Central Asia: Uzbekistan and Kyrgyzstan. Springer Science & Business Media, USA.
• El Aziz, M. M. A., Ashour, A. S., & Melad, A. S. G. (2019). A review on saponins from medicinal plants: chemistry, isolation, and determination. Journal Nanomedicine Research, 7(4), 282-288. https://doi.org/10.15406/jnmr.2019.08.00199
• El Mabrouki H., Kaukhova, L., Sorokin, V., & Minina, S. (2014). The development technique of quantitative definition saponins in the grass Herniaria glabra L. Belgorod State University Scientific Bulletin Medicine Pharmacy, 24(195), 235-238.
• Faisal, A., & Geelen, D. (2013). Saponins and their role in biological processes in plants. Phytochemical Review, 12, 877-893. https://doi.org/10.1007/s11101-013-9322-4
• Fedotova, V.V., Oganesyan, E. T., & Chelombitko, V. A. (2014). Triterpene glycosides of the herb of Solidago caucasica Kem. Nath. Pharmacy & Pharmacology, 4(5), 52-56. https://doi.org/10.19163/2307-9266-2014-2-4(5)-52-56
• Fedoseeva, L. M., & Bashar, D. B. (2016). Study of saponins in roots of Ferula hormones. Khimiya Rastitel'nogo Syr'ya, 1, 181-184. https://doi.org/10.14258/jcprm.201501122
• Gemejiyeva, N., Mursaliyeva, V., & Mukhanov, T. (2016). Assessment of the current state of Allochrusa gypsophiloides (Regel) Schischk natural populations in the South-Kazakhstan region. News of National Academy of Science of the Republic of Kazakhstan. Series of Biological and Medical, 1(313), 22-29.
• Gladyshev, A., & Mishchenko, A. (1990). Acanthophyllums of Turkmenistan, Their Biology and Prospects for Economic Use. Science, Ashgabat. ISBN 5-8338-0165-6.
• Grudzinskaya, L. M., & Gemejieva, N. G. (2014). List of Medicinal Plants in Kazakhstan. Almaty, Kazakhstan ISBN: 978-601-80248-6-3.
• Guclu-Ustundag, O., & Mazza, G. (2007). Saponins: properties, applications, and processing. Critical Reviews in Food Science and Nutrition, 47, 231-258. https://doi.org/10.1080/10408390600698197
• Khatuntseva, E., Men’shov, V., Shashkov, A., Tsvetkov, Y., Stepanenko, R., Vlasenko, R., … & Nifantiev, N. (2012). Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides Regel. Beilstein Journal of Organic Chemistry, 8, 763-775. https://doi.org/10.3762/bjoc.8.87
• Kondratenko, E. S., Putieva, Z. M., & Abubakirov, N. K. (1981). Triterpene glycosides of plants of the family Caryophyllaceae. Chemistry of Natural Compounds, 17, 303–317. https://doi.org/10.1007/BF01185255
• Kuzmin, E. V., Tugelibaev, G. T., & Sitpaeva, G. T. (2001). Plant Population Reconstruction of Allochrusa gypsophiloides Rgl. in Southern Kazakhstan. (Abiev S, Fursova, N, Veselova, P, Sitpaeva, G, Aidarbayeva, D.) (Eds.). A Study of Kazakhstan Flora and its Protection. Collection of scientific articles. Aidana LLP, Almaty, Kazakhstan, 91-194.
• Kurkin, V. A., Ryazanova, T. K., & Zulkarnyaeva, L. V. (2017) Quantitative determination of saponins in the drugs of Aralia elata. Khimiya Rastitel'nogo Syr'ya, 3, 163-168. https://doi.org/10.14258/jcprm.2017031309
• Kurkina, A. V., Savel’eva, A. E., & Kurkin, V.A. (2022). Development of quantitative determination methods for total flavonoids in Tagetes patula L. herbs. Pharmaceutical Chemistry Journal, 56, 657-660 https://doi.org/10.1007/s11094-022-02691-2
• Lomboeva, S. S., Tankhaeva. L. M., & Olennikov, D. N. (2008). Method for the quantitative determination of the total content of flavonoids in the aerial part of Orthilia secunda (L. House). Khimiya Rastitel'nogo Syr'ya, 2, 65-68.
