Influence of Macerated and Cold Press Wheat Germ (Triticum vulgare) Oils Added at Different Rates to Trout Feed on The Feed Rate (FCR), Haemotology Parameters and NBT (Nitroblue tetrazolium) Values of Rainbow Trout (Oncorhynchus mykiss)

Abstract

In this study, 2% of macerated and cold press wheat germ oils were added to the feeds of rainbow trout (average weight of 39.8 ± 4 g). At the end of the study, growth rate, blood parameters and NBT (Nitro blue tetrazolium) level of rainbow trout were determined. Feed Conversion Ratios (FCR) of groups were determined as macerated: 1.12, cold press: 1.53, control: 1.11. Statistically significant differences were found in red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), white blood cell (WBC), platelet (PLT) values (p<0.05). There was no statistically significant difference between Nitroblue Tetrazolium (NBT) levels (p>0.05).

 

Keywords

• Blood parameters
• nitroblue tetrazolium
• trout
• wheat germ oil

Yemlere Farklı Oranlarda Eklenen Masere ve Soğuk Sıkım Buğday Tohumu (Triticum vulgare) Yağlarının, Gökkuşağı Alabalığı (Oncorhynchu smykiss)’nın Yem Değerlendirme Oranı (FCR), Hematolojik Parametreler ve NBT (Nitroblue tetrazolium) Değerlerinin Üzerine Etkisi

Abstract

Bu çalışmada, ortalama 39,8 ± 4 g ağırlığındaki gökkuşağı alabalıklarının yemlerine % 2 oranında masere ve % 2 oranında soğuk sıkım buğday tohumu yağı ilave edilmiştir. Çalışmanın sonunda, gökkuşağı alabalıklarının büyüme hızı, kan parametreleri ve NBT (Nitroblue tetrazolium) düzeyi belirlenmiştir. Grupların yem çevirme oranları (YÇO): Masere buğday yağı: 1,12, soğuk sıkım buğday yağı: 1,53, kontrol: 1,11 olarak tespit edilmiştir. Eritrosit (RBC), hemoglobin (HGB), hematokrit (HCT), lökosit (WBC), trombosit (PLT) değerlerinde anlamlı bir fark bulunmuştur (p<0,05). Nitroblue tetrazolium  (NBT)  düzeyleri arasında istatistiksel olarak anlamlı farkın olmadığı tespit edilmiştir (p> 0,05).

Keywords

• Kan parametreleri
• nitroblue tetrazolium
• alabalık
• buğday yağı

References

1. Abdou, H.M., Mohamed, N.A., El Mekkawy, D.A., EL-Hengary, SB., 2017. Vitamin E and/or wheat germ oil supplementation ameliorate oxidative stress induced by cadmium chloride in pregnant rats and their fetuses. Jordan Journal of Biological Sciences, 10(1):39–48.
2. Aboubakr, M., EL-Badawy, M., Soliman, A., El-Hewaity, M., 2014. Embryotoxic and teratogenic effects of norfloxacin in pregnant female albino rats. Advances in Pharmacological Sciences, Article ID 924706.
3. Altinterim, B.,Gulec, A.K., Aksu, O., 2012. Determination of safety dose of Eucalyptus camaldulensis hydrosol on mirror carp (Cyprinus carpio). Fresenius Environmental Bulletin, 21(5a):1219-1222.
4. Altinterim, B., Kutluyer, F., Aksu, O., 2018a. Effects of different plant oils having different oxygen radical absorbance capacity (ORAC) on hematological parameters of rainbow trout (Oncorhynchus mykiss) at high stocking density. Ataturk University Journal of Veterinary Sciences, 13(1):63-69.
5. Altinterim, B., Ozturk, E., Kutluyer, F., Aksu, O., 2018b. Effects of green tea (Camellia sinensis) oil on feed utilization and hametological parameters of rainbow trout (Oncorhynchus mykiss). Ataturk University Journal of Veterinary Sciences, 13(2):159-164.
6. Altinterim, B., Danabas, D., Aksu, O., 2018c. The effects of common yarrow (Achillea millefolium Linnaeus), cinnamon (Cinnamomum zeylanicum Blume) and rosemary (Rosemarinus officinalis Linnaeus) hydrosols on the some immunological and hematological parameters of common carp (Cyprinus carpio L., 1758) against to Yersinia ruckeri. Cellular and Molecular Biology, 64(14):19-24.
7. Blaxhall, P.C., 1972. The haematological assessment of the health of freshwater fish. A review of selected literature. Journal of Fish Biology, 4:593-604.
8. Blaxhall, P.C., Daisley, K.W., 1973. Routine haematological methods for use with fish blood. Journal of Fish Biology, 5:771-781.

