Biyoorganik malzeme olarak yeni LAVECN nanokompozitinin sentezi: etkili antimikrobiyal aktivite

Abstract

Medikal, tekstil, tarım ve gıda gibi endüstrilerde kullanılan malzemelerin birçoğu mikroorganizmalara karşı oldukça duyarlıdır. Patojenik mikroorganizmaların neden olduğu enfeksiyonlar, dünya genelinde yıllık anlamlı mortalite vakalarına yol açmakta olup, insan sağlığı açısından ciddi bir tehdit oluşturmaktadır. Bu bağlamda, antimikrobiyal özelliklere sahip malzemelerin kullanımı, endüstriyel uygulamalarda patojenlerle etkin mücadele sağlamak açısından büyük önem arz etmektedir. Bu çalışma, antimikrobiyal aktiviteye sahip yeni LAVECN nanokompozitinin, lesitin, Amanita vaginata, metanol ekstraktı ve kitosan kullanılarak yeşil sentez prensipler-ine uygun, kolay ve ekonomik sentezine dayanmaktadır. LAVECN’in yapısal ve morfolojik özellikleri, Fourier dönüşümü kızılötesi spektroskopisi (FTIR) ve alan emisyon taramalı elektron mikroskobu (FESEM) kullanıla-rak kapsamlı bir şekilde incelenmiş ve tüm öncü bileşenlerin başarılı bir şekilde birleştirildiği ve gözenekli, dü-zensiz bir şekilde bir araya gelmiş bir nanomateryalin oluştuğu doğrulanmıştır. Parçacık boyutu analizinde 226 nm’lik bir çap ortaya çıkmıştır; bu boyut aralığı, biyolojik etkileşimler için uygun kabul edilmektedir. Çalışmada LAVECN nanokompozitinin Staphylococcus aureus, Bacillus subtilis, Escherichia coli ve Candida albicans patojenlerine karşı inhibisyon zonları sırasıyla 25±4.5, 18±1.6, 22±2.1 ve 20±2.4 mm olarak tespit edilmiştir. Ayrıca, Verticillium dahliae ve Fusarium oxysporum gibi bitki patojenlerine karşı %64±4.5 ve %44±4.0’lik yüksek inhibisyon değerleri elde edilmiştir. Sonuç olarak, LAVECN’in geniş spektrumlu anti-mikrobiyal aktivite sergilediği ve bu özelliğiyle biyotıp, gıda muhafazası ve tarımsal hastalık yönetimi alanla-rında potansiyel uygulamaları olan umut verici bir çevre dostu nanomalzeme olabileceği düşünülmektedir.

Keywords

• nanokompozitler
• amanita vaginata
• kitosan
• LAVECN

Synthesis of novel LAVECN nanocomposite as bioorganic material: effective antimicrobial activity

Abstract

Many materials used in industries such as medical, textile, agriculture, and food are highly susceptible to micro-organisms. Infections caused by pathogenic microorganisms lead to significant annual mortality worldwide and pose a serious threat to human health. In this context, the use of materials with antimicrobial properties is of great importance in ensuring effective control of pathogens in industrial applications. This study is based on the easy and economical synthesis of a novel LAVECN nanocomposite with antimicrobial activity, using lecithin, Amanita vaginata, methanol extract, and chitosan, in accordance with green synthesis principles. The structural and morphological properties of LAVECN were comprehensively investigated using Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM), and it was confirmed that all precursor components were successfully combined and a porous, irregularly assembled nanomaterial was for-med. Particle size analysis revealed a diameter of 226 nm; this size range is considered suitable for biological interactions. In this study, the inhibition zones of LAVECN nanocomposite against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Candida albicans pathogens were determined as 25±4.5, 18±1.6, 22±2.1, and 20±2.4 mm, respectively. Furthermore, high inhibition values of 64±4.5% and 44±4.0% were obtained against plant pathogens such as Verticillium dahliae and Fusarium oxysporum. In conclusion, LAVECN exhibits broad-spectrum antimicrobial activity, and with this property, it is considered a promising, environmentally friendly nanomaterial with potential applications in biomedicine, food preservation, and agricultural disease management.

