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Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts

Year 2021, Volume 1, Issue 1, 48 - 51, 15.12.2021

Abstract

This study aimed to determine the anti-parasitic activity of the water extract obtained from Thymbra spicata (L.). The plant material was extracted with methanol in a Soxhlet apparatus. The extract was then fractionated with water and chloroform. The water phase was frozen and freeze-dried. Afterward, this extract was applied on A. castellani trophozoites and cysts at various concentrations, and the viability rates were determined by counting under the microscope. At the end of the experimental process, it was determined that there was a strong correlation between the increasing extract concentration and the anti-parasitic effect. T. spicata extract was not effective enough to neutralize all cysts at any of the concentrations examined. T. spicata extract, at concentrations of 16.0 and 32.0 mg/ml, removed all trophozoites in the medium from the 24th h of the experiment. Based on this result, it was determined that the plant species evaluated here could be used to treat A. castellani infections. It will be possible to achieve a good effect on the cyst forms of the parasite species by testing higher extract concentrations, which cannot be evaluated during the experimental process. In addition, with advanced analyzes, it will be possible to reveal the chemical substance responsible for the activity in the plant species in question and to perform further analyzes on this substance.

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Details

Primary Language English
Subjects Pharmacology and Pharmacy
Journal Section Research Articles
Authors

Baris GULEC This is me
ANADOLU UNIVERSITY
0000-0002-0271-0589
Türkiye


Duhan TORE This is me
DOGUS UNIVERSITY
0000-0001-7147-0817
Türkiye


Arzuhan SIHOGLU TEPE (Primary Author)
KILIS 7 ARALIK UNIVERSITY
0000-0001-8290-9880
Türkiye


Ibrahim Halil KILIC This is me
GAZIANTEP UNIVERSITY
0000-0002-0272-5131
Türkiye

Publication Date December 15, 2021
Application Date July 8, 2021
Acceptance Date July 17, 2021
Published in Issue Year 2021, Volume 1, Issue 1

Cite

Bibtex @research article { ijpbp975590, journal = {International Journal of Plant Based Pharmaceuticals}, issn = {}, eissn = {2791-7509}, address = {Kilis 7 Aralık Üniversitesi, Fen-Edebiyat Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, 79000, Merkez-KİLİS}, publisher = {Kilis 7 Aralik University}, year = {2021}, volume = {1}, pages = {48 - 51}, doi = {}, title = {Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts}, key = {cite}, author = {Gulec, Baris and Tore, Duhan and Sıhoglu Tepe, Arzuhan and Kılıc, Ibrahim Halil} }
APA Gulec, B. , Tore, D. , Sıhoglu Tepe, A. & Kılıc, I. H. (2021). Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts . International Journal of Plant Based Pharmaceuticals , 1 (1) , 48-51 . Retrieved from https://dergipark.org.tr/en/pub/ijpbp/issue/64282/975590
MLA Gulec, B. , Tore, D. , Sıhoglu Tepe, A. , Kılıc, I. H. "Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts" . International Journal of Plant Based Pharmaceuticals 1 (2021 ): 48-51 <https://dergipark.org.tr/en/pub/ijpbp/issue/64282/975590>
Chicago Gulec, B. , Tore, D. , Sıhoglu Tepe, A. , Kılıc, I. H. "Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts". International Journal of Plant Based Pharmaceuticals 1 (2021 ): 48-51
RIS TY - JOUR T1 - Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts AU - Baris Gulec , Duhan Tore , Arzuhan Sıhoglu Tepe , Ibrahim Halil Kılıc Y1 - 2021 PY - 2021 N1 - DO - T2 - International Journal of Plant Based Pharmaceuticals JF - Journal JO - JOR SP - 48 EP - 51 VL - 1 IS - 1 SN - -2791-7509 M3 - UR - Y2 - 2021 ER -
EndNote %0 International Journal of Plant Based Pharmaceuticals Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts %A Baris Gulec , Duhan Tore , Arzuhan Sıhoglu Tepe , Ibrahim Halil Kılıc %T Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts %D 2021 %J International Journal of Plant Based Pharmaceuticals %P -2791-7509 %V 1 %N 1 %R %U
ISNAD Gulec, Baris , Tore, Duhan , Sıhoglu Tepe, Arzuhan , Kılıc, Ibrahim Halil . "Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts". International Journal of Plant Based Pharmaceuticals 1 / 1 (December 2021): 48-51 .
AMA Gulec B. , Tore D. , Sıhoglu Tepe A. , Kılıc I. H. Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts. Int. J. Plant Bas. Pharm.. 2021; 1(1): 48-51.
Vancouver Gulec B. , Tore D. , Sıhoglu Tepe A. , Kılıc I. H. Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts. International Journal of Plant Based Pharmaceuticals. 2021; 1(1): 48-51.
IEEE B. Gulec , D. Tore , A. Sıhoglu Tepe and I. H. Kılıc , "Investigation of the anti-parasitic effect of the water extract of Thymbra spicata on Acanthamoeba castellani (L.) trophozoites and cysts", International Journal of Plant Based Pharmaceuticals, vol. 1, no. 1, pp. 48-51, Dec. 2021

Open Access Policy: This journal (IJPBP) adopted Budapest Open Access Initiative definition. The journal follows Committee on Publication Ethics (COPE) guidelines on the Code of Conduct and supports open access.

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