Lemna minor L. (Su mercimeği)'de Everzol Lacivert ED’ye Dirençte Salisilik Asitin Rolü

Abstract

Tekstil endüstrisinin üretiminde kullanılan boyalar biyolojik uygulamalara dayanıklı toksik maddelerdir. Çoğunun oldukça karmaşık polimer yapıları vardır. Salisilik asit (SA), bitkiler tarafından üretilen, birçok biyotik ve abiyotik stres faktörüne tolerans sağlayan önemli bir hormondur. Su mercimekleri serbest yüzen bitkilerdir. Bu bitkiler, sucul ortamlara yüksek adaptasyonları, küçük biyokütleleri ve kirletici biriktirme kabiliyetleri gibi nedenlerle birçok araştırmacı tarafından laboratuarda toksikolojik çalışmalarda kullanılmıştır. Bu çalışmanın amacı 75 ppm, 150 ppm ve 300 ppm Everzol Lacivert ED (ELED) reaktif boyarmadde ile 0.5 mM SA ve ELED uygulamasının su mercimeğindeki (L. minor L.) bazı biyokimyasal değişiklikler değerlendirilmiştir. Toplam klorofil ve karotenoid içerikleri 7. günde 0.5 mM SA ve 300 ppm boya uygulanan gruplarda daha yüksek bulunmuştur. Peroksidaz (POD), askorbat peroksidaz (APX) ve Glutatyon S transferaz (GST) aktiviteleri ve toplam glutatyon (GSH) içeriği 0.5 mM SA ve ELED uygulanan gruplarda artmıştır. Süperoksit dismutaz (SOD) ve katalaz (CAT) aktivitesi her iki uygulama grubunda da 1., 4 ve 7. günlerde artış göstermiştir. GR aktivitesi hem boya uygulanan gruplarda hem de 0.5 mM SA ve boya uygulanan gruplarda 7. günde azalmıştır. MDA içeriği 7. günde 300 ppm boya uygulanan gruplarda ve 0.5 mM SA ve 300 ppm boya uygulanan gruplarda azalmıştır.

Keywords

• Lemna minor
• lipid peroksidasyonu
• pigmentasyon
• reaktif boyarmadde
• salisilik asit

Role of Salicylic Acid in Resistance to Everzol Navy ED in Lemna minor L. (Duckweed)

Abstract

The dyes used in the production of the textile industry are toxic substances that are resistant to biological treatment. Most of them have highly complex polymer structures. Salicylic acid (SA) is an important hormone produced by plants that provides tolerance to many biotic and abiotic stress factors. Duckweed (L. minor L.) are free floating plants. These plants have been used in laboratory toxicological studies by many researchers for reasons such as their high adaptability to aquatic environments, their small biomass and their high ability to accumulate contaminants. The aim of this study was to evaluate some biochemical changes in duckweed (L. minor L.) of 75 ppm, 150 ppm and 300 ppm Everzol Navy ED (ENED) reactive dyestuff and 0.5 mM SA and ENED application. Total chlorophyll and carotenoid contents were found higher at 0.5 mM SA and 300 ppm dye applied groups on day 7. Peroxidase (POD), ascorbate peroxidase (APX) and Glutathione S-transferase (GST) activities and total glutathione (GSH) content were increased at 0.5 mM SA with ENED application groups. (Superoxide dismutase) SOD and Catalase (CAT) activity increased on days 1, 4 and 7 in both treatment groups. GR activity decreased in dye applied groups and 0.5 mM SA with dye applied groups on day 7. MDA content decreased on day 7 with 300 ppm dye application and 0.5 mM SA and 300 ppm dye application.

Keywords

• Lemna minor
• lipid peroxidation
• pigmentation
• reactive dyestuff
• salicylic acid

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