Keywords

• Nanocomposites
• amanita vaginata
• chitosan
• LAVECN

References

1. N. E. El-Naggar, M. H. Hussein, and A. A. El-Sawah, “Bio-fabrication of silver nanoparticles by phycocyanin, characterization, in vitro anticancer activity against breast cancer cell line and in vivo cytotoxicity,” Sci. Rep., vol. 7, no. 1, pp. 1–20, 2017. doi:10.1038/s41598-017-11121-3
2. J. W. Oh, S. C. Chun, and M. Chandrasekaran, “Preparation and in vitro characterization of chi-tosan nanoparticles and their broad-spectrum antifungal action compared to antibacterial activities against phytopathogens of tomato,” Agronomy (Basel), vol. 9, no. 21, 2019. doi.org/10.3390/agronomy9010021
3. W. L. Du, S. S. Niu, Y. L. Xu, Z. R. Xu, and C. L. Fan, “Antibacterial activity of chitosan tri-polyphosphate nanoparticles loaded with various metal ions,” Carbohydr. Polym., vol. 75, no. 3, pp. 385–389, 2009. doi:10.1016/j.carbpol.2008.07.039
4. L. Y. Ing, N. M. Zin, A. Sarwar, and H. Katas, “Antifungal activity of chitosan nanoparticles and correlation with their physical properties,” Int. J. Biomater., vol. 2012, p. 632698, 2012. doi:10.1155/2012/632698
5. I. Frances, S. O. Enejiyon, K. I. Chimbekujwo, O. A. Oyewole, C. O. Adetunji, O. I. Adeyomoye, et al., “Application of chitosan-based nanoparticles as an effective antibacterial agent,” in Chitosan-Based Nanoparticles for Biomedical Applications. Woodhead Publishing, 2025, pp. 195–216. doi:10.1016/B978-0-443-13997-0.00009-6
6. M. Rai, A. Ingle, S. Gaikwad, I. Gupta, A. Yadav, A. Gade, et al., “Fungi: myconano-factory, mycoremediation and medicine,” in Fungi. CRC Press, 2018, pp. 201–219. Doi:10.1201/9781315369471
7. J. Bérdy, “Bioactive microbial metabolites,” J. Antibiot. (Tokyo), vol. 58, no. 1, pp. 1–26, Jan. 2005. doi:10.1038/ja.2005.1
8. M. A. Alghuthaymi, H. Almoammar, M. Rai, E. Said-Galiev, and K. A. Abd-Elsalam, “Myconanoparticles: Synthesis and their role in phytopathogens management,” Biotechnol. Biotechnol. Equip., vol. 29, no. 2, pp. 221–236, Mar. 4 2015. doi:10.1080/13102818.2015.1008194