• Lim, J. G., Park, H. M., & Yoon, K. S. (2020). Analysis of saponin composition and comparison of the antioxidant activity of various parts of the quinoa plant (Chenopodium quinoa Willd.). Food Science & Nutrition, 8, 694-702.
• Mechnikova, G. Ya., Stepanova, T. A., & Zaguzova, E. V. (2007). Quantitative determination of total phenols in strawberry leaves. Pharmaceutical Chemistry Journal, 41(2), 38-41. https://doi.org/10.1007/s11094-007-0021-6
• Mironenko, I. V., Brezhneva, T. A., & Selemenev, V. F. (2011). UV-spectrophotometric determination of triterpene saponins - derivatives of oleanolic acid. Khimiya Rastitel'nogo Syr'ya, 3, 153-157.
• Nielsen, S. S. (2010). Phenol-Sulfuric Acid Method for Total Carbohydrates. (Nielsen S.S.) (Eds). Food Analysis Laboratory Manual. Food Science Texts Series. Springer, Boston, MA., 47-53 https://doi.org/10.1007/978-1-4419-1463-7-6
• Oganesyan, E. T. (1980). Mechanism of the reaction of triterpenoids with sulphuric acid. Chemistry of Natural Compounds, 16, 464-468. https://doi.org/10.1007/BF00571039 Paseshnichenko, V. A. (2001). Plants as producers of bioactive compounds. Soros Educational Journal 7(8), 13-19.
• Pisarev, D. I., Martynova, N. A., Netrebko, N. N., Novikov, O. O., & Sorokopudov, V. N. (2009). Saponins and their definition in the rhizomes Aralia Manchurian from the Belgorod region. Himija Rastitel'nogo Syr'ja, 4, 197-198.
• Putieva, Zh. M., Gorovichu, T. T., Kondratenko, E. S., & Abubakirov, N. K. (1979). Triterpene glycosides of Acanthophllum gypsophiloides VI. Structure of acanthophylloside D. Chemistry of Natural Compounds, 15,148–151. https://doi.org/10.1007/BF00570786
• Rachkovskaya, E., Volkov, E., & Khramtsov, V. (2003). Botanical Geography of Kazakhstan and Middle Asia (Within The Desert Zone). St. Petersburg, 157-162.
• Red Data Book of the Kazakh SSR (1981). Science, Alma-Ata, Kazakhstan, 67-68.
• Singh, B., & Kaur, A. (2018). Control of insect pests in crop plants and stored food grains using plant saponins: a review. Food Science and Technology, 87, 93-101. https://doi.org/10.1016/j.lwt.2017.08.077
• Shadrin, D. M., Pylina Yu. I., Volodina, S. O., & Volodin, V. V. (2011). Chemical analysis of plants Anthyllis vulneraria L. growing in the European Northeast of Russia. Izvestiya of the Samara Scientific Center of the RAS, 13, 1(4), 945-947.
• State Pharmacopoeia of the RF (2010). XII. Vol 2. Moscow, Russia.
• Vincken, J. P., Heng, L., Groot, A de, & Gruppen, H. (2007). Saponins, classification, and occurrence in the plant kingdom. Phytochemistry, 68(3), 275-297. https://doi.org/10.1016/j.phytochem.2006.10.008
• Zhang, J., & Qu, F. (2013). Methods for Analysis of Triterpenoid Saponins. In: Ramawat, K. G., Merillon, J. M. (Eds.) Natural products. Springer-Verlag Berlin Heidelberg, 3311-3323.