9. Bouts, T., Gasthuys, F., 2003. The impotance of vitamin E in zoo mammals. Vlaams Diergeneeskundig Tijdschrift, 72:125-129.
10. Caruso, G., Genovese, L., Maricchiolo, G., Modic, A., 2005. Haematological, biochemical and immunological parameters as stress indicators in Dicentrarchus labrax and Sparus aurata farmed in off-shore cages. Aquaculture International, 13:67-73.
11. Chang, S.T., Chen, P.F., Chang, S.C., 2001. Antibacteriel activity of leaf essential oils and their constituents from Cinnamon osmophloeum. Journal of Ethanophermacology, 77:123-127.
12. Chorianopoulos, N.G., Giaouris, E.D., Skandamis, P.N., Haroutounian, S.A., Nychas, G.J.E., 2008. Disinfectant test against mono culture and mixed-culture bio films composed of technological, spoilage and pathogenic bacteria: bactericidal effect of essential oil and hydrosol of Satureja thymbra and comparison with standard acid base sanitizers. Journal of Applied Microbiology, 104:1586–1596.
13. Cicero, L.H., Barrella, W., Rotundo, M.M., 2014. Parâmetros hematológicos dos peixes: procedimentos para análise ambiental. Unisanta Bio Science, 3:50-63.
14. Ferreira, M.W., da Costa, D.V., Leal, C.A.G., Figueiredo, H.C.P., Rosa, P.V., 2015. Dietary oil sources on the innate immunity and resistance of Nile tilapia, Oreochromis niloticus, to Streptococcus agalactiae challenge. Journal of The World Aquaculture Society, 46(3):252-262.
15. Gāliņa, D., Valdovska, A., 2017. Effect of probiotics and herbals on health and shedding of resistant Escherichia Coli in piglets. Veterinary Medicine. Research for Rural Development, 1:251-258.
16. Gulec, A.K., Altinterim, B, Aksu, O., 2013. Determination of lethal concentration (LC50) values of Cinnamomum zeylanicum hydrosol on carp fish. Iranian Journal of Fisheries Sciences. 12(1):34-44.
17. Hoşsu, B., Korkut, A.Y., Fırat, A., 2001. Balık besleme ve yem teknolojisi I (Balık Besleme Fizyolojisi ve Biyokimyası). Ege Üniversitesi Su Ürünleri Fakültesi Yayınları, İzmir.
18. Kaushik, S.J., Cravedi, J.P., Lalles, J.P., Sumpter, J., Fauconneau, B., Laroche, M., 1995. Partialor total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic orantigenic effects, cholesterolemia and flesh quality in rainbow trout. Oncorhynchus mykiss. Aquaculture, 133:257–274.
19. Khalifa, F.K., Khalil, F.A., Barakat, H.A., Hassan, M.M., 2011. Protective role of wheat germ and grape seed oils in chlorpyrifos-induced oxidative stress, biochemical and histological alterations in liver of rats Australian. Journal of Basic and Applied Sciences, 5(10):54-66.
20. Kiron, V., Puangkaew, J., Ishizaka, K., Satoh, S., Watanabe, T., 2004. Antioxidant status and nonspecific immune responses in rainbow trout (Oncorhynchus mykiss) fed two levels of vitamin E along with three lipid sources. Aquaculture, 234:361-79.
21. Leenhardt, F., Fardet, A., Lyan, B., Gueux, E., Rock, E., Mazur, A., Chanliaud, E., Demigne, C., Remesy, C., 2008. Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. Journal of the American College of Nutrition, 27:222–228.
22. Niu, L.Y., Jiang, S.T., Pan, L.J., 2011. Preparation and evaluation of antioxidant activities of peptides obtained from defatted wheat germ by fermentation. Journal of Food Science and Technology, 50(1):1-9.
23. Omima, I.A., Hegazy, H.M.R., Fakhry, F.M., 2011. Amendment effect of antioxidants of barley and oat against teratogenicity induced by amritaz. Benha Veterinary Medical Journal, 1:35-43.
24. Ozkan, Y.F., Engin, K., Özlüer, H.A. 2015. The effects of balanced diets with soybean extract or meat and bone meal on muscle and liver tissue protein and glycogen levels of the Nile tilapia (Oreochromis niloticus L.) infected with Vibrio anguillarum. Journal of Applied Biological Sciences, 9:37.
25. Paranich, V.A., Cherevko, O.I., Frolova, N.A., Paranich, A.V., 2000. The effect of wheat germ oil on the antioxidant system of animals. Lik Sprava, 2:40–44.
26. Pelissero, C., Le Menn, F., Kaushik, S., 1991. Estrogenic effect of dietary soybean meal on vitellogenesis in cultured Siberian sturgeon Acipenser baeri. General and Comparative Endocrinology, 83:447–457.
27. Sitjà-Bobadilla, A., Peña-Llopis, S., Gómez-Requeni, P., Médale, F., Kaushik, S., Pérez-Sánchez, J., 2005. Effect of fish meal replacement by plant protein sources on non-specific defence mechanisms and oxidative stress in gilthead seabream (Sparus aurata). Aquaculture, 249:387–400.
28. Stehly, G.R., Gingerich, W.H., 1999. Evaluation of AQUI-S™ (efficacy and minimum toxic concentration) as a fish anaesthetic/sedative for public aquaculture in the United States. Aquaculture Research, 30:365–372
29. Tusche, K., Arning, S., Wuertz, S., Susenbeth, S., Schulz, C., 2012. Wheat gluten and potato protein concentrate promising protein sources for organic farming of rainbow trout (Oncorhynchus mykiss). Aquaculture, 344-349:120-125
30. Zacchi, P., Daghero, J., Jaeger, P., Eggers, R., 2006. Extraction/fractionation and deacidification of wheat germ oil using super critical carbon dioxide. Brazilian Journal of Chemical Engineering, 23:105-110.