9. G. Baskar, J. Chandhuru, K. S. Fahad, and A. S. Praveen, “Mycological synthesis, characterization and antifungal activity of zinc oxide nanoparticles,” Asian J Pharm Technol, vol. 3, no. 4, pp. 142–146, 2013.
10. A. Yıldırım and H. Acay, "Harmful dye adsorption via chitosan-lecithin-pleurotus eryngii extract bionanosorbent kinetic investigations," International Journal of Pure and Applied Sciences, vol. 10, 2024. doi: 10.29132/ijpas.1539646
11. H. Acay, A. Yıldırım, İ. G. Güney, and S. Derviş, “Morchella esculenta‐based chitosan biona-nocomposites: Evaluation as an antifungal agent,” J. Food Process. Preserv., vol. 46, no. 11, p. e17117, 2022. doi:10.1111/jfpp.17117
12. K. Bhardwaj, A. Sharma, N. Tejwan, S. Bhardwaj, P. Bhardwaj, E. Nepovimova, et al., “Pleurotus macrofungi-assisted nanoparticle synthesis and its potential applications: A review,” J. Fungi (Basel), vol. 6, no. 4, p. 351, Dec. 9 2020. doi:10.3390/jof6040351
13. Ö. Erdoğan, S. Paşa, and O. Cevik, “Green synthesis and characterization of anticancer effected silver nanoparticles with silverberry (Elaeagnus angustifolia) fruit aqueous extract,” International Journal of Pure and Applied Sciences, vol. 7, no. 3, pp. 391–400, 2021. doi:10.29132/ijpas.915005
14. P. Bordes, E. Pollet, and L. Avérous, “Nano-biocomposites: Biodegradable polyster/nanoclay systems,” Prog. Polym. Sci., vol. 34, no. 2, pp. 125–155, 2009.doi:10.1016/j.progpolymsci.2008.10.002
15. C. O. Dimkpa, J. E. McLean, D. E. Latta, E. Manangón, D. W. Britt, W. P. Johnson, et al., “CuO and ZnO nanoparticles: Phytotoxicity, metal speciation, and induction of oxida-tive stress in sand-grown wheat,” J. Nanopart. Res., vol. 14, no. 9, pp. 1–15, 2012. doi:10.1007/s11051-012-1125-9
16. M. Kumari, A. Mukherjee, and N. Chandrasekaran, “Genotoxicity of silver nanoparticles in Allium cepa,” Sci. Total Environ., vol. 407, no. 19, pp. 5243–5246, Sep. 15 2009. doi:10.1016/j.scitotenv.2009.06.024
17. W. M. Lee, J. I. Kwak, and Y. J. An, “Effect of silver nanoparticles in crop plants Phaseolus radiatus and Sorghum bicolor: Media effect on phytotoxicity,” Chemosphere, vol. 86, no. 5, pp. 491–499, Feb. 2012. doi:10.1016/j.chemosphere.2011.10.013
18. L. Qi, Z. Xu, X. Jiang, C. Hu, and X. Zou, “Preparation and antibacterial activity of chitosan nanoparticles,” Carbohydr. Res., vol. 339, no. 16, pp. 2693–2700, Nov. 15 2004. doi:10.1016/j.carres.2004.09.007
19. H. Acay, İ. Güler Güney, A. Yildirim, S. Derviş, and E. Dereli, “Green synthesis of Pleurotus eryn-gii-derived nanomaterials for phytopathogen control,” Chem. Biodivers., vol. 21, no. 12, p. e202401972, Dec. 2024. doi:10.1002/cbdv.202401972
20. C. M. Christensen, Edible Mushrooms, 2nd ed. United States of America; 1981, p. 118. [Publisher]
21. H. Acay, A. Dündar, S. Kaçar, M. F. Baran, and C. Keskin, “Investigation of nutritional content, antioxidant anticancer and antimicrobial activities of Pleurotus eryngii (DC. Ex Fr.) Quel, extract obtained by different solvents,” KSU J Agric Nat, vol. 24, no. 2, pp. 267–277, 2021. doi:10.18016/ksutarimdoga.vi.742646
22. A. Rafiee, N. Mozafari, N. Fekri, M. Memarpour, and A. Azadi, “Preparation and characterization of a nanohydroxyapatite and sodium fluoride loaded chitosan-based in situ forming gel for enamel biomineralization,” Heliyon, vol. 10, no. 2, p. e24217, Jan. 7 2024. doi: 10.1016/j.heliyon.2024.e24217
23. M. H. Wolf, N. Izaguirre, B. Pascual-José, R. Teruel-Juanes, J. Labidi, and A. Ribes-Greus, “Dielectric characterisation of chitosan-based composite membranes containing fractionated kraft and organosolv lignin,” React. Funct. Polym., vol. 196, p. 105833, 2024. doi:10.1016/j.reactfunctpolym.2024.105833
24. D. S. da Costa, R. M. R. Costa, K. P. Takeuchi, and A. S. Lopes, “Technological parameters of cassava starch/carboxymethyl cellulose blend-based films added of soy lecithin and tocopherol mix,” Polym Test, vol. 129, p. 108245, 2023. doi.org/10.1016/j.polymertesting.2023.108245
25. D. Yadav, K. H. Prashanth, and P. S. Negi, “Low molecular weight chitosan from Pleurotus ostreatus waste and its prebiotic potential,” Int. J. Biol. Macromol., vol. 267, p. 131419, 2024. doi.org/10.1016/j.ijbiomac.2024.131419
