Enzymatic Saccharaification of Bagasse: Effects of Different Pre-Treatment Methods

Abstract

About 800,000 tons of bagasse is obtained per year as a by-product from sugar industries in Bangladesh. Bagasse is one of the most readily available lignocellulosic biomass that is a potential source for ethanol production through enzymatic saccharification and hydrolysis. Pretreatment enhances saccharification by removing lignin and hemicellulose. In this study pretreatment of bagasse was done using hot compressed water (HCW), (HCW+H3PO4) and (HCW+NH3). Effects of different pretreatment parameters such as temperature, residence time, concentration of NH3 and H3PO4 were studied in detail. (HCW+H3PO4) system was found to be more effective compared to other systems investigated in this study. A maximum yield of glucose (60%) and xylose (71%) was obtained in case of (HCW+H3PO4) system at temperature180°C and 160°C and residence time of 30 min and 15 min, respectively.

Keywords

• Saccharification, Bagasse, Biofuel, Pretreatment, Hot compressed water.

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