26. A. Yıldırım, “Removal of the anionic dye reactive orange 16 by chi-tosan/tripolyphosphate/mushroom,” Chem. Eng. Technol., vol. 44, no. 8, pp. 1371–138, 2021. doi:10.1002/ceat.202100077
27. X. Chen, Y. Gong, K. Kang, Y. Wang, J. Li, Z. Li, T. Wu, and G. Zhang, “Enhancing properties of Li₂TiO₃/Li₄SiO₄ tritium breeding ceramics by chitosan addition,” Nucl. Mater. Energy, vol. 37, Art. no. 101515, 2023, doi: 10.1016/j.nme.2023.101515.
28. T. A. Nyeem, M. E. Hoque, A. A. Tina, M. S. Zawad, and N. K. Mazumder, “Extraction and characterization of chitosan from local fish scales in Bangladesh,” Next Mater., vol. 9, Art. no. 101227, 2025, doi:10.1016/j.nxmate.2025.101227.
29. M. Xavier, S. K. Ghosh, B. B. Nayak, A. K. Balange, N. K. Mehta, and L. Narasimhamurthy, “Development and techno-functional evaluation of ready-to-serve sous vide shrimp in chitosan-spice mix dispersion,” Future Foods, Art. no. 100869, 2025.
30. B. A. Chaithra, D. A. Pooja, H. D. Priyanka, M. H. Harshitha, V. Kamat, S. M. Anush, and K. Prashantha, “Magnetic chitosan Schiff base functionalized with MoS₂ for the effective removal of Cu(II) and Cr(VI) ions from aqueous solution,” Hybrid Adv., vol. 11, Art. no. 100556, 2025, doi:10.1016/j.hybadv.2025.100556.
31. S. Mork, C. Eilertsen, N. Škalko-Basnet, and M. W. Jøraholmen, “Formulation matters: Assessment of the correlation between mucoadhesiveness and type of chitosan formulation for vaginal application,” J. Drug Deliv. Sci. Technol., vol. 114, pt. B, Art. no. 107564, 2025.
32. M. Chandrasekaran, K. D. Kim, and S. C. Chun, “Antibacterial activity of chitosan nanoparticles: A review,” Processes, vol. 8, no. 9, Art. no. 1173, 2020, doi:10.3390/pr8091173.
33. E. A. El-Alfy, M. K. El-Bisi, G. M. Taha, and H. M. Ibrahim, “Preparation of biocompatible chitosan nanoparticles loaded with tetracycline, gentamycin and ciprofloxacin as a novel drug delivery system for improving the antibacterial properties of cellulose-based fabrics,” Int. J. Biol. Macromol., vol. 161, pp. 1247–1260, Oct. 2020, doi:10.1016/j.ijbiomac.2020.06.118.
34. M. Kong, X. G. Chen, K. Xing, and H. J. Park, “Antimicrobial properties of chitosan and mode of action: A state-of-the-art review,” Int. J. Food Microbiol., vol. 144, no. 1, pp. 51–63, Nov. 2010, doi:10.1016/j.ijfoodmicro.2010.09.012.
35. V. Coma, A. Martial-Gros, S. Garreau, A. Copinet, F. Salin, and A. Deschamps, “Edible antimicrobial films based on chitosan matrix,” J. Food Sci., vol. 67, no. 3, pp. 1162–1169, 2002, doi:10.1111/j.1365-2621.2002.tb09470.x.
36. I. M. Helander, E. L. Nurmiaho-Lassila, R. Ahvenainen, J. Rhoades, and S. Roller, “Chitosan disrupts the barrier properties of the outer membrane of gram-negative bacteria,” Int. J. Food Microbiol., vol. 71, no. 2–3, pp. 235–244, Dec. 2001, doi:10.1016/S0168-1605(01)00609-2.
37. L. Qi, Z. Xu, X. Jiang, C. Hu, and X. Zou, “Preparation and antibacterial activity of chitosan nanoparticles,” Carbohydr. Res., vol. 339, no. 16, pp. 2693–2700, Nov. 2004, doi:10.1016/j.carres.2004.09.007.
38. R. C. Goy, D. de Britto, and O. B. G. Assis, “A review of the antimicrobial activity of chitosan,” Polímeros, vol. 19, no. 3, pp. 241–247, 2009, doi:10.1590/S0104-14282009000300013.
39. M. Alves, I. C. F. R. Ferreira, J. Dias, V. Teixeira, A. Martins, and M. Pintado, “A review on antifungal activity of mushroom (basidiomycetes) extracts and isolated compounds,” Curr. Top. Med. Chem., vol. 13, no. 21, pp. 2648–2659, 2013, doi:10.2174/15680266113136660191.
40. L. Y. Ing, N. M. Zin, A. Sarwar, and H. Katas, “Antifungal activity of chitosan nanoparticles and correlation with their physical properties,” Int. J. Biomater., vol. 2012, Art. no. 632698, 2012, doi:10.1155/2012/632698.
41. A. M. Amani, R. Gholizadeh, S. R. Kasaee, Z. Zareshahrabadi, H. Kamyab, S. Chelliapan, et al., “Biological activities of chitosan–Schiff base metal (Ag and Zn) nanocomposites,” Results Chem., vol. 18, Art. no. 102717, 2025, doi:10.1016/j.rechem.2025.102717.
42. M. Z. Elsabee and E. S. Abdou, “Chitosan-based edible films and coatings: A review,” Mater. Sci. Eng. C, vol. 33, no. 4, pp. 1819–1841, May 2013, doi:10.1016/j.msec.2013.01.010.